ChimeraX commands used for producing final movie (SVD and Gaussian filter) in “RyR1_Demo_Output.mp4”:

%%% E. Seitz, CU 2021

GUI: Load in all 50 NLSA volumes from ‘/post/2_svd’ directory
GUI: Graphics —> Full
CMD: camera ortho
CMD: volume #0-50 step 1
CMD: volume gaussian #0-50 sdev 1
GUI: Delete original volumes (“close”); keep new Gaussian filtered volumes
CMD: volume #0-200 level .0095
CMD: surface dust #0-200 size 50
CMD: volume #0-200 color 70,70,70
GUI: turn x -90
GUI-1: enable the first volume only
CMD-2: save 01.png width 1600 height 1600 supersample 3
{repeat GUI-1 and CMD-2 steps for each volume… there may be a built in way (?)}