Characterising a Hybrid Zone between a Cryptic Species Pair of Freshwater Snails

README for POPS_input.txt

This is the infile used in the POPS analyses.

Other POPS infiles are POPS_geo_dist_circle and POPS_predictionfile.txt.

This is a tab-delimited text file containing environmental data, spatial data and the nuclear marker genotypes at 9 microsatellite markers and 3 coding genes for 316 Radix snails 
from the core population data set. 

Note that the text file was created in Windows 8.1. The data has been formatted for use with the program POPS (http://membres-timc.imag.fr/Olivier.Francois/pops.html).

The first row contains self-explanatory headers indicating the contents of their respective columns. The subsequent rows contain climate data, spatial data and genotype data for each individual.

Column 1: Individual identifiers

All individuals are identified by the three letter population code given in Table S1, supporting information file [available at http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291365-294X]
followed by a number, e.g. ALS1.

Column 2: PC1_precipitation

The position of each individual along the first principal component axis, which is a precipitation gradient. All individuals from the same population have the same position.

Column 3: PC2_temperature

The position of each individual along the second principal component axis, which is a precipitation gradient. All individuals from the same population have the same position.

Columns  4 & 5: Longitude and Latitude, respectively.

GPS coordinates of each sample site. Note that the co-ordinates for each individual from one sample site have been ‘jittered’ by  +/-0.001 degrees.

Columns 6 – 23: Microsatellite marker genotypes.

Microsatellite alleles are given as fragment lengths in base pairs. -9 represents missing data. Each individual’s diploid genotype for each microsatellite marker is given in one row, 
so there are two columns per marker.

Columns 24 – 29: coding gene genotypes for act1a, HSPA2 and Psmd2.

Different alleles are given as haplotype number for act1a and ‘nested haplotype’ numbers for HSPA2 and Psmd2 (see figure S4, supporting information file available 
at http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291365-294X). -9 represents missing data. Again, there are two columns for each gene representing diploid genotypes. 
