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\f0\fs24 \cf0 Data file names and descriptions of vectors within each:\
\
Data S1 - infection prevalence and sex ratio through eight generations. Used to produce Figure_1, Figure_2 and Table_1\
	\'91Arsenophonus treatment\'92: Treatment level 1. Whether the symbiont was present in the population (infected) or not (uninfected) \
	\'92Superparasitism_permitted\'92: Treatment level 2. Whether females were allowed to oviposit in isolation (\'91NO\'92), or in groups of four (\'91YES\'92). \
	\'91Pupal_resource\'92 : Treatment level 3. Wasps were exposed to either four fly pupae for ovipositor (\'91High\'92), or one fly pupae (\'91low\'92) per patch within the population.\
	\'91Generation\'92: The generation (1-8) of the experiment these data were taken from.\
	\'91cohort\'92: The 6 replicate populations in each treatment were propagated in blocks, one week apart (A & B = blocks)\
	\'91Repicate_within_treatment\'92: numerical 1-6 identifiers for the six replicate populations within each treatment combination.\
	\'91Number_wasps_screened\'92: The number of female offspring produced in this generation that were PCR screened for the symbiont. Populations in the \'91uninfected\'92 treatment group were not screened.\
	\'91Number_infected\'92: The number of wasps screened as positive for symbiont infection.\
	\'91Number_wasps_sexed\'92: The total number of offspring in this generation that were sexed.\
	\'91Number_males\'92: Number of male offspring counted.\
\
Data S2 - Population viability through time. Used for Figure_3\
	\'91Arsenophonus_treatment\'92: as in Data 1\
	\'92Superparasitism_permitted\'92: as in Data 1\
	\'91Pupal_resource\'92: as in Data 1\
	\'91Generation\'92: As in Data 1\
	\'91Number_starting_population\'92: The number o replicate population in this treatment combination at the beginning of the experiment.\
	\'92Extant_populations\'92: The number of viable replica populations at this point (Generation) in the experiment.\
\
Data S3 - Infection prevalence at generation 4 for populations of N.vitripennis kept at variable opportunities to super parasitise. Used for Figure_4.\
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\cf0 	\'91
\f1 Proportion_Females_Superparasitising\'92: Proportion of the 40 female wasps that were kept int he same tube as 3 other females, thus being able to superparasitise.\
	\'91Infection_prevalence_@G0\'92: The proportion of females in the first generation of wasps that were infected with Arsenophonus nasoniae. Fixed at 50%, verified by PCR detection.\
	\'91Replicate_population_number\'92: Identifier for the three replicate populations in each treatment (integer: 1,2,3)\
	\'91Number_individuals_screened_@G4\'92: The number of female wasps screened for Arsenophonus infection by PCR in the fourth generation.\
	\'91Number_individuals_infected_@G4\'92: The number of individual females that screened positive for Arsenophonus in generation four.\
\
Data S4 - Multiple host species infection dynamics: Used in Figure 5\
	\'91Wasp_species\'92: Identity of wasp species, N.B. two separate lines of Nasonia vitripennis used (denoted 1 & 2).\
	\'91Within-species_population_replicate\'92: Identity of replicated populations with each species.\
	\'91Within-population_wasp_number\'92: Identity of female wasp individual, within each replicate population of each species.\
	\'91Arsenophonus_infection\'92: Infection status of the given wasp individual as given by PCR detection (1=infected, 0=uninfected)\
\
Data S5 - Vertical transmission efficiency of Arsenophonus nasoniae in five species of parasitoid wasps. Data collected after two generations of passage following Arsenophonus introduction. Used for Figure 6\
	\'91Species\'92: Host wasp species.\
	\'91Replicate_population\'92: The replicate population nested within species.\
	\'91Individual_female_ID\'92: The individuals female from within the \'91replicate population\'92. \
	\'91Mother_A+\'92: Infection status of mothers as verified by PCR. 1=infected, uninfected have been removed as they were not used to analyse vertical transmission efficiency.\
	\'91No_offspring_screened\'92: The number of female offspring of this female that were screened for Arsenophonus with PCR.\
	\'91No_offspring_positive\'92: The number of screened female offspring from this female that were positive for Arsenophonus 16s by PCR.\
	\'91VT Rate\'92: The efficiency of vertical transmission of Arsenophonus in this brood, calculated as the No_offspring_screened\'92/\'91No_offspring_positive\'92.\
	\
Data S6 - Cost of Arsenophonus nasoniae infection in multiple species: Data collected from 4 of 5 species after two generations of passage following Arsenophonus introduction. Used for Figure 7\
       \'91Species\'92: Host wasp species\
	\'91Replicate_population\'92: The replicate population nested within species\
	\'91Individual_female_ID\'92: The individual female from within the \'91replicate population\'92 for which daughter number was counted\
	\'91Average number of females uninfected\'92: The mean number of females produced by uninfected control females of this species\
	\'91delta females\'92: The difference between the observed sex ratio for the given female in the given replicate population of the given species and the mean number of daughters produced by uninfected female so this species (from control populations that never carried the symbiont).\
\
Data S7 - Male killing efficiency of Arsenophonus strain CAN1 in host wasp Nasonia vitripennis\
	\'91cohort\'92: Experimental block\
	Mother Arsenophonus status: Symbiont infection status of mother as verified by PCR screening. \'91A+\'92 = infected.\
	Female.offspring: Number of females produced by given mother\
	Male.offspring: Number of males produced by given mother\
\
Data S8 - Male killing efficiency of Arsenophonus nasonaie strain UK1 in multiple host wasp species.\
	Wasp_species: Host wasp species ID\
	Arsenophonus_status_mother: Symbiont infection status of mother as verified by PCR screening.\
	number_Female_offspring: Number of females produced by given mother\
	number_male_offspring: Number of males produced by given mother\
\
Data S9 - Superparasitism intensity of Nasonia vitripennis at varying wasp and host densities.\
	\'91Wasp density\'92: Number of female wasps in a vial\
	\'91fly_pupa_density\'92: Number of dipteran pupa in a vial\
	\'91order_of_pupa_within_treatment\'92: Individual identifier for pupae within each vial (\'91one\'92 - \'91four\'92).\
	\'91number_Female_offspring\'92: Number of females produced by given mother\
	\'91number_male_offspring\'92: Number of males produced by given mother\
	\'91significantly female biased?\'92: Was the given clutch statistically significantly biased at 95% threshold? 0=no, 1=yes.\
	}