
Information for 'MS-AFLP_data.xlsx' file


HerB = Herslev benthic		
HerNR = Herslev non-reproductive		
RoP = Rm planktonic		
RoNR = Rm non-reproductive		



SHEET: msap_transformed.MSL

Data file transformed with msap [1], methylation scoring from [1,2]
165 loci (m1-m165)

Methylation-sensitive loci (MSL):

1: unmethylated
2: methylated (including both the methylated and hemimethylated state)
NA: uninformative



SHEET: msap_calc_Mix2_transformed data


Data file transformed with R script MSAP_calc [3] using the mixed scoring transformation (Mixed Scoring 2) 
395 epiloci

- u: unmethylated
- m: methylated
- h: hemimethylated

 

1. Prez-Figueroa  A. MSAP: a tool for the statistical analysis of methylation-sensitive amplified polymorphism data. Mol Ecol Resour. 2013;13: 522527.
2. Herrera CM, Bazaga P. Epigenetic differentiation and relationship to adaptive genetic divergence in discrete populations of the violet Viola cazorlensis. New Phytol. 2010;187: 867876.
3. Schulz B, Eckstein RL, Durka W. Scoring and analysis of methylation-sensitive amplification polymorphisms for epigenetic population studies. Mol Ecol Resour. 2013;13: 642653.


 