This file explains all of the variables in each of the datasets that accompany: Johnson et al. 2017 ms. Evidence that fertility trades off with early offspring fitness as males age

Cohort-based longitudinal analyses:

dataset: MaleSurvival.csv
Age	=	age of males when sampling (months)
Tank	=	tank fish housed in, ML6B1, ML6B2 or ML6B3
Survived	=	number of males alive at sampling
x.Survived	=	number of males dad at sampling (from total of 15)
P_Survived	=	proportion of males surviving

dataset: MatingSuccess.csv
Age	=	age of males when sampling (months)
Tank	=	tank fish housed in, ML6B1, ML6B2 or ML6B3
Mated	=	number of surviving males that mated (i.e., fertilised eggs produced) during mating interval
x.Mated	=	number of surviving males that did not mate during mating interval
P_Mated	=	proportion of males that mated

dataset: SpermProduction.csv
Age	=	age of males when sampling (months)
Tank	=	tank fish housed in, ML6B1, ML6B2 or ML6B3
VOL	=	male ejaculate volume, categorised into 4 levels (0=no semen, 1=0-1 uL, 2=1-3 uL, 3=3+ uL)
VOL_binary	=	male ejaculate collected at sampling (0 = no, 1 = yes)

dataset: SpermConcentration.csv
Age	=	age of males when sampling (months)
Tank	=	tank fish housed in, ML6B1, ML6B2 or ML6B3
FishID_unique	=	male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID
Sperm_mL	=	concentration of sperm per mL
Size = total male length (cm)

dataset: CASA.csv
Age	=	age of males when sampling (months)
Tank	=	tank fish housed in, ML6B1, ML6B2 or ML6B3
FishID_unique	=	male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID
VAP	=	sperm swimming speed (actual path velocity)
VSL	=	sperm swimming speed (straight line velocity)
VCL	=	sperm swimming speed (curvilinear velocity)
MOTILE_PCT	=	percentage of motile sperm in sample
Size = total male length (cm)

dataset: IVF.csv
Age	=	age of males when sampling (months)
Tank	=	tank fish housed in, ML6B1, ML6B2 or ML6B3
FishID_unique	=	male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID
Developed	=	number of developed embryos at 24 hours post fertilisation
x.Developed	=	number of undeveloped embryos at 24 hours post fertilisation
P_Developed	=	sperm swimming speed (curvilinear velocity)
Hatch	=	number of developed embryos (@ 24 hpf) that hatched by 5 days
x.Hatch	=	number of developed embryos that did not hatch by 5 days
P_Hatch	=	proportion of developed embryos (@ 24 hpf) that hatched by 5 days
Size = total male length (cm)

dataset: ProgenySurvival.csv
Age	=	age of males when sampling (months)
Tank	=	tank fish housed in, ML6B1, ML6B2 or ML6B3
FishID_unique	=	male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID
Survived	=	number of developed embryos (@ 24hpf) that survived to 15 days
x.Survived	=	number of developed embryos (@ 24 hpf) that did not survive to 15 days
P_Survived	=	sperm swimming speed (curvilinear velocity)

Cross-sectional (young vs. old males) analyses:

dataset: CASA_CS.csv
Age	=	age of males when sampling (months)
FishID_unique	=	male ID
VAP	=	sperm swimming speed (actual path velocity)
VSL	=	sperm swimming speed (straight line velocity)
VCL	=	sperm swimming speed (curvilinear velocity)
MOTILE_PCT	=	percentage of motile sperm in sample

dataset: IVF_CS.csv
Age	=	age of males when sampling (months)
FishID_unique	=	male ID
Developed	=	number of developed embryos at 24 hours post fertilisation
x.Developed	=	number of undeveloped embryos at 24 hours post fertilisation
P_Developed	=	sperm swimming speed (curvilinear velocity)
Hatch	=	number of developed embryos (@ 24 hpf) that hatched by 5 days
x.Hatch	=	number of developed embryos that did not hatch by 5 days
P_Hatch	=	proportion of developed embryos (@ 24 hpf) that hatched by 5 days

dataset: ProgenySurvival_CS.csv
Age	=	age of males when sampling (months)
FishID_unique	=	male ID
Survived	=	number of developed embryos (@ 24hpf) that survived to 15 days
x.Survived	=	number of developed embryos (@ 24 hpf) that did not survive to 15 days
P_Survived	=	sperm swimming speed (curvilinear velocity)


