FastQCFastQC Report
Wed 6 Mar 2019
merged_060319_f75.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemerged_060319_f75.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16004690
Sequences flagged as poor quality0
Sequence length157-418
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACCGGCGCACGGGTGAGTAACGCGTATCCAACCTTCCCATTACTGTG10412536.5059242009685905No Hit
CGAACGGGTGAGTAACGCGTAGGTAACCTGCCTGGTAGCGGGGGATAACT9965656.226706046790035No Hit
GGCGGACGGGTGAGTAACACGTGGATAACCTACCTATAAGACTGGGATAA8154105.094819081156836No Hit
GTGGCGAACGGGTGAGTAACGCGTAATCAACCTGCCCTTCAGAGGGGGAC7290384.555152270990566No Hit
GACGGGTGAGTAATGCCTAGGAATCTGCCTGGTAGTGGGGGATAACGTCC5171153.231021656776857No Hit
GACCGGCGCACGGGTGCGTAACGCGTATCAAACCTGCCTCATACTCGGGG4399092.748625559132979No Hit
TCGACCGGCGCACGGGTGAGTAACGCGTATCCAACCTTCCCATGACTAAG4277242.6724916258921603No Hit
GACCGGCGCACGGGTGCGTAACGCGTATGCAACTTGCCTCACAGAGGGGG3244412.0271620381275737No Hit
GTGGCGAACGGGTGAGTAATATATCGGAACGTACCGAGTAATGGGGGATA2821551.762951984699485No Hit
TCGACCGGCGCACGGGTGAGTAACGCGTATCCAACCTACCCATTACTGTG2769931.7306989388735428No Hit
GACCGGCGCACGGGTGAGTAACGCGTATCCAACCTGCCTCTGACTAAGGG2609441.6304220825270592No Hit
GTGGCGAACGGGTGAGTAACATATCGGAACGTACCGAGTAATGGGGGATA1865301.1654708713508353No Hit
GGCGAACGGGTGAGTAACACGTGGGTAACCTGCCCATCAGAGGGGGATAA1831911.1446082367106143No Hit
GGTGCGTAACGCGTATGCAATCTACCTTTTACAGAGGGATAGCCCAGAGA1785471.115591742170576No Hit
TCGACCGGCGCACGGGTGAGTATCGCGTATCCAACCTGCCCTTTACTTGG1753591.0956725809747019No Hit
CGGGTGAGTAACACGTGGGTGATCTGCCCTGCACTCTGGGATAAGCCTGG1704831.0652065113413631No Hit
CGAACGGGTGAGTAACACGTGAGTAACCTGCCCTTGACTTTGGGATAACT1624041.0147275579845658No Hit
TGCGAACGGGTGAGTAACGCGTAGGTAACCTGCCTCTTAGCGGGGGATAA1608061.004742984712606No Hit
TGCGAACGGGTGAGTAACGCGTAGGTAACCTGCCTGGTAGCGGGGGATAA1470910.9190493536582088No Hit
CGAACGGGTGAGTAACACGTGAGCAATCTGCCCTTGACTCTGGGATAACT1462560.9138321329560273No Hit
GACGGGTGAGTAATGCCTAGGAATCTGCCTGGTAGTGGGGGATAACGTTC1386560.8663460523134157No Hit
CGAACGGGTGAGTAATACATCGGAACATGTCCTGTAGTGGGGGATAGCCC1215570.759508619036045No Hit
GTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATA1169440.730685817719681No Hit
GTGGCGAACGGGTGAGTAACATATCGGAACGTACCGAGTAGTGGGGGATA1011190.6318085511184535No Hit
TGCGAACGGGTGAGTAACGCGTAGGTAACCTGCCTTGTAGCGGGGGATAA871200.5443404402084638No Hit
AACGGGTGAGTAATACGTGAGTAACCTACCTTTAACTCTGGGATAAGCCT869930.5435469228082518No Hit
CGAACGGGTGAGTAACACGTGGGTGACCTGCCCTGCACTTCGGGATAAGC848530.5301758422062532No Hit
AACGGGTGAGTAACACGTGGGTGATCTGCCTTGCACTTCGGGATAAGCCT825650.5158800326654249No Hit
GCGGCGAACGGGTGAGTAACACGTAAAGAACCTGCCTCATAGACTGGGAC757440.473261275288681No Hit
ACACGTATGCAACCTACCTTACATTGGGGGATAGCCTTTCGAAAGGGAGA756840.4728863851783446No Hit
TAACACGTGCTTAATCTACCTTTTAGATTGGAATACCCATTGGAAACAAT748700.4678003760147807No Hit
TCGACCGGCGCACGGGTGAGTAACGCGTATCCAACCTTCCCATAACTAAG744880.4654135756456389No Hit
CGCGTAAAGAACTTGCCTCACAGTTAGGGACAACATTTGGAAACGAATGC743780.4647262771100221No Hit
TAGTGGCAAACGGGTGAGTAACGCGTAAACAACCTGCCCTACGGATGGGG724220.4525048595130553No Hit
TAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAGACT700690.4378029190193624No Hit
AACGGGTGAGTAACACGTGAGTAACCTGCCCCCTTCTTTGGGATAACGCC690330.43132981644755386No Hit
GTGGCGGACGGGTGAGTAATGCTTGGGAATCTAGCTTATGGAGGGGGATA653560.40835530085243765No Hit
GACCGGCGCACGGGTGAGTAACGCGTATCCAACCTACCCAATACTCAGGC624950.39047929075789656No Hit
CGCACGGGTGAGTAATGTATAGCTAATCTGCCCTACACTAGAGGACAACA558720.3490976707452628No Hit
GTGGCGGACGGGTGAGTAATGCTTGGGAATCTGGCTTATGGAGGGGGATA522000.32615439599267465No Hit
GACCGGCGCACGGGTGAGTAACACGTATCCAACCTGCCCTTTACTCGGGG514030.32117460569370604No Hit
TCGACCGGCGCACGGGTGAGTAACGCGTATCCAACCTTCCCGTTACTGCG510290.3188377906726091No Hit
CGGGTGCGTAACGCGTATACAATCTACCTTTTACAGAGGGATAGCCCAGA496230.3100528657537259No Hit
GTGGCGAACGGGTGAGTAACGCGTAATCAACCTGCCCATCAGAGGGGGAC493560.30838460476272894No Hit
AACGGGTGAGTAACACGTGAGTAACCTGCCCTCTTCTTTGGGATAACGCC419930.2623793400559461No Hit
GACCGGCGCACGGGTGAGTAACGCGTATCCAACCTGCCTCTGACTGAGGG418470.2614671074541275No Hit
GGCAAACGGGTGAGTAACACGTAGACAACCTGCCGACAGGATGGGGACAA400880.25047657905276516No Hit
GTGGCGAACGGGTGAGTAACATATCGGAACGTACCGAGCAGTGGGGGATA391810.2448094902181798No Hit
ACGCGTGGGAATCTGCCCTTGGGTTCGGAATAACAGTTAGAAATGACTGC388480.24272885010581274No Hit
GCGGCGAACGGGTGAGTAACACGTAAAGAACCTGCCTCATAGACGGGGAC378890.2367368565089358No Hit
ACGCGTGGGAACCTGCCCTTAGGTTCGGAATAACTCCCCGAAAGGGGTGC374730.23413761841060338No Hit
AAGTGGATTAGTGGCGAACGGGTGAGTAACACGTGAGTAACCTGCCTTAC360280.2251090149200016No Hit
CGAACGGGTGAGTAATACATAAGCAACCTACCCACGAAGACTGGGATAAT355840.22233482810351213No Hit
AACACGTATCCAACCTACCCTTAAGTTGGGGATAACTAGTCGAAAGATTA345950.21615538945146703No Hit
CTGTGAGTTGCGAACGGGTGAGTAACGCGTAGGTAACCTGCCTGGTAGCG325460.2033528921834787No Hit
CGAACGGGTGAGTAACACGTGAGTAACCTGCCCCCTTCTTTGGGATAACG321420.20082863210721358No Hit
ACGGGTGAGTAACGCGTAAGGAACTTGCCCTGCAGTCAGGGATAACAGAC312420.19520528045216748No Hit
GCGGCGGACGGGTGAGTAATGTATGGGGATCTGCCTGATGGCGGGGGATA307640.1922186559064874No Hit
CGAACGGGTGAGTAACACGTGAGTAACCTGCCCTCTTCTTTGGGATAACG306090.19125018978811836No Hit
GTGGCAAACGGGTGAGTAACACGTAGACAACCTGCCGACAGGATGGGGAC297680.18599548007490305No Hit
CGTAACGCGTATACAATCTGCCTTTCACATAGGGATAGCCCGAAGAAATT296480.18524569985423023No Hit
CGGGTGAGGAACACGTGAGAAATCTGCCTTCAACTCTGGGATAACTCCGG293870.18361492787426686No Hit
TTTATTTCGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCAT293250.18322754142691922No Hit
AACGGGTGAGTAATATATCGGAACGTGCCCAGTCGTGGGGGATAACGTAG288950.18054082896950832No Hit
GACCGGCGCACGGGTGAGTAACGCGTACCGAACCTGCCCATCACACAGGG286420.17896004233758978No Hit
TCGACCGGCGCACGGGTGAGTAACGCGTATCCAACCTACCCATTACTGTT269280.16825068151897973No Hit
CGTTATCTAGTGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTTATG265960.1661762895751183No Hit
TTAGGAAAGCGAGCGGCGGATGGGTGAGTAACACGTGGGGAACCTGCCCC265110.16564519525214172No Hit
CGAACGGGTGAGTAACGCGTAGGTAACCTGCCTGGTAGTGGGGGATAACT264400.1652015752882436No Hit
AACGGGTGAGTAACACGTGGGCAACCTGCCCCAGACTCTGGGATAACCTC260960.1630522053223149No Hit
AACACGTATCCAATCTACCCTTAAGTTGGGGATAACTAGTCGAAAGATTA256560.1603030111798479No Hit
AACGGCTGAGTAACACGTGGGCAACCTGCCCCTCTCATTGGGATAGCCAC256180.16006558077663485No Hit
AACGGGTGAGTAATACGTGAGTGACCTACCTTTGACTCTGGGATAAGCCT251880.15737886831922393No Hit
TCGACCGGCGCACGGGTGAGTAACGCGTATCCAACCTTCCCACAACTAAG244210.1525865230754235No Hit
GGCGGACGGGTGAGTAACACGTGGGTAACCTACCTATAAGACTGGGATAA241450.15086202856787603No Hit
CGCGTAAAGAACTTGCCTCACAGATAGGGACAACATTTGGAAACGAATGC236040.14748176940634275No Hit
GACGGGTGAGTAACGCGTAAAGAACTTGCCTCACAGCTAGGGACAACATT231070.1443764296590562No Hit
GGCGGACGGGTGAGTAACACGTGGATAACCTACCTATAAGGCTGGGATAA226700.14164598002210602No Hit
CGGGTGAGGAACACGTGAAGAATCTGCCTTCAACTCTGGGATAACTCCGG223350.13955284357272774No Hit
AACGGGTGAGTAATACATCGGAACGTGCCCAGTAGTGGGGGATAGCTCGG220760.13793456792977557No Hit
TAAGATGGATTAGCGGCGGACGGGTGAGTAACACGTGAGTAACCTGCCTT215810.13484172451950022No Hit
CGAAATGAAAGTGGCGAACGGGTGAGTAACACGTGAGCAACCTACCTTAC211690.13226747909519024No Hit
AACGGGTGAGTAATACGTGAGTAACCTACCTTTAACTCTGGGATAAGCCC209290.1307679186538446No Hit
CGACCGGCGCACGGGTGAGTAACGCGTATCCAACCTTCCCATAACTAAGG193270.1207583527078625No Hit
CGGGTGCGGAACACGTGTGCAACCTGCCTTTATCTGGGGGATAGCCTTTC193050.12062089300073917No Hit
GACCGGCGCACGGGTGAGTAACGCGTATCCAACCTGCCCATTACAGGGGA189610.11847152303481041No Hit
ATGAAACTAGATACAAGCGAGCGGCGGACGGGTGAGTAACACGTGGGTAA188320.11766550929758715No Hit
ACGCGTGGGAATCTGCCCCTTGGTTCGGAATAACAGTTGGAAACGACTGC186540.11655333530358913No Hit
TAACACGTGCTTAATCTACCTCTTAGATTGGAATACCTAATGGAAACATT178320.11141734079198036No Hit
GGTGCGTAACGCGTATGCAATCTACCTTTCACAAAGGGATAGCCCAGAGA176900.11053010086418419No Hit
CGAACGGGTGAGTAATACATCGGAACGTACCTTATCGTGGGGGATAACGC168750.10543784353211465No Hit
GATTGATTTTGGTCGCCAACGAGTGGCGGACGGGTGAGTAACACGTAGGT167870.10488800470362125No Hit
AACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAGCTGGAAACGGCTG167130.10442564023420635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)