FastQCFastQC Report
Wed 6 Mar 2019
merged_060319.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemerged_060319.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16004690
Sequences flagged as poor quality0
Sequence length231-492
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGGCA184477611.52647130309928No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAG9502455.937290881610328No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGCGAT6863784.28860540254138No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGACGGCAGCACAG5401613.3750169481570715No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGAAGGG4818523.010692490763645No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACGGACGA4276542.6720542540967682No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACAGACGA3851592.406538333451007No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGT3113441.9453297752096417No Hit
GATGAACGCTAGCGATAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG3094991.933801904316797No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCC2868431.7922433986537696No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA2766741.7287057731202542No Hit
ATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGCAGCACGG2178961.361450924697698No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGTATAGTTC1890761.1813787083661103No Hit
GATGAACGCTAGCTATAGGCTTAACACATGCAAGTCGAGGGGAAACGGCA1842341.1511250764619623No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCT1800851.1252014253321996No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGCTT1764921.1027517558915543No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAGAGCGACC1738901.0864940214399654No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCTG1417820.8858778270619424No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCCT1283670.8020586465592273No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGAGAGA1215210.7592836849698432No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA1206940.7541164496157063No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA1166090.7285926812703026No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAAGTTTTTC1157050.7229443369412342No Hit
AACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGAGACCTTCG815760.5097005940133797No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG813160.508076070201922No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTAAGGCTC811100.5067889474897671No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGCGTAG732670.45778456190029304No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCCT706970.4417267688408835No Hit
GATGAACGCTAGCGGCAGGCCTAATACATGCAAGTCGAACGGTGTCTTCG698430.4363908329370953No Hit
GATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAGGTTAGCA685780.42848689977750276No Hit
GATGAACGCTGACAGAATGCTTAACACATGCAAGTCTACTTGAACTTCGG654930.4092112999377058No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGGAAGTAGTA645450.4032880361943905No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACATAA634700.3965712550508632No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGT594730.37159732553395286No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAATCGAACGAAGCCTTTT588300.3675797531848477No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCTGAAGCCC588130.36747353432025237No Hit
ATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGTAACAGGT534490.33395835845617755No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAG534090.33370843171595327No Hit
GATGAACGCTGGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGGCA529630.33092174856245266No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA509570.31838792254020537No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACCGACAT507610.31716328151310647No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCGAATG485590.30340481446376033No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAGCAGGA470770.29414502873845105No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGCTT448060.279955438062218No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCACAG443120.27686884282044827No Hit
AGTGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGGACAAGTAA421500.26336030251132636No Hit
GATGAACGCTGGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACG397320.24825223106476912No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGCGG382330.23888622647486457No Hit
GATAAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAACTT370960.23178205888398964No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGTAGAAGCA368470.23022626492609352No Hit
GATGAACGCTGACAGAATGCTTAACACATGCAAGTCAACTTGAACTTCGG347500.21712385556983607No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGATTTTTG332060.20747668339717917No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGGA330390.20643323925674284No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGATGAAGCAC315030.19683605243213084No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTGGAGATC301860.18860721451024667No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGGGCGGTAACAGGG287380.17955986651412803No Hit
GATGAACGCTGACAGAATGCTTAACACATGCAAGTCTTTGGCGAAGCTGT267140.16691357345877988No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGTGGGGCAGCGGAT264960.1655514727245576No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTGAACCAG262450.1639831824296503No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGCGGAACT261680.1635020734547186No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAA260530.1627835340765738No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGATTAAAGCA251740.15729139396014544No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCTGAAGACC247290.1545109589751504No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGATGAAACAC244120.15253028955887307No Hit
ATTGAACGCTGGCGGAATGCTTTACACATGCAAGTCGAACGGCAGCACGG238430.1489750816791828No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAAGTGAA227190.14195214027888076No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACAGACAA220340.13767214485254012No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATG215940.13492295071007313No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGATGCGTA215830.13485422085651144No Hit
GACGAACGCTGGCGGCGCGCTTAACACATGCAAGTCGAGCGATGAAATCT215510.134654279464332No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGA208330.13016809447730635No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAACGAT203270.1270065212134693No Hit
ATTGAACGCTGGCGGAATGCTTTACACATGCAAGTCGAACGGTAGAGGGG203070.12688155784335717No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGCTTGCCT196120.12253908073196045No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCTGAAGGCC191660.11975239757845982No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGGAG187760.11731561186127316No Hit
ATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGTAGAGTAG181240.11324180599561753No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAATACCA181170.11319806881607827No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGGGA176870.11051135635866736No Hit
GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGGACGGGAAGTGGT175940.10993027668764593No Hit
GATGAACGCTGGCGGCGTGCCTAAGACATGCAAGTCGAGTGGGTTTAGAC173880.10864315397549093No Hit
GATTAACGCTGGCGGCGTGCATAACACATGCAAGTCGAACGATGAAACTT173550.10843696441480591No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGACGGCAGCGGGG172580.10783089206976207No Hit
GATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGGAAGTAGCA171990.10746225012793124No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTGCTA162410.10147650469955995No Hit
GATGAACGCTAGCGGGAGGCCTAACACATGCAAGCCGAGCGGTATTGTTT160440.1002456155039554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)