
*************** ReadMe file accompanying data files for:


Social immunity modulates competition between coinfecting pathogens
by Barbara Milutinovic, Miriam Stock, Anna V. Grasse, Elisabeth Naderlinger, Christian Hilbe, Sylvia Cremer,
published in Ecology Letters

Email address corresponding authors: barbara.milutinovic@ist.ac.at, sylvia.cremer@ist.ac.at

All experimental procedures are described in the main manuscript text or supplemental material and are accompanied by the figure number they refer to.
Data collectors: Barbara Milutinovic, Miriam Stock, Anna V. Grasse, Elisabeth Naderlinger


*************** Data Files (.csv format):

1. Milutinovic_Fig1a.csv: Pathogen-induced host mortality (virulence)
	Columns:
	"host_context": context in which exposed ants were kept after exposure; Individual - alone, Group - with two unexposed nestmates
	"coinfection_diversity": diversity of strains in the coinfections: Same-species - two strains of same species, Different-species - two strains of different species
	"strain_combination": combination of the two strains (out of six strains) used for confection: Metarhizium brunneum B1, B2, B3; Metarhizium robertsii R1, R2, R3   
	"mortality": ant mortality after exposure to fungal strains; 1 - dead, 0 - alive
	This file is also used to create FigS1

2. Milutinovic_Fig1b_Fig2a.csv: Total spore production (Fig1b) and co-sporulation (Fig2a) on cadavers
	Columns:
	"host_context": context in which exposed ants were kept after exposure; Individual - alone, Group - with two unexposed nestmates
	"coinfection_diversity": diversity of strains in the coinfections: Same-species - two strains of same species, Different-species - two strains of different species
	"strain_combination": combination of the two strains (out of six strains) used for confection: Metarhizium brunneum B1, B2, B3; Metarhizium robertsii R1, R2, R3   
	"mixed_outgrowth": cadavers showing co-sporulation of both pathogens; yes - from both coinfecting strains, no - only one strain
	"outgrowing_strains": identity of the outgrowing strain (B1, B2, B3, R1, R2, R3) for single outgrowth, "Both" for outgrowth of both coninfecting strains
	"DNA_amount (ng/uL)": total DNA amount (ng/l) of the spores collected from per sporulating cadaver (measured by Qubit fluorometer)
	"spore_number": total spore number per cadaver, calculated from the DNA concentration using the standard curve
	This file is also used to create FigS2 and Fig S3

3. Milutinovic_Fig2b.csv: Sporulation success of individual fungal strains
	Columns:
	"host_context": context in which exposed ants were kept after exposure; Individual - alone, Group - with two unexposed nestmates
	"coinfection_diversity": diversity of strains in the coinfections: Same-species - two strains of same species, Different-species - two strains of different species
	"experimental_dish": replicate dish in which ants were kept during the experiment.
	"strain_combination": combination of the two strains (out of six strains) used for confection: Metarhizium brunneum B1, B2, B3; Metarhizium robertsii R1, R2, R3     	
	"focus_strain": strain (B1, B2, B3, R1, R2, R3), for which the outgrowth is considered in the "outgrowth_focus_strain" column	
	"non_focus_strain": partner strain (B1, B2, B3, R1, R2, R3), for which the outgrowth is not considered in the "outgrowth_focus_strain" column*
	"outgrowth_focus_strain": outgrowth of fungal strains from ant cadaver; 1 - successful, 0 - not successful

	*) Note that each pathogen combination is included twice with each strain once being the focus strain, once being the non-focus strain.


4. Milutinovic_Fig3ab.csv: Spore number retrieved from the ant after grooming
	Columns:
	"host_context": context in which exposed ants were kept after exposure; Individual - alone for 2h after exposure, Group - with two unexposed nestmates for 2h after exposure; 
			Baseline: immediately after exposure (applied dose) 
	"ant_ID": ant number in the experiment 
	"fungal species": B - Metarhizium brunneum, R - Metarhizium robertsii
	"qPCR_value": measure of species-specific real-time PCR value of the DNA concentration (ng/l) obtained from the spores on the ant
	"spore_number": species-specific spore number per ant, calculated from the DNA concentration using the standard curve


5. Milutinovic_Fig4.csv: Fungal germination duration
	Columns:
	"strain": fungal strain identity: Metarhizium brunneum B1, B2, B3; Metarhizium robertsii R1, R2, R3
	"replicate": replicate measure for each strain
	"germination_onset": time to germination onset [h] of the fungal strain in fungal growth medium, as identified by first breakpoint analysis (change from stationary to exponential growth phase)




	