Datasetw6a_vs_wt_vs_wt.w6a_vs_wt.cls
#ago1w6a_versus_ago1gfp.w6a_vs_wt.cls
#ago1w6a_versus_ago1gfp_repos
Phenotypew6a_vs_wt.cls#ago1w6a_versus_ago1gfp_repos
Upregulated in classago1w6a
GeneSetHALLMARK_INFLAMMATORY_RESPONSE
Enrichment Score (ES)0.47660065
Normalized Enrichment Score (NES)1.8628486
Nominal p-value0.0
FDR q-value2.5735295E-4
FWER p-Value0.001
Table: GSEA Results Summary



Fig 1: Enrichment plot: HALLMARK_INFLAMMATORY_RESPONSE   
Profile of the Running ES Score & Positions of GeneSet Members on the Rank Ordered List

PROBEDESCRIPTION
(from dataset)
GENE SYMBOLGENE_TITLERANK IN GENE LISTRANK METRIC SCORERUNNING ESCORE ENRICHMENT
1BST2NABST2
Entrez,  Source
bone marrow stromal cell antigen 224.9810.0178Yes
2TLR2NATLR2
Entrez,  Source
toll-like receptor 2714.6100.0305Yes
3TNFSF10NATNFSF10
Entrez,  Source
tumor necrosis factor (ligand) superfamily, member 10864.5590.0461Yes
4ICOSLGNAICOSLG
Entrez,  Source
inducible T-cell co-stimulator ligand884.5560.0624Yes
5EREGNAEREG
Entrez,  Source
epiregulin974.5280.0782Yes
6PCDH7NAPCDH7
Entrez,  Source
BH-protocadherin (brain-heart)1064.4910.0938Yes
7CSF1NACSF1
Entrez,  Source
colony stimulating factor 1 (macrophage)1094.4870.1098Yes
8NDPNANDP
Entrez,  Source
Norrie disease (pseudoglioma)1154.4470.1255Yes
9IL1R1NAIL1R1
Entrez,  Source
interleukin 1 receptor, type I1244.4100.1409Yes
10LPAR1NA1354.3400.1559Yes
11SELLNASELL
Entrez,  Source
selectin L (lymphocyte adhesion molecule 1)1464.3090.1708Yes
12IL6NAIL6
Entrez,  Source
interleukin 6 (interferon, beta 2)1484.3010.1862Yes
13CMKLR1NACMKLR1
Entrez,  Source
chemokine-like receptor 12553.8820.1942Yes
14P2RY2NAP2RY2
Entrez,  Source
purinergic receptor P2Y, G-protein coupled, 22613.8710.2078Yes
15LAMP3NALAMP3
Entrez,  Source
lysosomal-associated membrane protein 32983.7380.2192Yes
16SLC4A4NASLC4A4
Entrez,  Source
solute carrier family 4, sodium bicarbonate cotransporter, member 43003.7340.2325Yes
17TNFRSF1BNATNFRSF1B
Entrez,  Source
tumor necrosis factor receptor superfamily, member 1B3423.6280.2432Yes
18PTGER2NAPTGER2
Entrez,  Source
prostaglandin E receptor 2 (subtype EP2), 53kDa3893.4550.2530Yes
19RTP4NARTP4
Entrez,  Source
receptor transporter protein 43923.4500.2653Yes
20CD55NACD55
Entrez,  Source
CD55 molecule, decay accelerating factor for complement (Cromer blood group)4313.3620.2752Yes
21CYBBNACYBB
Entrez,  Source
cytochrome b-245, beta polypeptide (chronic granulomatous disease)5553.0590.2793Yes
22IL18NAIL18
Entrez,  Source
interleukin 18 (interferon-gamma-inducing factor)5643.0300.2897Yes
23FPR1NAFPR1
Entrez,  Source
formyl peptide receptor 15653.0290.3006Yes
24CD14NACD14
Entrez,  Source
CD14 molecule5712.9980.3111Yes
25CCRL2NACCRL2
Entrez,  Source
chemokine (C-C motif) receptor-like 26112.9250.3194Yes
26SERPINE1NASERPINE1
Entrez,  Source
serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 16202.9100.3294Yes
27SGMS2NA6552.8330.3376Yes
28FZD5NAFZD5
Entrez,  Source
frizzled homolog 5 (Drosophila)6812.7560.3461Yes
29SLC7A2NASLC7A2
Entrez,  Source
solute carrier family 7 (cationic amino acid transporter, y+ system), member 27232.6730.3534Yes
30IFITM1NAIFITM1
Entrez,  Source
interferon induced transmembrane protein 1 (9-27)7482.6390.3615Yes
31TNFSF9NATNFSF9
Entrez,  Source
tumor necrosis factor (ligand) superfamily, member 97492.6390.3710Yes
32RGS16NARGS16
Entrez,  Source
regulator of G-protein signalling 167542.6300.3802Yes
33METNAMET
Entrez,  Source
met proto-oncogene (hepatocyte growth factor receptor)8712.4450.3825Yes
34F3NAF3
Entrez,  Source
coagulation factor III (thromboplastin, tissue factor)8762.4380.3910Yes
35CDKN1ANACDKN1A
Entrez,  Source
cyclin-dependent kinase inhibitor 1A (p21, Cip1)8882.4130.3990Yes
36AHRNAAHR
Entrez,  Source
aryl hydrocarbon receptor10012.2890.4009Yes
37NAMPTNA10472.2320.4064Yes
38IRF7NAIRF7
Entrez,  Source
interferon regulatory factor 710582.2150.4138Yes
39OPRK1NAOPRK1
Entrez,  Source
opioid receptor, kappa 110672.2040.4213Yes
40CXCL11NACXCL11
Entrez,  Source
chemokine (C-X-C motif) ligand 1111802.0560.4224Yes
41MMP14NAMMP14
Entrez,  Source
matrix metallopeptidase 14 (membrane-inserted)12022.0380.4285Yes
42KIF1BNAKIF1B
Entrez,  Source
kinesin family member 1B12302.0100.4342Yes
43ICAM4NAICAM4
Entrez,  Source
intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)13361.8980.4351Yes
44TLR1NATLR1
Entrez,  Source
toll-like receptor 114621.7960.4345Yes
45TAPBPNATAPBP
Entrez,  Source
TAP binding protein (tapasin)15261.7390.4372Yes
46GPR132NAGPR132
Entrez,  Source
G protein-coupled receptor 13216211.6640.4379Yes
47RNF144BNA16631.6380.4415Yes
48CXCL10NACXCL10
Entrez,  Source
chemokine (C-X-C motif) ligand 1016721.6340.4469Yes
49ADORA2BNAADORA2B
Entrez,  Source
adenosine A2b receptor16851.6150.4520Yes
50TPBGNATPBG
Entrez,  Source
trophoblast glycoprotein17231.5840.4556Yes
51ACVR2ANAACVR2A
Entrez,  Source
activin A receptor, type IIA18341.5080.4548Yes
52IL7RNAIL7R
Entrez,  Source
interleukin 7 receptor19391.4520.4542Yes
53ICAM1NAICAM1
Entrez,  Source
intercellular adhesion molecule 1 (CD54), human rhinovirus receptor19971.4220.4561Yes
54ATP2B1NAATP2B1
Entrez,  Source
ATPase, Ca++ transporting, plasma membrane 120211.4110.4599Yes
55BDKRB1NABDKRB1
Entrez,  Source
bradykinin receptor B120281.4090.4646Yes
56SLC28A2NASLC28A2
Entrez,  Source
solute carrier family 28 (sodium-coupled nucleoside transporter), member 221011.3620.4654Yes
57SLC31A2NASLC31A2
Entrez,  Source
solute carrier family 31 (copper transporters), member 221171.3490.4694Yes
58SCN1BNASCN1B
Entrez,  Source
sodium channel, voltage-gated, type I, beta22641.2730.4658Yes
59SEMA4DNASEMA4D
Entrez,  Source
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D22771.2660.4696Yes
60ITGA5NAITGA5
Entrez,  Source
integrin, alpha 5 (fibronectin receptor, alpha polypeptide)22931.2580.4733Yes
61PSEN1NAPSEN1
Entrez,  Source
presenilin 1 (Alzheimer disease 3)24261.2030.4702Yes
62IRF1NAIRF1
Entrez,  Source
interferon regulatory factor 124391.1970.4738Yes
63EIF2AK2NAEIF2AK2
Entrez,  Source
eukaryotic translation initiation factor 2-alpha kinase 224731.1870.4762Yes
64HPNNAHPN
Entrez,  Source
hepsin (transmembrane protease, serine 1)25981.1370.4733Yes
65P2RX7NAP2RX7
Entrez,  Source
purinergic receptor P2X, ligand-gated ion channel, 727411.0820.4692Yes
66PVRNAPVR
Entrez,  Source
poliovirus receptor27491.0790.4727Yes
67PROK2NAPROK2
Entrez,  Source
prokineticin 227591.0750.4761Yes
68TACR1NATACR1
Entrez,  Source
tachykinin receptor 128831.0340.4729Yes
69CXCR6NACXCR6
Entrez,  Source
chemokine (C-X-C motif) receptor 629601.0020.4722Yes
70RASGRP1NARASGRP1
Entrez,  Source
RAS guanyl releasing protein 1 (calcium and DAG-regulated)30190.9860.4725Yes
71IL18RAPNAIL18RAP
Entrez,  Source
interleukin 18 receptor accessory protein30670.9710.4733Yes
72CALCRLNACALCRL
Entrez,  Source
calcitonin receptor-like31560.9460.4717Yes
73C5AR1NAC5AR1
Entrez,  Source
complement component 5a receptor 131840.9430.4736Yes
74OSMRNAOSMR
Entrez,  Source
oncostatin M receptor31920.9410.4766Yes
75TIMP1NATIMP1
Entrez,  Source
TIMP metallopeptidase inhibitor 132840.9170.4748No
76AXLNAAXL
Entrez,  Source
AXL receptor tyrosine kinase33990.8820.4715No
77HBEGFNAHBEGF
Entrez,  Source
heparin-binding EGF-like growth factor34510.8700.4718No
78CCL17NACCL17
Entrez,  Source
chemokine (C-C motif) ligand 1736680.8330.4626No
79SLC31A1NASLC31A1
Entrez,  Source
solute carrier family 31 (copper transporters), member 138350.8010.4562No
80IL15RANAIL15RA
Entrez,  Source
interleukin 15 receptor, alpha39170.7820.4544No
81CHST2NACHST2
Entrez,  Source
carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 239200.7800.4571No
82TLR3NATLR3
Entrez,  Source
toll-like receptor 339450.7740.4585No
83CX3CL1NACX3CL1
Entrez,  Source
chemokine (C-X3-C motif) ligand 141370.7320.4504No
84IFNAR1NAIFNAR1
Entrez,  Source
interferon (alpha, beta and omega) receptor 142510.7110.4466No
85ROS1NAROS1
Entrez,  Source
v-ros UR2 sarcoma virus oncogene homolog 1 (avian)44720.6620.4366No
86NFKBIANANFKBIA
Entrez,  Source
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha44750.6620.4389No
87CCL5NACCL5
Entrez,  Source
chemokine (C-C motif) ligand 546740.6240.4300No
88FFAR2NAFFAR2
Entrez,  Source
free fatty acid receptor 248640.5820.4214No
89ATP2A2NAATP2A2
Entrez,  Source
ATPase, Ca++ transporting, cardiac muscle, slow twitch 249050.5760.4212No
90EMP3NAEMP3
Entrez,  Source
epithelial membrane protein 352170.5150.4056No
91GPC3NAGPC3
Entrez,  Source
glypican 352610.5080.4050No
92ADMNAADM
Entrez,  Source
adrenomedullin52920.5020.4051No
93CCL20NACCL20
Entrez,  Source
chemokine (C-C motif) ligand 2054700.4820.3969No
94IL18R1NAIL18R1
Entrez,  Source
interleukin 18 receptor 155700.4650.3930No
95LCP2NALCP2
Entrez,  Source
lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)55820.4630.3940No
96GP1BANAGP1BA
Entrez,  Source
glycoprotein Ib (platelet), alpha polypeptide57170.4410.3881No
97GABBR1NAGABBR1
Entrez,  Source
gamma-aminobutyric acid (GABA) B receptor, 157740.4340.3865No
98TACR3NATACR3
Entrez,  Source
tachykinin receptor 358650.4280.3830No
99PDPNNAPDPN
Entrez,  Source
podoplanin59400.4230.3803No
100NPFFR2NANPFFR2
Entrez,  Source
neuropeptide FF receptor 260720.4180.3744No
101KCNMB2NAKCNMB2
Entrez,  Source
potassium large conductance calcium-activated channel, subfamily M, beta member 261420.4150.3721No
102ADRM1NAADRM1
Entrez,  Source
adhesion regulating molecule 162080.4140.3699No
103GPR183NA62730.4020.3677No
104LTANALTA
Entrez,  Source
lymphotoxin alpha (TNF superfamily, member 1)64790.3700.3575No
105MARCONAMARCO
Entrez,  Source
macrophage receptor with collagenous structure66360.3440.3500No
106AQP9NAAQP9
Entrez,  Source
aquaporin 967650.3260.3440No
107CCR7NACCR7
Entrez,  Source
chemokine (C-C motif) receptor 767670.3260.3451No
108RHOGNARHOG
Entrez,  Source
ras homolog gene family, member G (rho G)69470.2980.3361No
109CLEC5ANACLEC5A
Entrez,  Source
C-type lectin domain family 5, member A70050.2890.3339No
110NMUR1NANMUR1
Entrez,  Source
neuromedin U receptor 170510.2810.3324No
111VIPNAVIP
Entrez,  Source
vasoactive intestinal peptide70970.2750.3308No
112CCL22NACCL22
Entrez,  Source
chemokine (C-C motif) ligand 2271890.2590.3266No
113SELENASELE
Entrez,  Source
selectin E (endothelial adhesion molecule 1)72150.2550.3262No
114SLC11A2NASLC11A2
Entrez,  Source
solute carrier family 11 (proton-coupled divalent metal ion transporters), member 275550.2040.3078No
115CD69NACD69
Entrez,  Source
CD69 molecule75690.2020.3078No
116PLAURNAPLAUR
Entrez,  Source
plasminogen activator, urokinase receptor76760.1880.3025No
117KLF6NAKLF6
Entrez,  Source
Kruppel-like factor 676950.1850.3022No
118NOD2NA78070.1700.2965No
119HIF1ANAHIF1A
Entrez,  Source
hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)81110.1320.2800No
120APLNRNA83430.1020.2673No
121ATP2C1NAATP2C1
Entrez,  Source
ATPase, Ca++ transporting, type 2C, member 183990.0940.2646No
122MEFVNAMEFV
Entrez,  Source
Mediterranean fever85070.0780.2588No
123PTAFRNAPTAFR
Entrez,  Source
platelet-activating factor receptor85540.0730.2565No
124IL10NAIL10
Entrez,  Source
interleukin 1086290.0620.2526No
125MEP1ANAMEP1A
Entrez,  Source
meprin A, alpha (PABA peptide hydrolase)86830.0560.2498No
126NMINANMI
Entrez,  Source
N-myc (and STAT) interactor87540.0440.2460No
127OSMNAOSM
Entrez,  Source
oncostatin M87770.0420.2449No
128KCNA3NAKCNA3
Entrez,  Source
potassium voltage-gated channel, shaker-related subfamily, member 391850.0000.2220No
129CCL7NACCL7
Entrez,  Source
chemokine (C-C motif) ligand 793920.0000.2105No
130CD70NA93950.0000.2103No
131RELANARELA
Entrez,  Source
v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65 (avian)9422-0.0030.2089No
132IFNGR2NAIFNGR2
Entrez,  Source
interferon gamma receptor 2 (interferon gamma transducer 1)9453-0.0070.2072No
133ABI1NAABI1
Entrez,  Source
abl-interactor 19521-0.0190.2035No
134TNFRSF9NATNFRSF9
Entrez,  Source
tumor necrosis factor receptor superfamily, member 99604-0.0300.1990No
135GNA15NAGNA15
Entrez,  Source
guanine nucleotide binding protein (G protein), alpha 15 (Gq class)9758-0.0470.1906No
136HRH1NAHRH1
Entrez,  Source
histamine receptor H19866-0.0590.1848No
137PTGER4NAPTGER4
Entrez,  Source
prostaglandin E receptor 4 (subtype EP4)9872-0.0610.1847No
138RGS1NARGS1
Entrez,  Source
regulator of G-protein signalling 19913-0.0640.1827No
139SLC1A2NASLC1A2
Entrez,  Source
solute carrier family 1 (glial high affinity glutamate transporter), member 29937-0.0650.1816No
140CXCL9NACXCL9
Entrez,  Source
chemokine (C-X-C motif) ligand 910000-0.0700.1784No
141IL10RANAIL10RA
Entrez,  Source
interleukin 10 receptor, alpha10061-0.0740.1753No
142ACVR1BNAACVR1B
Entrez,  Source
activin A receptor, type IB10124-0.0790.1721No
143LYNNALYN
Entrez,  Source
v-yes-1 Yamaguchi sarcoma viral related oncogene homolog10237-0.0910.1661No
144KCNJ2NAKCNJ2
Entrez,  Source
potassium inwardly-rectifying channel, subfamily J, member 210277-0.0960.1643No
145TNFAIP6NATNFAIP6
Entrez,  Source
tumor necrosis factor, alpha-induced protein 610779-0.1660.1367No
146MXD1NAMXD1
Entrez,  Source
MAX dimerization protein 110789-0.1680.1368No
147GNAI3NAGNAI3
Entrez,  Source
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 310843-0.1760.1344No
148CCL24NACCL24
Entrez,  Source
chemokine (C-C motif) ligand 2410853-0.1770.1346No
149IL12BNAIL12B
Entrez,  Source
interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)11133-0.2160.1196No
150CD82NACD82
Entrez,  Source
CD82 molecule11143-0.2180.1199No
151PIK3R5NAPIK3R5
Entrez,  Source
phosphoinositide-3-kinase, regulatory subunit 5, p10111218-0.2280.1166No
152DCBLD2NADCBLD2
Entrez,  Source
discoidin, CUB and LCCL domain containing 211289-0.2380.1135No
153STAB1NASTAB1
Entrez,  Source
stabilin 111361-0.2480.1104No
154SRINASRI
Entrez,  Source
sorcin11416-0.2570.1083No
155ITGB8NAITGB8
Entrez,  Source
integrin, beta 811443-0.2610.1077No
156IRAK2NAIRAK2
Entrez,  Source
interleukin-1 receptor-associated kinase 211665-0.2910.0964No
157OLR1NAOLR1
Entrez,  Source
oxidised low density lipoprotein (lectin-like) receptor 112060-0.3470.0754No
158BEST1NA12065-0.3480.0765No
159RIPK2NARIPK2
Entrez,  Source
receptor-interacting serine-threonine kinase 212241-0.3740.0680No
160LDLRNALDLR
Entrez,  Source
low density lipoprotein receptor (familial hypercholesterolemia)12407-0.4000.0601No
161SLAMF1NASLAMF1
Entrez,  Source
signaling lymphocytic activation molecule family member 112435-0.4070.0601No
162CSF3RNACSF3R
Entrez,  Source
colony stimulating factor 3 receptor (granulocyte)12554-0.4110.0549No
163IL15NAIL15
Entrez,  Source
interleukin 1512600-0.4160.0539No
164RAF1NARAF1
Entrez,  Source
v-raf-1 murine leukemia viral oncogene homolog 112700-0.4340.0498No
165CXCL6NACXCL6
Entrez,  Source
chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2)12913-0.4510.0395No
166GCH1NAGCH1
Entrez,  Source
GTP cyclohydrolase 1 (dopa-responsive dystonia)13276-0.5010.0210No
167IL4RNAIL4R
Entrez,  Source
interleukin 4 receptor13336-0.5080.0195No
168PDE4BNAPDE4B
Entrez,  Source
phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)13627-0.5630.0052No
169ITGB3NAITGB3
Entrez,  Source
integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)13638-0.5650.0067No
170CCL2NACCL2
Entrez,  Source
chemokine (C-C motif) ligand 213859-0.606-0.0035No
171EDN1NAEDN1
Entrez,  Source
endothelin 114208-0.684-0.0207No
172NFKB1NANFKB1
Entrez,  Source
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)14554-0.768-0.0373No
173MYCNAMYC
Entrez,  Source
v-myc myelocytomatosis viral oncogene homolog (avian)15055-0.881-0.0623No
174LCKNALCK
Entrez,  Source
lymphocyte-specific protein tyrosine kinase15773-1.086-0.0987No
175MSR1NAMSR1
Entrez,  Source
macrophage scavenger receptor 115923-1.146-0.1030No
176NLRP3NA16002-1.177-0.1031No
177C3AR1NAC3AR1
Entrez,  Source
complement component 3a receptor 116041-1.192-0.1010No
178LY6ENALY6E
Entrez,  Source
lymphocyte antigen 6 complex, locus E16427-1.376-0.1177No
179LIFNALIF
Entrez,  Source
leukemia inhibitory factor (cholinergic differentiation factor)16734-1.553-0.1294No
180SLC7A1NASLC7A1
Entrez,  Source
solute carrier family 7 (cationic amino acid transporter, y+ system), member 116964-1.737-0.1360No
181P2RX4NAP2RX4
Entrez,  Source
purinergic receptor P2X, ligand-gated ion channel, 417097-1.871-0.1367No
182SPHK1NASPHK1
Entrez,  Source
sphingosine kinase 117256-2.092-0.1381No
183IL1BNAIL1B
Entrez,  Source
interleukin 1, beta17373-2.282-0.1364No
184PTPRENAPTPRE
Entrez,  Source
protein tyrosine phosphatase, receptor type, E17374-2.287-0.1282No
185IL2RBNAIL2RB
Entrez,  Source
interleukin 2 receptor, beta17450-2.412-0.1238No
186CD40NACD40
Entrez,  Source
CD40 molecule, TNF receptor superfamily member 517453-2.414-0.1152No
187CD48NACD48
Entrez,  Source
CD48 molecule17597-2.740-0.1134No
188HAS2NAHAS2
Entrez,  Source
hyaluronan synthase 217666-2.972-0.1066No
189ABCA1NAABCA1
Entrez,  Source
ATP-binding cassette, sub-family A (ABC1), member 117737-3.297-0.0987No
190PTGIRNAPTGIR
Entrez,  Source
prostaglandin I2 (prostacyclin) receptor (IP)17762-3.412-0.0878No
191SCARF1NASCARF1
Entrez,  Source
scavenger receptor class F, member 117771-3.457-0.0758No
192IL1ANAIL1A
Entrez,  Source
interleukin 1, alpha17815-3.646-0.0652No
193BTG2NABTG2
Entrez,  Source
BTG family, member 217816-3.647-0.0521No
194TNFSF15NATNFSF15
Entrez,  Source
tumor necrosis factor (ligand) superfamily, member 1517870-4.070-0.0405No
195INHBANAINHBA
Entrez,  Source
inhibin, beta A (activin A, activin AB alpha polypeptide)17879-4.121-0.0261No
196EBI3NAEBI3
Entrez,  Source
Epstein-Barr virus induced gene 317898-4.265-0.0118No
197CSF3NACSF3
Entrez,  Source
colony stimulating factor 3 (granulocyte)17920-4.4700.0030No
Table: GSEA details [plain text format]



Fig 2: HALLMARK_INFLAMMATORY_RESPONSE   
Blue-Pink O' Gram in the Space of the Analyzed GeneSet



Fig 3: HALLMARK_INFLAMMATORY_RESPONSE: Random ES distribution   
Gene set null distribution of ES for HALLMARK_INFLAMMATORY_RESPONSE