Load image

Description

Load image into MIA workspace. This module can be configured to import images from a variety of locations (selected using the "Import mode" control).

Parameters

Core image loading controls
- Type: ParamSeparatorP
Output image
- Description: Name assigned to the image.
- Type: OutputImageP
Import mode
- Description: File reader mode to use.
  - "Current file" (default option) will import the current root-file for the workspace.
  - "From ImageJ" will load the active image fromm ImageJ.
  - "Image sequence" will use the root-file for the workspace as the basis for loading a series of images with numbered suffixes.
  - "Matching format" will load the image matching a filename based on the root-file for the workspace and a series of rules.
  - "Specific file" will load the image at a specific location.
- Type: ChoiceP
- Default value: Current file
- Choices:
  - Current file
  - From ImageJ
  - Image sequence
  - Matching format
  - Specific file
Use ImageJ reader
- Description: Use the stock ImageJ file reader, rather than the default BioFormats reader.
- Type: BooleanP
- Default value: false
Number of zeroes
- Description: Number of digits in image sequence suffix.
- Type: IntegerP
- Default value: 4
Starting index
- Description: First number in sequence to load.
- Type: IntegerP
- Default value: 0
Frame interval
- Description: Frame interval to use for loading.
- Type: IntegerP
- Default value: 1
Limit frames
- Description: When "true" this will load a pre-determined number of frames. When "false" it will load all the available images.
- Type: BooleanP
- Default value: false
Final index
- Description: Final number in sequence to load.
- Type: IntegerP
- Default value: 1
Name format
- Type: ChoiceP
- Default value: Huygens
- Choices:
  - Huygens
  - Incucyte short filename
  - Yokogowa
  - Input filename with prefix
  - Input filename with suffix
Comment
- Type: StringP
Prefix
- Type: StringP
Suffix
- Type: StringP
Extension
- Type: StringP
Include series number
- Type: BooleanP
- Default value: true
File path
- Type: FilePathP
Dimension ranges and cropping
- Type: ParamSeparatorP
Channels
- Type: StringP
- Default value: 1-end
Slices
- Type: StringP
- Default value: 1-end
Frames
- Type: StringP
- Default value: 1-end
Channel
- Type: IntegerP
- Default value: 1
Load 3D stacks as
- Type: ChoiceP
- Default value: Z-Stack
- Choices:
  - Timeseries
  - Z-Stack
Crop mode
- Type: ChoiceP
- Default value: None
- Choices:
  - None
  - Fixed
  - From reference
Reference image
- Type: InputImageP
Left coordinate
- Type: IntegerP
- Default value: 0
Top coordinate
- Type: IntegerP
- Default value: 0
Width
- Type: IntegerP
- Default value: 512
Height
- Type: IntegerP
- Default value: 512
Spatial and intensity calibration
- Type: ParamSeparatorP
Set manual spatial calibration
- Type: BooleanP
- Default value: false
XY calibration (dist/px)
- Type: DoubleP
- Default value: 1.0
Z calibration (dist/px)
- Type: DoubleP
- Default value: 1.0
Force bit depth
- Type: BooleanP
- Default value: false
Output bit depth
- Type: ChoiceP
- Default value: 8
- Choices:
  - 8
  - 16
  - 32
Minimum input intensity
- Type: DoubleP
- Default value: 0.0
Maximum input intensity
- Type: DoubleP
- Default value: 1.0