An 8-year old female spayed beagle presented with a complaint of intermittent left front limb lameness of 4-day duration. Obvious lameness was not appreciated on physical examination; however, considerable pain was apparent upon palpation of the humeroscapular joint. The remainder of the physical examination was normal with the exception of dental calculus and a body condition score of 7/9. Radiographic study under sedation was recommended, but the client opted for symptomatic treatment of pain and bed rest. Tramadol was prescribed at 4.6 mg/kg every 12 hours for 14 days. The patient returned 8 days later for persistent lameness. Per the client, Tramadol was given at the recommended dosage for three consecutive days, but was discontinued due to lack of improvement. Pain was again elicited on palpation of the left humeroscapular joint, as well as with its extension and flexion. Radiographs with consult were approved at this visit, which revealed multiple small regions of radiolucency in the distal aspect of the spine of the left scapula, including the acromion process (). Radiographic consult by a board-certified radiologist stated a concern for an aggressive lytic lesion; possibly representing a neoplastic process such as a primary mesenchymal tumor or a round cell tumor. The radiolucencies were subtle, so the possibility that it represented a superimposition artifact could not be ruled out. The radiologist recommended an ultrasound evaluation of the affected scapula. If a cortical irregularity were to be identified on ultrasound, bones aspirates should be obtained for cytologic evaluation. Alternatively, follow-up radiographs were suggested to be evaluated in 3–5 week to reassess the appearance of the left scapula. Carprofen was prescribed at 2.2 mg/kg twice daily for 7 days to evaluate the patient’s response while awaiting the client’s decision. The client called 2 weeks after the last appointment stating she had taken the patient to a different veterinary clinic where a second set of radiographs were taken, which revealed a more pronounced radiolucency in the left scapula (images not available due to analog radiographs being taken at a different clinic). Due to the severity of the progressing limb pain, the client opted to perform bone biopsy under general anesthesia versus ultrasound-guided cytology, as the cost of the latter at a specialty clinic was considerably more than the former at the general practice. A fentanyl patch of 50 mcg/hour was prescribed and applied prior to the bone biopsy procedure performed the following day. Blood work performed prior to general anesthesia showed a slight increase in hematocrit of 55.21% [reference range (RR) 37.0%–55.0%] and chemistry showed a mild to moderate increase in the levels of alkaline phosphatase 405 U/l (RR 23.0–212.0 U/l) and a slight decrease in the levels of globulin 2.2 g/dl (RR 2.5–4.5 g/dl). Electrolytes levels were within the reference range. A bone biopsy was performed with J-type trephine to acquire three samples from the proximal scapula, which were submitted for histopathology. Anesthesia and recovery for the procedure were uneventful. Gabapentin at 7 mg/kg twice daily and Carprofen at 2.2 mg/kg twice daily were prescribed in addition to the Fentanyl patch (changed every 72 hours) for supplemental pain relief. The owner reported that the patient’s pain was well controlled with the combination of all three drugs. Laboratory results returned a few days later, and the microscopic description of the bone biopsy was reported as follows: infiltrating, what appears to be reactive woven bone and skeletal muscle, is a mass composed of polygonal cells arranged in cords and clusters. Cells have round nuclei, finely stippled chromatin, inconspicuous nucleoli, a moderate amount of amphophilic cytoplasm, and distinct cell borders. Anisocytosis and anisokaryosis are mild to moderate. Mitoses are occasionally observed. Cells appear to occasionally surround a lumen filled with lightly basophilic material. Osteoclasts are occasionally observed within Howship’s lacunae. The biopsy results were reviewed by three board-certified pathologists and concluded to be a metastatic carcinoma. Primary carcinoma was suggested to be originating from mammary gland, urinary bladder, or prostate tissue. Ten days following the bone biopsy procedure, the client consulted with a board-certified oncologist. The patient had lost 1.76 kg from the time of initial presentation (approximately 5 weeks), but besides left front limb lameness, no other significant abnormalities were noted on physical examination. An abdominal ultrasound was performed on the same day by the oncology specialist, which revealed a mass in the trigone region of the urinary bladder. The remainder of the abdominal ultrasound was normal. The bladder mass was aspirated percutaneously and sent out for the cytologic evaluation. With cytology results pending, the subsequent options for pain control and improved quality of life were offered: 1) left front limb amputation, 2) radiation, or 3) bisphosphonate therapy plus continued oral pain medication in combination with a fentanyl patch. Chemotherapy was also discussed, pending cytology results. Due to the severity of the front limb pain, the owner opted for limb amputation. Carprofen was replaced with Piroxicam at 0.26 mg/kg/day, and Gabapentin and Fentanyl patch continued as previously prescribed. Amputation was scheduled to be performed the following day as long as there was no radiographic evidence of metastasis to the unaffected limbs. Radiographs of the hind limbs were taken and included lumbar vertebrae 6 and 7, pelvis, femur, tibia, and proximal metatarsal bones. Non-affected right front limb radiographs included cervical vertebrae 2–6, thoracic vertebrae 1–11, scapula, humerus, radius, and ulna. All included bones were reviewed and deemed free of obvious metastatic disease on radiographs. Blood work prior to general anesthesia for limb amputation was again performed and revealed similar abnormalities as the previous blood work. Immediately prior to initiating surgery, the oncologist called with the cytology report of the bladder mass. The microscopic findings of the needle aspirate of the urinary bladder were reported as follows: one smear is large and densely cellular with abnormal epithelial cells, which are spread in a monolayer. The epithelial cells are rounded and slightly polygonal and have round nuclei, fine chromatin, multiple nucleoli, blue cytoplasm, and a higher nuclear to cytoplasm ratio than normal epithelial cells. There is moderate variation in sizes of cells, nuclei, and nucleoli, including some large cells with large single nucleus and cells with more than on nucleus. Mitotic cells are easily found. A few cells contain round, granular, pink inclusions. One small smear also contains abnormal cells. Additional comments communicated that the location of the mass and morphology of the neoplastic cells were consistent with transitional cell carcinoma. The client was informed of the results by the oncology specialist and opted to proceed with the planned front left limb amputation. Chemotherapy was scheduled with the oncology specialist 2 weeks post-surgery. No complications occurred during surgery or recovery. The patient was discharged on the same day with a new Fentanyl patch (q72h) in place. The same dosing for Gabapentin and Piroxicam were continued. Fourteen days after surgery, the patient presented to the oncology specialist for suture removal and initiation of Mitoxantrone for TCC. The owner reported that the patient had been progressively weaker since the limb amputation and had been experiencing episodes of pale mucous membranes. On physical examination, the patient was down, mucous membranes were pale, and had a painful abdomen. A work-up consisting of a complete blood cell count (CBC), abdominal ultrasound and abdominal radiographs were performed prior to chemotherapy administration. CBC showed a hematocrit of 14% (RR 37.0%–55.0%), white cell count of 29,000 μl (RR 6,000–17,000 μl), and neutrophilia at 13,000 μl (RR 2,000–10,600 μl). The severe anemia was suspected to be due to a gastrointestinal ulcer induced by Piroxicam administration. The need for a blood transfusion was discussed, as well as a repeat of an abdominal ultrasound and radiographs, if moving forward with chemotherapy. Abdominal ultrasound revealed extreme thickening of the stomach, no overt free fluid, and enlargement of the urinary bladder mass. Abdominal radiographs revealed no free gas presents; however, a lytic lesion on the L5 vertebra with a secondary pathologic fracture was detected. Although the affected vertebra was not biopsied, it was suspected to be another distant metastatic site from the TCC. After discussing the aggressive progression of metastatic disease resulting in a pathologic fracture of the L5 vertebrae, giving the patient an extremely poor quality of life, the owner opted for humane euthanasia.