A 27-year-old, previously healthy female was hospitalized because of swelling and pain in her vulva. The mass, which was located on the left labia majora, was red, warm, and tender. The patient had not received any treatment before visiting the doctor. On admission, the patient’s blood pressure was 118/68 mmHg; heart rate, 95 beats/min; and body temperature, 38℃. Laboratory findings suggested inflammation (C-reactive protein, 38.90 mg/L) and hyperleukocytosis (14.1 × 109/L). The pH of leucorrhea was elevated (pH, 4.8) and the amine test was positive. Cervical secretions tested negative for human papillomavirus (HPV). Serologic tests for syphilis and human immunodeficiency virus were both negative. Ultrasonography showed a cystic mass (measuring approximately 5.6 × 3.6 cm) in the subcutaneous area of the vulva with poor ultrasonic echo. The condition was diagnosed as Bartholin’s gland cyst for which we decided to perform surgical neostomy and cystectomy. The patient was placed in the lithotomy position after anesthesia, and the vaginal mucosa of the inner labia majora was dissected. The purulent fluid in the cyst was extracted for microbiological examination and then washed repeatedly with povidone-iodine. The stoma was formed by discontinuous suturing between the cyst wall and vaginal mucosa. The patient was administered clindamycin phosphate injection and supportive treatments postoperatively and she recovered after 6 days.