FastQCFastQC Report
Fri 21 Jul 2023
Syr2_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr2_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15616969
Sequences flagged as poor quality0
Sequence length20-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT5710023.6562920756261987No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA2652161.6982552760398No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA1612721.032671576667662No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC1415090.9061233328951347No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA1276260.8172264413152129No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT1247740.7989642548435615No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA950460.608607214370471No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC868990.556439601051907No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG790720.5063210409138931No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC735990.4712758282352997No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG728760.46664624870549465No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG691230.4426146968723572No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG658020.4213493668329623No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA626040.4008716416098412No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA601330.38504910908128204No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC587630.37627660015205255No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA571240.36578160589292325No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC570240.36514127677400143No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG566540.3627720590339905No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC541890.3469879462525667No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA522460.33454635147191497No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG506590.32438432835462505No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA467510.29936026638715874No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT447450.2865152642615862No Hit
GTCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCC381110.24403583051231004No Hit
TAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCA366650.23477667145269995No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT351480.22506287871865532No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG347170.2223030602161021No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC346570.22191886274474898No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA344370.22051013868312092No Hit
GGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCT333420.21349853483092654No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC328500.210348115565831No Hit
GGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTC326970.20936841201388054No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG318230.20377193551450348No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC313840.2009608906824365No Hit
CTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGT289160.185157568027445No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA286370.18337104978565302No Hit
TAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAG263580.16877794916542385No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT251320.16092751416744183No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC250550.160434460745872No Hit
GCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAA246010.15752736654596677No Hit
GGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCC236460.151412223460263No Hit
TCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTA234860.150387696869988No Hit
CTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTT228330.14620634772342828No Hit
GCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA220810.14139107274913587No Hit
CCGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAG213220.13653097473651898No Hit
ACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA209380.13407211091985902No Hit
AGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACC208130.1332716995212067No Hit
CAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAA201570.12907114050107932No Hit
CATAACTTGGCGTATTGGTGTGAACGACCAATTTGCCTTCAGGTACTTTC197200.1262729022513908No Hit
GTGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGA196990.1261384331364172No Hit
GTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAGACTTCGC194480.12453120704792332No Hit
GAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAAC190950.12227084525812916No Hit
TGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTA190400.12191866424272213No Hit
GTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAAAG188430.12065721587844608No Hit
GTCGTACTCCATTTTTGACGCCCTAGCGAAAGCTAGGTGGGTGTCTTGTC187150.11983759460622609No Hit
GTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAAC184630.11822396522654299No Hit
TGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAA181900.11647586673188633No Hit
TATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTA177530.1136776284821978No Hit
TGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCT176350.11292204012187No Hit
GTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTC175960.11267231176549047No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT171470.10979723402153131No Hit
GGAGGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTT170710.10931058389115071No Hit
TTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGT170240.10900962920525745No Hit
GGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGA169130.10829886388325417No Hit
CGGCGATTAAAAACATTTTTGATATTTACGACCCTGAAATCATTGCCGTT164360.10524449398599689No Hit
CCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGGC163580.10474503727323785No Hit
CGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGA161550.10344516916182646No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA160020.10246546560987602No Hit
GTTACGGGTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAG157240.10068535065927327No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)