FastQCFastQC Report
Fri 21 Jul 2023
Syr1_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr1_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14251136
Sequences flagged as poor quality0
Sequence length20-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT6411984.4992764085613945No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA4762593.34190200696983No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA2153661.5112198774890646No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA2028941.4237040471720992No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC1935471.358116293325669No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT1866351.30961489666508No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC1345330.9440159717793726No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG1162920.8160191580516809No Hit
GTCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCC1058520.7427618401789162No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA1050100.7368535392546952No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG907990.637135172943406No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC864730.6067796981237145No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC837990.5880162816494067No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG835920.5865637658639985No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG739850.5191515960552198No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA729600.5119591869728841No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC674670.4734148912760358No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA650410.4563916869504298No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA627950.4406315398295266No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC591290.4149072747604121No Hit
GTCGTACTCCATTTTTGACGCCCTAGCGAAAGCTAGGTGGGTGTCTTGTC588430.4129004172018287No Hit
TAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCA579670.40675353880560816No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG573370.40233283858914826No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA552440.38764629009224244No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC506300.35526992374502636No Hit
GGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCT496620.34847748277751334No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC478210.3355592143671915No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT472460.331524448296613No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT459140.322177824981812No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA430300.30194084176868424No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC419010.29401866630140927No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA412230.28926115083036186No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT411550.288783995886363No Hit
CTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGT401200.2815214169593217No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC396060.277914686941448No Hit
CTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTT373520.26209840394478023No Hit
GCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA371610.2607581599109011No Hit
CCGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAG357890.25113085721727724No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG345620.24252101727188624No Hit
GGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCC335530.23544087994107976No Hit
CCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTC331710.23276039187332154No Hit
GTTGAATTCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGGAAAGT329440.23116753639850185No Hit
GGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTC324050.22738538176886391No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG322360.22619951139333733No Hit
TAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAG313870.2202420915778223No Hit
AAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTGTG312590.21934391756558916No Hit
CAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCG306040.21474779273736494No Hit
GCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTC293290.2058011375373865No Hit
CGTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAG290610.20392058569927338No Hit
GTTCGTTGCCAGATGTTAGGCCTCATTATATCAGTGGAAAACAGAAGTCA289150.20289610596656996No Hit
GGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGA287300.20159796383951425No Hit
CGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGA282520.19824384526258118No Hit
CGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCG280120.19655976898964406No Hit
GTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTC276600.19408979045600294No Hit
GTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTG264580.1856553751223762No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT255100.17900327384427459No Hit
ACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA247290.17352300897275838No Hit
GTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAAC246890.17324232959393554No Hit
TGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCT241730.16962156560712072No Hit
AGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACC236420.16589554685324734No Hit
GACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACT235680.16537629000242507No Hit
GCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAA228830.1605696556400837No Hit
TGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTA224520.15754533533326745No Hit
CGCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA220930.15502623790833236No Hit
TCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGGAAAGTGGCGTTG220540.15475257551398008No Hit
GCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGC214870.15077394531916613No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG213030.14948282017658102No Hit
GCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGG210260.14753911547823276No Hit
TCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCT208920.1465988395591762No Hit
CTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTCAACC208380.14621992239776535No Hit
GCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGC206920.14519544266506193No Hit
GAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAAC206610.1449779161464742No Hit
TATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTA205680.1443253365907111No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA196650.13798899961378516No Hit
TAGACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGG196540.1379118127846089No Hit
ACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTT191980.13471206786602835No Hit
AGACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGT191080.13408053926367694No Hit
ATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTCTGGC190240.13349111256814897No Hit
GTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAGACTTCGC189880.1332385011272084No Hit
TCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTA188040.13194737598462325No Hit
GGAGGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTT184900.12974404286086386No Hit
GAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTC181480.12734423417192847No Hit
GAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAAT178400.1251830029549925No Hit
CAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCT177750.12472689896440538No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA177280.1243971006942885No Hit
CTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGATCCTCCGGCA176840.12408835337758338No Hit
TTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGT175730.12330946810134996No Hit
CTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTCAACCCTGCT174410.12238322615123454No Hit
GACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTT173170.12151312007688371No Hit
GCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGG171950.12065704797147399No Hit
ACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTG166560.11687489334183604No Hit
CCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA165590.11619424584819063No Hit
CGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAAC164980.11576620979548578No Hit
TTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGC162420.11396986177101953No Hit
GTTACGGGTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAG161350.11321904443266839No Hit
CCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATC161330.11320501046372725No Hit
CAAGGTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTC160200.1124120912185527No Hit
TTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGATCCTCCGGCAC154130.10815278164491587No Hit
GTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAG154100.10813173069150418No Hit
GGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTCCGAGT151440.1062652128223322No Hit
GGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGC150310.10547229357715765No Hit
CGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTC148760.10438466098421909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)