FastQCFastQC Report
Fri 21 Jul 2023
Syr1_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr1_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14251136
Sequences flagged as poor quality0
Sequence length20-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT6494544.557208632350432No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA4742423.327748749292688No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA2172441.5243977743247978No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA2073631.4550629507710824No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC1881071.319943897805761No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT1783481.2514651463574553No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC1370400.9616075518470948No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG1189930.8349720331066941No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA1056690.7414777320208017No Hit
GTCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCC1029770.7225880098260238No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG919530.6452327730224454No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG901460.632553082084123No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC847840.5949280113529195No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC839110.5888021839101107No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA723460.5076507585079534No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG723150.5074332319893656No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC689310.4836877565409523No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA636940.4469398088685702No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA614260.43102528808931445No Hit
GTCGTACTCCATTTTTGACGCCCTAGCGAAAGCTAGGTGGGTGTCTTGTC586320.4114198334785381No Hit
TAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCA574570.4031748767256168No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG569880.39988391100891885No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC559350.39249502636140726No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA542690.3808047302334354No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC490780.34437956384669965No Hit
GGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCT475790.3338611041253132No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC474930.33325764346084413No Hit
CTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGT474230.3327664545479041No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT473560.33229631658837583No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT440750.3092735905404313No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA426990.2996182199089252No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC421480.29575186146564036No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA417190.2927415751277653No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT415750.291731129364003No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC392880.27568328587980634No Hit
CTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTT375410.2634246140097182No Hit
GCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA371330.26056168434572513No Hit
CCGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAG361470.2536429376577418No Hit
GGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTC360040.2526395088784501No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG339720.2383809964342492No Hit
GTTGAATTCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGGAAAGT333480.23400239812461265No Hit
GGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCC326930.22940627329638844No Hit
AAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTGTG319790.22439614638440053No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG317510.22279627392511026No Hit
CCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTC313810.22019998967099885No Hit
TAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAG309600.21724583920888835No Hit
CAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCG295040.2070291098197365No Hit
GTTCGTTGCCAGATGTTAGGCCTCATTATATCAGTGGAAAACAGAAGTCA292430.20519767687291737No Hit
GGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGA290620.20392760268374394No Hit
CGTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAG290590.20390655173033223No Hit
GCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTC286520.20105063905080972No Hit
CGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGA278960.19574579879105777No Hit
GTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTC274530.1926372746705947No Hit
CGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCG269980.18944454673648473No Hit
GTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTG263740.18506594842684823No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT256650.18009090643721315No Hit
ACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA251450.17644207451251606No Hit
GCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGG248690.17450538679863836No Hit
GTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAAC245330.17214768001652642No Hit
TGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCT240230.168569017936535No Hit
GCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGC229790.16124328614925856No Hit
GACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACT228140.1600854837116143No Hit
AGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACC227850.15988199116196772No Hit
GCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAA227670.15975568544149743No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG224330.15741201262832663No Hit
CGCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA222150.15588231001374206No Hit
TCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGGAAAGTGGCGTTG214630.1506055376918724No Hit
GCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGC212400.14904075015493504No Hit
TATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTA211010.1480653893135256No Hit
CTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTCAACC207380.1455182239507082No Hit
TGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTA203810.14301316049471424No Hit
TCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCT203340.14268336222459738No Hit
GGAGGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTT198950.13960290604201658No Hit
GTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAGACTTCGC198680.13941344746131115No Hit
TAGACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGG195220.13698557083449348No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA192690.13521027376343894No Hit
GAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAAC192530.1350980020119098No Hit
TCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTA189440.13292975381050323No Hit
ACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTT189290.13282449904344468No Hit
ATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTCTGGC188960.1325929385559158No Hit
AGACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGT186170.1306351998886264No Hit
GAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAAT181950.1276740324420453No Hit
GAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTC180630.1267477904919299No Hit
CAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCT179250.12577944663499105No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA178750.1254285974114625No Hit
CTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGATCCTCCGGCA178200.12504266326558108No Hit
TTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGT177920.12484618770040508No Hit
CTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTCAACCCTGCT174290.12229902233758767No Hit
GACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTT171390.12026409684112199No Hit
CCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA170730.11980097586606428No Hit
GTTACGGGTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAG168750.11841161294089118No Hit
GCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGG168400.11816601848442117No Hit
ACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTG167740.11770289750936347No Hit
GGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTCCGAGT167140.1172818784411292No Hit
CCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATC165080.11583637964019149No Hit
CGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAAC164510.11543641152536892No Hit
TTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGC159090.11163320594231928No Hit
CAAGGTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTC157670.11063679414749814No Hit
GTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAG156610.10989299379361758No Hit
TTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGATCCTCCGGCAC151350.10620205996209706No Hit
CGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTC148010.10385838714892623No Hit
GGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGC144370.10130420480163828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)