FastQCFastQC Report
Fri 21 Jul 2023
Syr-MC3-big_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr-MC3-big_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11024848
Sequences flagged as poor quality0
Sequence length20-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT2537352.3014829773616836No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA703310.637931697561726No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG626860.5685883379072437No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA523860.475163013585312No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC368760.33448080191218965No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC345640.313509991248859No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA336700.30540103591450873No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA335850.3046300502283569No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA335420.30424002217536245No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT303910.275659129268721No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG263640.23913254858479682No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC251550.22816641100176618No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG246770.2238307503196416No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA242600.22004838524757891No Hit
GTTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACT241990.21949508963751702No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA236570.21457892208581925No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG233640.21192128907355456No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG209870.19036090112081364No Hit
TTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCG196970.17866005953097946No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC194090.17604777861790022No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA172450.1564193900904575No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT166940.151421588760226No Hit
CTCATGAATAACTGTGTTTTATACTCTACCGAGTGTAGTTTATCCTCTGC165340.14997032158629306No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG154370.1400200710250155No Hit
CTCATGAATAACTGTGTTTTATACTCTACCGAGTGCACTTTATCCTCTGC153940.13963004297202103No Hit
TGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAA152300.13814249411873977No Hit
CTGGAATGTTGTTTTTGGTGCTAAAGCTTGTGGTTCGCCAGCTTCACGAA150760.13674564946382933No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC150450.13646446644887983No Hit
CATAACTTGGCGTATTGGTGTGAACGACCAATTTGCCTTCAGGTACTTTC150080.13612886091490786No Hit
CGGCGATTAAAAACATTTTTGATATTTACGACCCTGAAATCATTGCCGTT147060.13338959412410947No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA142060.12885438420556908No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG141540.1283827223740409No Hit
CCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCAC141500.1283464406946926No Hit
CTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCC139000.1260788357354224No Hit
AAAACCTGTAAAACAGCCGAAACATCCGCGAGGGTGAATGCGGCTAAAAT136660.12395635749354549No Hit
ATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATC129340.11731681017280238No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA129090.11709004967687536No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT128900.11691771169997082No Hit
GAATAACTGTGTTTTATACTCTACCGAGTGTAGTTTATCCTCTGCCGAGC126850.11505827563336928No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC124390.11282695235344742No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT120810.10957974205177251No Hit
AAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTGTG116130.10533478556801872No Hit
GATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAAT115380.10465450408023766No Hit
GTCGTACTCCATTTTTGACGCCCTAGCGAAAGCTAGGTGGGTGTCTTGTC112840.10235061744161915No Hit
GTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAAAG111560.1011896037024728No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC111240.10089935026768623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)