FastQCFastQC Report
Fri 21 Jul 2023
Syr-MC1-big_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr-MC1-big_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18265905
Sequences flagged as poor quality0
Sequence length20-150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCG3830502.0970764930618No Hit
GTTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACT3104991.6998829239503874No Hit
TGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAA2741591.5009330224809556No Hit
CTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCC2351911.287595659782529No Hit
ATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATC2231141.2214779393629824No Hit
GATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAAT1995561.0925054083003278No Hit
CCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCAC1811560.9917712809740332No Hit
CTTTAATTGACTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGAT1796580.983570209086273No Hit
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT1470680.8051503607404068No Hit
ATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATA1129570.6184035228476225No Hit
CTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGGAT1064110.5825662621151265No Hit
GTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAA1035480.5668922508903884No Hit
TTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGA952830.5216440138060502No Hit
AAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAAT936470.5126874359633427No Hit
TCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCA928440.5082912672544831No Hit
GGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTC835900.45762857082635655No Hit
GTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAA826420.4524385733967192No Hit
CCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAAT747410.40918312013557495No Hit
CAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAG676630.3704333291999493No Hit
GTGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAA628990.34435194971177174No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG619380.33909078143130605No Hit
CGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCA569550.31181044684071224No Hit
GTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCT563680.3085968091917701No Hit
ATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGATTATTTCGTTCATC550650.30146330006643524No Hit
CCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGG544490.29809089667333755No Hit
CGTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATA542720.2971218781659053No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA527490.2887839392573212No Hit
ATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTA501450.2745278703683174No Hit
CTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCTTTAATT486360.2662665769913946No Hit
AAAAAATTGAAGCAGATTAAATCAGTCAATTAAAGGCCAAAGAAATTTGA473490.25922066275938693No Hit
TTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCT464680.2543974689455573No Hit
TTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTC457600.25052139491582814No Hit
ATTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAATCTG455030.2491144019417598No Hit
CAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAAAAAAATT437860.23971437495158332No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA431540.23625437666515836No Hit
ATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTG429410.23508826964773988No Hit
CTCAATCATTTTCAGGAACGAGTGAAAGAATCATAGTGTCAACCACTCAA423480.23184178391380006No Hit
CTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGA418450.22908801945482582No Hit
GAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAAT412390.2257703628700576No Hit
AAATAATTGATTGACAGGCCGGGAAAACAATCTGCAACAAGTCAGATCTT393310.2153246718407875No Hit
GAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATG379880.20797217548213462No Hit
CCGGCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGA373040.20422749379239627No Hit
CTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAAAAAAATTGAA366120.20043901465599434No Hit
GCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGATTT357840.19590597892631106No Hit
GTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAA351230.1922872148957306No Hit
AGAAATTTGAGTAAAATCAAATTATAATGAAATTAAGATAAAAGGGTAAC327500.17929579727913836No Hit
GAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAA321800.1761752292043564No Hit
AACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGG299910.16419115286102715No Hit
TTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAATCTGC292020.15987162968382895No Hit
CTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGATTATTTCGTTC284720.1558751126757749No Hit
CCGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCTGACTTGT284140.15555758118746374No Hit
TATAATTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAA282990.15492799289167442No Hit
ATCATTTTCAGGAACGAGTGAAAGAATCATAGTGTCAACCACTCAATAAC281270.15398634778840686No Hit
GGGTAACCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGA276600.15142967183941886No Hit
CGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCTGACTTGTT273900.14995150801452212No Hit
CCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACA273240.1495901790795474No Hit
AAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAATT273180.14955733099454968No Hit
CTCTTTTCTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTAC271500.14863758461461396No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA268880.14720321823638083No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA268350.14691306015223443No Hit
GAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTC266590.1459495163256351No Hit
TACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTT259820.14224315740172744No Hit
GGTACATTGATTTGTTTGCCAGCGTCGTAATTGTTATTGAGTGGTTGACA258270.14139458187262005No Hit
GGGATATCCGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCT258100.14130151229845989No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC255590.13992736740938924No Hit
TCCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAA255470.13986167123939383No Hit
AAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGT252210.13807692528785187No Hit
GCCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTG249290.13647831848463024No Hit
CTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAATG247970.13575566061468075No Hit
CCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAG247230.13535053423304239No Hit
CATAACTTGGCGTATTGGTGTGAACGACCAATTTGCCTTCAGGTACTTTC246210.1347921167880814No Hit
CGCCAACTTTTTGGAATCATCTTACTCTTTAGATCGGATTTTTTCTCATT237820.1301988595692357No Hit
AGATGAACGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAA237120.12981563191092912No Hit
CGGCGATTAAAAACATTTTTGATATTTACGACCCTGAAATCATTGCCGTT231700.1268483548994698No Hit
TGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAAT230240.126049051497859No Hit
GCTCTTTTCTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTA227610.12460921043879293No Hit
GAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAATTGGAAAC226200.12383728044134687No Hit
GAACGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAG223770.12250693299893983No Hit
GCCAACTTTTTGGAATCATCTTACTCTTTAGATCGGATTTTTTCTCATTA221960.12151601576817572No Hit
ATTATAATGAAATTAAGATAAAAGGGTAACCTCACAAAGAATTAAATGAA220540.12073861108989672No Hit
CTTTACTCGATACTTTTGATTTAAGAACTTTTTCTCGTTTCCAATTTTTA219660.12025683917659706No Hit
CGGCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGAT218430.11958345343414412No Hit
GTCGGTACATTGATTTGTTTGCCAGCGTCGTAATTGTTATTGAGTGGTTG211550.11581687302107395No Hit
TCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAATTAAAGG209080.11446462685533512No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT206140.11285507069044758No Hit
TAAAAGGGTAACCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATG204740.11208861537383447No Hit
GAAAAAGTTCTTAAATCAAAAGTATCGAGTAAAGAATCCAGTAAATAATG203910.11163421686469957No Hit
ATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAA202390.11080206537809104No Hit
AAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGT199970.10947719261651695No Hit
GAAATAATTGATTGACAGGCCGGGAAAACAATCTGCAACAAGTCAGATCT198590.10872168666156974No Hit
ATTAAATCAGTCAATTAAAGGCCAAAGAAATTTGAGTAAAATCAAATTAT195520.10704095964585383No Hit
CGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGA195390.10696978879502549No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC195290.10691504198669598No Hit
CAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAG195270.10690409262503006No Hit
CTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTACTTTTAAG188130.1029951705103032No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA187470.10263384157532845No Hit
TAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAA186820.10227798732118666No Hit
GAAAACCATGTCCAATTAAAAATTGGAAACGAGAAAAAGTTCTTAAATCA184000.10073412732629454No Hit
AATTGATTGACAGGCCGGGAAAACAATCTGCAACAAGTCAGATCTTCCAA183850.10065200711380028No Hit
GTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAA183380.10039469711465157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)