FastQCFastQC Report
Fri 21 Jul 2023
Syr-MC1-big_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr-MC1-big_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18265905
Sequences flagged as poor quality0
Sequence length20-150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCG3889382.129311413806214No Hit
GTTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACT3124801.710728266680463No Hit
TGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAA2730761.49500394313887No Hit
CTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCC2280961.2487527992727434No Hit
ATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATC2271861.2437708397147582No Hit
CTTTAATTGACTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGAT1959751.072900576237531No Hit
GATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAAT1952111.0687179200811567No Hit
CCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCAC1817620.9950889375588015No Hit
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT1556360.8520574261171291No Hit
CTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGGAT1094130.5990012539756449No Hit
GTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAA979650.5363271078000242No Hit
AAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAAT954390.5224980640159905No Hit
ATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATA945830.5178117372229846No Hit
TTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGA928320.5082255710844877No Hit
TCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCA926000.5069554451312431No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG816720.4471281329887569No Hit
GGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTC811020.444007564913975No Hit
GTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAA810910.44394734342481257No Hit
CCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAAT781290.4277313387976123No Hit
CAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAG705280.3861182897863533No Hit
GTGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAA649350.3554983998876595No Hit
ATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGATTATTTCGTTCATC621600.3403061605762211No Hit
CGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCA611660.334864327828268No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA553970.30328089410297493No Hit
GTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCT553560.30305643218882394No Hit
CCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGG532880.29173479222628174No Hit
CGTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATA525910.28791893968571497No Hit
ATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTA516090.2825428031077573No Hit
CTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCTTTAATT496160.2716317642076864No Hit
ATTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAATCTG482800.26431759061486415No Hit
TTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCT482480.2641424008282097No Hit
AAAAAATTGAAGCAGATTAAATCAGTCAATTAAAGGCCAAAGAAATTTGA479000.26223721189834287No Hit
TTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTC456820.250094369810858No Hit
CTCAATCATTTTCAGGAACGAGTGAAAGAATCATAGTGTCAACCACTCAA448920.24576937195282686No Hit
CTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGA441740.24183855111476818No Hit
CAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAAAAAAATT430940.23592589581518134No Hit
ATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTG429040.2348857064569207No Hit
GAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAAT426290.23338016922785926No Hit
CCGGCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGA422850.23149687902132413No Hit
AAATAATTGATTGACAGGCCGGGAAAACAATCTGCAACAAGTCAGATCTT394920.21620609545489258No Hit
GAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATG389680.2133373626984264No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA380300.20820211207711856No Hit
CTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAAAAAAATTGAA379400.20770939080215298No Hit
GCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGATTT377040.20641736612557657No Hit
GTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAA356070.19493696041887879No Hit
GAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAA335860.18387263045548524No Hit
AGAAATTTGAGTAAAATCAAATTATAATGAAATTAAGATAAAAGGGTAAC331750.18162253663314246No Hit
CTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGATTATTTCGTTC313160.17144510496468693No Hit
CCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACA308460.16887200497320007No Hit
TATAATTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAA304840.16689017051167188No Hit
TTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAATCTGC303960.16640839859837223No Hit
AACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGG299800.16413093137186469No Hit
ATCATTTTCAGGAACGAGTGAAAGAATCATAGTGTCAACCACTCAATAAC298070.16318381158776418No Hit
GGGTAACCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGA288330.15785147245647013No Hit
CTCTTTTCTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTAC287350.15731495373484095No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA285500.15630213778074506No Hit
CCGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCTGACTTGT281290.15399729715007276No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA278040.15221802587936378No Hit
AAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAATT276230.15122710864859967No Hit
GAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTC269640.14761929397968512No Hit
CGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCTGACTTGTT264830.14498597249903578No Hit
TCCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAA264560.1448381561165461No Hit
CCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAG260100.14239644846505006No Hit
TACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTT257380.14090733527848742No Hit
GGGATATCCGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCT257030.14071572144933414No Hit
CTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAATG256190.1402558482593663No Hit
AAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGT252310.13813167209618138No Hit
GCCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTG245640.1344800599806032No Hit
CGCCAACTTTTTGGAATCATCTTACTCTTTAGATCGGATTTTTTCTCATT242510.1327664848798896No Hit
GGTACATTGATTTGTTTGCCAGCGTCGTAATTGTTATTGAGTGGTTGACA242200.13259676977406815No Hit
AGATGAACGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAA242040.1325091748807409No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC241560.1322463902007593No Hit
CATAACTTGGCGTATTGGTGTGAACGACCAATTTGCCTTCAGGTACTTTC241450.13218616871159683No Hit
GCTCTTTTCTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTA237850.13021528361173454No Hit
ATTATAATGAAATTAAGATAAAAGGGTAACCTCACAAAGAATTAAATGAA233470.12781737340690208No Hit
GAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAATTGGAAAC232170.1271056648986185No Hit
TGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAAT228850.12528807086207883No Hit
GAACGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAG227720.1246694319279554No Hit
CTTTACTCGATACTTTTGATTTAAGAACTTTTTCTCGTTTCCAATTTTTA226270.12387560320717753No Hit
CGGCGATTAAAAACATTTTTGATATTTACGACCCTGAAATCATTGCCGTT224460.12288468597641343No Hit
TCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAATTAAAGG222470.12179522449065623No Hit
TAAAAGGGTAACCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATG216070.11829142875756772No Hit
CGGCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGAT215910.11820383386424049No Hit
ATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAA213280.11676399280517444No Hit
GCCAACTTTTTGGAATCATCTTACTCTTTAGATCGGATTTTTTCTCATTA211620.1158551957869046No Hit
GAAAAAGTTCTTAAATCAAAAGTATCGAGTAAAGAATCCAGTAAATAATG204210.11179845728968808No Hit
CAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAG202260.11073089452726269No Hit
ATTAAATCAGTCAATTAAAGGCCAAAGAAATTTGAGTAAAATCAAATTAT200910.10999181261481432No Hit
CGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGA200760.10990969240232006No Hit
AAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGT200630.10983852155149171No Hit
GAAATAATTGATTGACAGGCCGGGAAAACAATCTGCAACAAGTCAGATCT200310.10966333176483728No Hit
GTCGGTACATTGATTTGTTTGCCAGCGTCGTAATTGTTATTGAGTGGTTG199570.10925820538319891No Hit
CTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTACTTTTAAG194280.10636209922256795No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT192140.10519051752431648No Hit
GTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAA190990.10456092922852714No Hit
TAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAA190670.10438573944187271No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC189110.1035316892319324No Hit
GTCCAATTAAAAATTGGAAACGAGAAAAAGTTCTTAAATCAAAAGTATCG186090.10187833562038125No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA185010.10128707009042257No Hit
GAAAACCATGTCCAATTAAAAATTGGAAACGAGAAAAAGTTCTTAAATCA184560.10104070945293978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)