FastQCFastQC Report
Fri 21 Jul 2023
MC3-big_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC3-big_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12289014
Sequences flagged as poor quality0
Sequence length20-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT3467492.821617747363621No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG1098830.8941563578656515No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA1067330.8685237074349496No Hit
TTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCG824550.6709651400836552No Hit
GTTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACT707080.5753756973504953No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA617050.5021151412147468No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA584090.47529443777995534No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC578910.47107929082023997No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA577930.4702818305846181No Hit
TGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAA539240.43879842597624186No Hit
CTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCC505090.41100937796962395No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT451260.3672060264558247No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA445630.36262469877567066No Hit
ATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATC438900.3571482626677779No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC436590.35526853496952643No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG412220.33543781462044064No Hit
GATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAAT399810.32533936408567843No Hit
CTTTAATTGACTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGAT365460.29738756909219893No Hit
CCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCAC360650.2934735040581775No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA346890.2822765113620995No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC341900.2782159740399026No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG335950.2733742511807701No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG300090.24419371643648546No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG286800.2333791791595322No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC261040.2124173672517584No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA253680.20642827813525155No Hit
GTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAA250120.2035313817691151No Hit
CTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGGAT238070.19372587581070377No Hit
ATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATA236580.19251341075858486No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA220200.17918443253461996No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG218600.17788245663972715No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT213150.1734476012477486No Hit
TTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGA207730.16903715790379928No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT201460.16393503986568816No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA197390.16062313868305464No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC196510.1599070519408636No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT192710.15681485919049323No Hit
TCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCA191890.1561475965443607No Hit
GGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTC190160.15473983510800787No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG188900.1537145290907798No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA188020.15299844234858875No Hit
AAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAAT186150.1514767580214328No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC185860.1512407748904835No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC184130.14983301345413066No Hit
CCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGGC183620.14941800863763358No Hit
GCCGAGTTCTGTTTAAATAGTCATCTATCTCGATTTATTGTTACCAATAA178580.1453167845687213No Hit
GTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAA175170.14254194844273105No Hit
GTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAAAG173700.14134575808929828No Hit
CCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAAT169330.13778973642637238No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG166110.13516950993790064No Hit
CTCATGAATAACTGTGTTTTATACTCTACCGAGTGCACTTTATCCTCTGC165880.1349823509030098No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC161870.13171927381643472No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA155810.12678804011452832No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC155760.1267473533678129No Hit
GCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAA153480.12489203771759069No Hit
CCGAAACCCAAAACCACAGGTTGAAATGGGAGGCGTGCAGCATTAATTAT149940.12201141605014039No Hit
CAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAG149310.12149876304152636No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT146800.11945628835641331No Hit
AAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTGTG146730.11939932691101174No Hit
CTCATGAATAACTGTGTTTTATACTCTACCGAGTGTAGTTTATCCTCTGC144670.11772303294633726No Hit
GAATAACTGTGTTTTATACTCTACCGAGTGTAGTTTATCCTCTGCCGAGC144000.11717783054035091No Hit
GAATAACTGTGTTTTATACTCTACCGAGTGCACTTTATCCTCTGCCGAGC137950.11225473418778756No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA136940.1114328619041365No Hit
TCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTA136440.1110259944369825No Hit
GTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCT132770.10803958722807216No Hit
GCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAA128940.10492298242967256No Hit
CTCAATCCTTTTCCAGATTGACTGACACGGTCATTGAAAAGTAAATAAAC128780.10479278484018327No Hit
CAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAA128010.10416620894076613No Hit
TGAAGCTTGAGTAGCGAGTCGATGTTGCTGATCATCGTAAAAAGTGGCGC127690.10390581376178756No Hit
GTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGATCA126390.10284795834718716No Hit
GGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGATCATCGTAAAAA126240.10272589810704097No Hit
AAAACCTGTAAAACAGCCGAAACATCCGCGAGGGTGAATGCGGCTAAAAT125850.10240854148266085No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC125290.10195284991944838No Hit
TGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAA123300.10033351740017547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)