FastQCFastQC Report
Fri 21 Jul 2023
MC3-big_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC3-big_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12289014
Sequences flagged as poor quality0
Sequence length20-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT3539392.8801252891403655No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG1143600.9305872708746202No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA1073040.8731701339098483No Hit
TTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCG860330.7000805760331952No Hit
GTTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACT702680.5717952636395401No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA641020.5216203675901093No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA601020.48907097021778967No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA566750.46118427401905476No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC562630.4578316860897058No Hit
TGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAA556610.4529330017851717No Hit
ATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATC472080.3841479877881171No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA450690.36674219754326914No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC437350.3558869735196005No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT424510.3454386169630859No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG419930.3417117109639553No Hit
CTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCC413260.33628409895212097No Hit
GATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAAT407960.3319713038002886No Hit
CTTTAATTGACTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGAT381230.310220169006236No Hit
CCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCAC365050.29705393776913264No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG355490.28927463179714824No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA345660.2812756173929007No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC328170.2670433933918539No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG309760.2520625332512438No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG282720.23005914062755564No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC281880.22937560328273693No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA251960.2050286540482418No Hit
CTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGGAT236330.19230997702500788No Hit
GTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAA233220.18977926137931No Hit
CCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGGC230080.1872241336855829No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA218870.1781021650719903No Hit
ATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATA216190.17592135544804488No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT212620.17301632173256537No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG211670.17224327354497276No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC211100.17177944463241723No Hit
TTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGA208910.16999736512628272No Hit
AAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAAT196990.16029764470933144No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT195410.1590119435131248No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA194550.15831213146961995No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT193900.15778320376231975No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC193660.1575879073780858No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC193280.1572786881030488No Hit
TCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCA192440.15659515075823008No Hit
GTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAAAG188060.15303099174596108No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG186770.15198127368070377No Hit
GGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTC182610.1485961363539825No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA181590.14776612672098835No Hit
CCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAAT172560.14041810026418716No Hit
CTCATGAATAACTGTGTTTTATACTCTACCGAGTGCACTTTATCCTCTGC172560.14041810026418716No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG169190.13767581353556924No Hit
GTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAA168160.136837666553232No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC163810.13329791958899226No Hit
GCCGAGTTCTGTTTAAATAGTCATCTATCTCGATTTATTGTTACCAATAA161110.13110083526636065No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC157660.1282934497429981No Hit
GCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAA156560.12739834131525932No Hit
CCGAAACCCAAAACCACAGGTTGAAATGGGAGGCGTGCAGCATTAATTAT154420.1256569485558402No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA153270.12472115338138601No Hit
CTCATGAATAACTGTGTTTTATACTCTACCGAGTGTAGTTTATCCTCTGC152580.1241596762767135No Hit
CTCAATCCTTTTCCAGATTGACTGACACGGTCATTGAAAAGTAAATAAAC149280.12147435099349711No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT147900.12035139678415209No Hit
GAATAACTGTGTTTTATACTCTACCGAGTGTAGTTTATCCTCTGCCGAGC147610.12011541365320277No Hit
AAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTGTG146980.11960276064458873No Hit
CAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAG146730.11939932691101174No Hit
GAATAACTGTGTTTTATACTCTACCGAGTGCACTTTATCCTCTGCCGAGC139700.11367877032282656No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA138210.11246630527070765No Hit
TCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTA137900.11221404744107216No Hit
CAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAA135520.11027735829741915No Hit
GCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAA133420.10856851493537235No Hit
GTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCT129040.10500435592310335No Hit
TGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAA128850.10484974628558483No Hit
AAAACCTGTAAAACAGCCGAAACATCCGCGAGGGTGAATGCGGCTAAAAT126340.10280727160047176No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC124080.10096823064893572No Hit
GTGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAA123700.10065901137389867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)