FastQCFastQC Report
Fri 21 Jul 2023
Syr4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21385198
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT6837203.197164693074153No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA4526172.1164966534329026No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA2136850.9992191795465255No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC1829050.8552878491001112No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA1807490.8452061093846313No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT1690810.790645005952248No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC1324140.6191852888151889No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG1173860.5489123832288109No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA980840.4586536912120244No Hit
GTCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCC943050.4409825899203739No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA889230.4158156496844219No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG861940.40305448656589477No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC847770.3964284080979751No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG833890.3899379374462654No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC734460.34344316101258454No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG731900.3422460713246611No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA679160.3175841533008018No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC649730.30382229802127625No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG645720.30194716925230247No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC641090.2997821203245348No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA641010.29974471127178715No Hit
TAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCA639370.2989778256904612No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG636780.2977667076077575No Hit
GGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTC599260.28022186186913023No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA550320.2573368738507822No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA529930.2478022415317361No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC499870.23374578996182313No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT491660.22990668592360008No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA463140.21657035861907847No Hit
GGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCT452040.21137985255034816No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT434910.203369639130767No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC426410.19939492727633384No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT422830.19772087216587847No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA418500.1956961071859143No Hit
CTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGT418110.19551373805376973No Hit
GTCGTACTCCATTTTTGACGCCCTAGCGAAAGCTAGGTGGGTGTCTTGTC406050.18987432335206808No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC399800.1869517411061614No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG380470.17791277873602107No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC370660.17332549364284586No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG351730.16447357653644357No Hit
CTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTT343630.16068590994574847No Hit
CCGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAG337970.15803921946385532No Hit
GCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA331430.15498102940173852No Hit
CCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTC321400.15029086941350742No Hit
GGAGGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTT315480.14752259951018457No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT311910.14585322053132266No Hit
GGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCC306830.14347774568184965No Hit
CAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCG305790.1429914279961308No Hit
GGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGA297220.13898398322054348No Hit
AAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTGTG295950.13839011450817523No Hit
GGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTCCGAGT289530.13538803802517985No Hit
GTTGAATTCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGGAAAGT287440.13441072652214864No Hit
TAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAG282850.13226438212075473No Hit
GCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAA277360.12969718587595028No Hit
CGTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAG271900.12714401802592615No Hit
CATAACTTGGCGTATTGGTGTGAACGACCAATTTGCCTTCAGGTACTTTC264390.12363224319924464No Hit
CGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGA262510.12275313045967588No Hit
CGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCG260390.12176179056186433No Hit
GCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTC257480.12040103626817017No Hit
GCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTCC255000.11924135563299437No Hit
GTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAGACTTCGC253250.11842303260414049No Hit
CAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAA245110.11461666148707156No Hit
GTGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGA243590.11390588948486705No Hit
GGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGATCATCGTAAAAA240550.11248434548045802No Hit
GTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAAAG240330.11238147058540211No Hit
GTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTC237320.11097395497577343No Hit
GCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAA234500.10965528586642032No Hit
GTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTG231280.10814957149332917No Hit
TGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCT230880.10796252622959114No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA223240.10438996169219476No Hit
AGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACC222540.10406263248065321No Hit
TGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAA219320.10255691810756207No Hit
GCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGC216170.10108393665562508No Hit
TATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTA214510.10030769881111225No Hit
GCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGC214380.10024690910039738No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)