FastQCFastQC Report
Fri 21 Jul 2023
Syr2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15701804
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT5754593.664922833070646No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA2788281.7757704783475836No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA1613841.027805467448199No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC1459790.9296957215871501No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT1343400.8555704809460111No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA1307500.8327068660390871No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA925530.5894418246463909No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC893770.5692148494529673No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG774630.49333821769778813No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG724580.4614628994222575No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC710000.45217734217036465No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG704930.44894841382557066No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG637010.4056922376562591No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA618460.39387830850518835No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA604520.3850003477307448No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC602540.3837393461286359No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA591270.3765618269085514No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG567010.3611113729352372No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC565790.36033439215009944No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA529190.33702496859596515No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC527380.33587223480817874No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG498870.31771508547680255No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA464670.2959341487131033No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT461610.29398532805529864No Hit
GTCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCC378750.24121432161552903No Hit
TAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCA367260.23389669110632128No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG346190.22047785082529373No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC343840.2189812075096594No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA341010.2171788668359381No Hit
GGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCT336590.21436390366355354No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT334130.21279720470335764No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC329200.2096574380880057No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC315730.20107880597668906No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG312030.19872238884143506No Hit
GGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTC294690.18767907178054188No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA289930.18464757297951243No Hit
CTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGT269340.17153443005657185No Hit
TAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAG256600.16342071267734587No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT256430.16331244486302338No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC252040.1605165877755193No Hit
GGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCC244810.15591202131933377No Hit
GCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAA244770.1558865465394932No Hit
CTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTT230870.14703406054489024No Hit
TCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTA229990.1464736153883974No Hit
CCGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAG226100.14399619304890063No Hit
GCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA220930.14070357775450515No Hit
ACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA211450.13466605493228676No Hit
AGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACC210510.13406739760603303No Hit
GAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAAC201790.12851389560078574No Hit
GTGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGA201470.12831009736206106No Hit
CATAACTTGGCGTATTGGTGTGAACGACCAATTTGCCTTCAGGTACTTTC198650.12651412538329992No Hit
CAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAA197900.12603647326128894No Hit
TGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTA196540.1251703307467091No Hit
GTCGTACTCCATTTTTGACGCCCTAGCGAAAGCTAGGTGGGTGTCTTGTC194440.12383290480507846No Hit
GTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAAC182320.11611404651338152No Hit
TGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCT180280.11481483274151175No Hit
GTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAGACTTCGC178210.11349651288476152No Hit
GTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAAAG177560.11308254771235204No Hit
CGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGA176150.11218456172297143No Hit
GTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTC175370.11168780351608007No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT174660.11123562617390971No Hit
TATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTA173860.11072613057709801No Hit
CGGCGATTAAAAACATTTTTGATATTTACGACCCTGAAATCATTGCCGTT172420.109809038502837No Hit
TGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAA171830.1094332855001884No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA169320.10783474306519175No Hit
GGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGA168140.10708323705989453No Hit
TTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGT164950.10505162336760795No Hit
GGAGGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTT159970.10188001327745524No Hit
CGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCG158680.10105845162759641No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)