FastQCFastQC Report
Fri 21 Jul 2023
Syr2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15701804
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT5778973.6804497113834813No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA2746131.7489264290905682No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA1623681.0340722632889825No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC1432720.912455664330035No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA1323470.84287767189044No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT1259140.8019078572118209No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA952230.6064462401899807No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC873620.5563819291082732No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG791370.5039994130610724No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC757610.48249869887561964No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG755720.481295015528152No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG693460.4416435207062832No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG660100.4203975543192362No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA628430.40022789738045383No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA604200.3847965494920202No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC593580.3780329954443451No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC582630.37105927446298526No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA579200.3688748120916552No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG572290.36447404387419435No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC542640.3455908633173615No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA524070.33376419677637037No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG513420.32698153664381496No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA475820.30303524359366607No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT451060.28726635487234464No Hit
GTCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCC385730.24565967069771089No Hit
TAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCA375850.2393674000770867No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT352220.22431817388626174No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG348440.22191080719132653No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA348380.22187259502156564No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC346910.22093639686242422No Hit
GGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCT336280.21416647411978906No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC333900.2126507247192743No Hit
GGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTC328420.20916067988111428No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG318770.20301488924457345No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC314100.20004070869818524No Hit
CTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGT291900.18590220588666118No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA289430.1843291382315051No Hit
TAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAG263790.16799980435369083No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT256280.16321691443862119No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC251810.16037010779143593No Hit
GCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAA246720.15712844205672163No Hit
GGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCC239040.15223728432732953No Hit
CTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTT235090.14972164981807187No Hit
TCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTA235040.14968980634327114No Hit
GCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA222040.14141050289508134No Hit
CCGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAG215120.13700336598266033No Hit
ACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA210810.13425845845483741No Hit
AGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACC210620.13413745325059465No Hit
CAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAA202730.12911255292703946No Hit
GTGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGA197770.12595368022680706No Hit
CATAACTTGGCGTATTGGTGTGAACGACCAATTTGCCTTCAGGTACTTTC197340.1256798263435208No Hit
GTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAGACTTCGC194870.12410675868836474No Hit
GTCGTACTCCATTTTTGACGCCCTAGCGAAAGCTAGGTGGGTGTCTTGTC193120.12299223707033918No Hit
GAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAAC192820.12280117622153479No Hit
TGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTA191630.12204330152127743No Hit
GTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAAAG190070.12104978510749465No Hit
GTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAAC185680.11825392801999056No Hit
TGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAA182950.11651527429587072No Hit
TGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCT178530.11370031112348619No Hit
TATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTA177890.11329271464603685No Hit
GTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTC177540.11306981032243174No Hit
GGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGA175710.11190433914472502No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT174050.1108471357813408No Hit
GGAGGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTT171140.10899384554793831No Hit
TTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGT170440.10854803690072809No Hit
CGGCGATTAAAAACATTTTTGATATTTACGACCCTGAAATCATTGCCGTT164770.10493698685832531No Hit
CCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGGC164160.10454849646575642No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA164060.10448480951615495No Hit
CGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGA162070.10321743921908591No Hit
GCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGC157700.10043431952150213No Hit
GTTACGGGTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAG157500.10030694562229919No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)