FastQCFastQC Report
Fri 21 Jul 2023
Syr1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14336208
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT6536684.559559961741626No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA4954153.45569065404185No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA2246251.566836920892889No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA2047901.4284809483790972No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC1971791.3753915958808633No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT1889991.3183332719502954No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC1389590.9692869969520531No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG1221160.8518012573478286No Hit
GTCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCC1072120.7478407121325249No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA1052750.7343294684340518No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG908590.6337728916879554No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC871610.6079780650503954No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC864880.6032836577147876No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG847010.5908187157998823No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG744580.5193702546726442No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA734690.51247163824632No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC676230.4716937700680682No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA654720.45668980249170493No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA631790.4406953358935641No Hit
GTCGTACTCCATTTTTGACGCCCTAGCGAAAGCTAGGTGGGTGTCTTGTC605440.4223153012288884No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC604010.42131782686188707No Hit
TAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCA598680.41759996785760917No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG585380.40832275870997403No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA572040.3990176481814438No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC520980.36340153546879345No Hit
GGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCT502950.3508249880303076No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC481600.3359326259775249No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT479670.3345863843493342No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT466370.3253091752016991No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA436860.30472493144630713No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC419500.292615732137815No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA419190.2923994964358776No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT413040.2881096591232493No Hit
CTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGT406540.28357568472778855No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC396650.2766770683014644No Hit
CTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTT390940.27269414617868265No Hit
GCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA375150.26168007607032484No Hit
CCGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAG361830.2523889162322422No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG347910.2426792356807323No Hit
GGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCC340920.23780346936930602No Hit
GTTGAATTCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGGAAAGT339660.23692457587110904No Hit
CCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTC336680.23484592299442084No Hit
AAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTGTG326790.22794730656809664No Hit
GGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTC326400.227675268104369No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG323200.22544315763275755No Hit
TAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAG314210.21917232227657413No Hit
CAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCG310940.21689138438839617No Hit
GGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGA301660.21041826402072292No Hit
CGTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAG297830.20774670680001295No Hit
GCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTC297440.2074746683362853No Hit
CGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCG291700.20347082017783225No Hit
GTTCGTTGCCAGATGTTAGGCCTCATTATATCAGTGGAAAACAGAAGTCA289440.20189439215725666No Hit
CGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGA283860.19800214952238418No Hit
GTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTC279500.19496089900481356No Hit
GTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTG270230.1884947539823641No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT264370.1844072016812256No Hit
ACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA249260.17386745504808523No Hit
GTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAAC249190.17381862763151873No Hit
TGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCT246200.1717329994096068No Hit
AGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACC241980.16878940372516915No Hit
GACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACT237900.16594346287386455No Hit
GCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAA232750.16235116008361486No Hit
TGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTA227100.15841009003217588No Hit
TCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGGAAAGTGGCGTTG225910.15758002395054538No Hit
CGCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA222780.1553967408955004No Hit
GCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGC219250.152934444031504No Hit
GCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGC218260.1522438848543492No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG214520.14963510574065333No Hit
GCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGG212740.14839349429081944No Hit
TCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCT212270.1480656530653015No Hit
CTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTCAACC211260.14736114319769913No Hit
GAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAAC210180.1466078059135303No Hit
TAGACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGG209750.1463078660689075No Hit
TATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTA206540.14406878025207223No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA204690.14277834138567186No Hit
AGACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGT197320.13763751195574173No Hit
ACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTT193930.13527286992487833No Hit
ATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTCTGGC193360.13487527524712253No Hit
GTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAGACTTCGC190560.1329221785844625No Hit
GAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTC189220.1319874823244752No Hit
TCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTA188180.13126204642120148No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA186000.1297414211624162No Hit
GGAGGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTT185400.129322900448989No Hit
GAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAAT184310.12856258781959637No Hit
CTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGATCCTCCGGCA181180.12637930476455142No Hit
CTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTCAACCCTGCT180970.1262328225148519No Hit
CAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCT180600.12597473474157184No Hit
CAAGGTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTC179380.12512374262426995No Hit
GCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGG178790.12471219725606661No Hit
GACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTT178150.12426577516174431No Hit
TTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGT175930.12271724852206385No Hit
ACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTG171120.11936210746942288No Hit
TTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGC170990.11927142798151366No Hit
CGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAAC168530.11755549305646235No Hit
CCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA167010.1164952405824469No Hit
CCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATC164010.11440263701531116No Hit
GTTACGGGTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAG161500.11265182536414092No Hit
TTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGATCCTCCGGCAC161160.1124146636265322No Hit
GGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTCCGAGT155150.10822248114703693No Hit
GTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAG154970.10809692493300878No Hit
GGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGC150530.1049998716536479No Hit
CGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTC149570.10433023851216446No Hit
CTCAAACCTTGGCGCCTCGATGGGGAATTCAACCCTGCTACAGCGGATTG147640.10298399688397379No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)