FastQCFastQC Report
Fri 21 Jul 2023
Syr1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14336208
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT6624634.620908122984823No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA4936483.4433652190314206No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA2267161.5814223677558252No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA2094151.4607419200391067No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC1917981.3378572632316719No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT1807111.2605216107355586No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC1417390.9886784566741778No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG1250800.8724761805911299No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA1059950.7393517169951775No Hit
GTCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCC1043750.7280516577326446No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG920780.6422758375157502No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG913860.6374488986208906No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC874300.6098544329155938No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC845510.5897724140163145No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA729100.5085724202662238No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG728310.5080213679935447No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC690730.4818080206425576No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA640990.447112653499447No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA618000.43107633482996344No Hit
GTCGTACTCCATTTTTGACGCCCTAGCGAAAGCTAGGTGGGTGTCTTGTC603840.4211992459930827No Hit
TAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCA594760.4148656325298852No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG582750.40648824291611835No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC570900.39822245882593216No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA563140.3928095909322744No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC505760.35278506003819143No Hit
GGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCT482140.3363092946196093No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT481140.3356117600972307No Hit
CTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGT480520.335179288693356No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC478460.3337423675772561No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT447780.3123420084306812No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA434740.30324615825886453No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCACATCTCGTAT434320.30295319375946556TruSeq Adapter, Index 19 (97% over 37bp)
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA424170.29587321835732294No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC422050.29439444516988034No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT417220.2910253534267918No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC393490.2744728592107481No Hit
CTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTT393050.27416594402090144No Hit
GCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA374820.2614498896779399No Hit
CCGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAG365670.25506744879817594No Hit
GGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTC362320.25273070814820764No Hit
GTTGAATTCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGGAAAGT344190.24008440725748398No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG342020.2385707573439225No Hit
AAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTGTG334270.23316486479548845No Hit
GGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCC332470.23190930265520698No Hit
CCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTC319260.22269487161458595No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG318620.22224844952026365No Hit
TAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAG310150.21634033211571707No Hit
GGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGA305880.2133618597051605No Hit
CAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCG299960.2092324553326793No Hit
CGTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAG298540.20824195631090175No Hit
GTTCGTTGCCAGATGTTAGGCCTCATTATATCAGTGGAAAACAGAAGTCA292930.2043287876403579No Hit
GCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTC290890.2029058172147056No Hit
CGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCG282230.19686516825090705No Hit
CGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGA280670.19577701439599648No Hit
GTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTC278010.19392157256646947No Hit
GTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTG269500.18798555378102774No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT266350.1857883200355352No Hit
ACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA253560.17686685349431314No Hit
GCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGG250860.17498351028389098No Hit
GTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAAC247680.1727653505027271No Hit
TGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCT245210.171042440232452No Hit
GCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGC241330.1683360062856231No Hit
AGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACC232650.162281406631377No Hit
GCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAA231680.16160479814466977No Hit
GACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACT230630.16087238689617228No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG225490.15728705945114635No Hit
CGCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA223810.15611520145355035No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCACATCGCGTAT222850.15544556831206688TruSeq Adapter, Index 19 (97% over 37bp)
TCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGGAAAGTGGCGTTG220140.15355524975642093No Hit
GCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGC215970.15064653079810225No Hit
TATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTA211890.14780058994679765No Hit
CTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTCAACC210350.14672638678233463No Hit
TAGACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGG208290.14528946566623474No Hit
TCCAACTCTCAACGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCT206700.14418038577565281No Hit
TGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTA206340.14392927334759653No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA200160.1396185099992969No Hit
GTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAGACTTCGC199550.13919301394064595No Hit
GGAGGCGAGGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTT199420.13910233445273673No Hit
GAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAAC195970.13669584035053065No Hit
ATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCTCTGGC192660.1343870010814575No Hit
AGACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGT192030.13394755433235903No Hit
ACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTT191180.13335464998833724No Hit
TCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTA189670.13230137285954557No Hit
GAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTC188290.1313387752186631No Hit
GAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAAT188270.13132482452821553No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA187720.13094118054090734No Hit
CTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGATCCTCCGGCA182520.12731400102453871No Hit
CAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCT182340.12718844481051056No Hit
CTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATTCAACCCTGCT182130.12704196256081104No Hit
TTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGT178240.12432855326875837No Hit
CAAGGTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTC176780.12331015286608565No Hit
GACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTT176710.12326132544951915No Hit
GCAGGGTTGAATTCCCCATCGAGGCGCCAAGGTTTGAGGTCTGCCAGAGG175220.12222199901117507No Hit
ACGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTG172640.1204223599434383No Hit
CCCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAA172170.12009451871792039No Hit
GGGGAGTCGAACCCCTGTCCGAAAATCCATTCACAGGAGCTTCTCCGAGT170460.11890173468465301No Hit
GTTACGGGTTAGCTTTAGCAGCGTAATACGCTCTAAAGTTTAATACTAAG169060.117925186353323No Hit
CGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAAC168410.11747178891377694No Hit
CCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATC167870.11709512027169247No Hit
TTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAGGCGC167710.1169835147481119No Hit
TTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGATCCTCCGGCAC157740.11002909555999744No Hit
GTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAG157670.10998026814343095No Hit
CGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTC148900.1038628903821708No Hit
CTCAAACCTTGGCGCCTCGATGGGGAATTCAACCCTGCTACAGCGGATTG148880.10384893969172322No Hit
GGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGC144730.1009541714238521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)