FastQCFastQC Report
Fri 21 Jul 2023
Syr-MC2-big_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr-MC2-big_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9616518
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT2085602.168768362935524No Hit
TTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCG1003641.0436625814042046No Hit
GTTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACT999921.0397942373736524No Hit
TGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAA847740.8815456904463757No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA782800.814016050300119No Hit
CTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCC661020.6873797771709054No Hit
ATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATC633240.6584919822330703No Hit
GATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAAT601450.625434278810688No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG564400.586906820119299No Hit
CCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCAC544340.5660468789222877No Hit
CTTTAATTGACTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGAT416350.43295296696787755No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA406080.4222734257867557No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA380910.39609971093487273No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA356200.3704043396996709No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC353740.367846241227854No Hit
GTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAA330620.3438042751024851No Hit
ATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATA326280.3392912070668406No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC293720.30543279802523116No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT284320.2956579502060933No Hit
CTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGGAT282680.293952551224882No Hit
TCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCA282140.29339101741399537No Hit
GGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTC276660.28769248911092354No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA272020.2828674578470087No Hit
GTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAA248550.25846153462199106No Hit
TTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGA247890.25777521551979626No Hit
AAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAAT243400.2531061658700166No Hit
CCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAAT240460.2500489262329671No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG226690.2357298140553577No Hit
GTGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAA215610.22420797215790578No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC212010.22046441341866152No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG211300.21972610044508836No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA200640.208641007067215No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG195650.20345201870365137No Hit
CAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAG193240.2009459141032128No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG191600.19924051512200153No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA171060.17788143275975773No Hit
GTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCT170630.17743428546590356No Hit
CCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGG170090.17687275165501692No Hit
CGTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATA167970.17466821150857306No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC162640.16912566481963637No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG154030.16017232016827712No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA153570.15969397655159592No Hit
CGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCA152360.1584357248642388No Hit
ATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGATTATTTCGTTCATC150230.15622078594351926No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT142750.14844250278531168No Hit
CTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCTTTAATT139910.14548925089101897No Hit
ATTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAATCTG138610.14413741023518076No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA136140.14156891298908814No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT135380.14077860614413656No Hit
TTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTC134730.14010268581621746No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC131460.13670228662807057No Hit
CTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGA129730.1349032986783782No Hit
ATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTA129580.134747317064243No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC129200.13435216364176722No Hit
GAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAAT124610.12957912624923076No Hit
TTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCT124080.1290279912126198No Hit
AAAAAATTGAAGCAGATTAAATCAGTCAATTAAAGGCCAAAGAAATTTGA123900.12884081327565758No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG122830.12772814442815997No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA121360.1261995246096352No Hit
ATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTG119560.12432774524001307No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC118720.12345424820085606No Hit
CCGGCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGA114480.11904516790796835No Hit
GAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATG111070.11549918587996195No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG110710.11512483000603753No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG109830.11420973786977781No Hit
CAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAAAAAAATT109240.11359621018751279No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT106380.1106221607446687No Hit
GCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGATTT103870.10801206840147339No Hit
CTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAAAAAAATTGAA101560.10560995154379163No Hit
AAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTGTG100200.10419571824229934No Hit
CATAACTTGGCGTATTGGTGTGAACGACCAATTTGCCTTCAGGTACTTTC99250.10320783468610988No Hit
TATAATTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAA99130.10308304939480174No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC98190.10210556461288794No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA96820.10068093253711999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)