FastQCFastQC Report
Fri 21 Jul 2023
Syr-MC1-big_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr-MC1-big_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18378784
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCG3841242.090040342168448No Hit
GTTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACT3106471.6902478422946807No Hit
TGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAA2744871.4934992434755205No Hit
CTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCC2362161.2852645746312705No Hit
ATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATC2236021.216631089412662No Hit
GATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAAT1997901.0870686548141595No Hit
CTTTAATTGACTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGAT1853671.0085922985982096No Hit
CCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCAC1813700.9868443962342667No Hit
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT1521470.8278404055458729No Hit
ATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATA1131500.6156555297673666No Hit
CTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGGAT1066370.580217929543108No Hit
GTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAA1038400.5649992948390927No Hit
TTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGA956940.5206764495409489No Hit
AAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAAT948440.5160515516151667No Hit
TCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCA929180.5055720770209824No Hit
GGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTC840020.45705961830771835No Hit
GTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAA829780.4514879765712465No Hit
CCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAAT748320.4071651312731028No Hit
CAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAG678570.36921376299977193No Hit
GTGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAA629180.3423403855227854No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG622630.33877649359174145No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA590520.3213052615450511No Hit
ATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGATTATTTCGTTCATC587470.31964573934815277No Hit
CGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCA576980.3139380712020991No Hit
GTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCT567230.30863304122840773No Hit
CCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGG546810.2975224040937638No Hit
CGTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATA544520.2962764021819942No Hit
ATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTA528480.2875489477432239No Hit
CTCAATCATTTTCAGGAACGAGTGAAAGAATCATAGTGTCAACCACTCAA509420.27717829427670515No Hit
CTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCTTTAATT487740.26538208403776875No Hit
AAAAAATTGAAGCAGATTAAATCAGTCAATTAAAGGCCAAAGAAATTTGA476270.25914119236615435No Hit
TTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCT466770.2539721888020448No Hit
TTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTC457860.2491242075645483No Hit
ATTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAATCTG457570.24896641692943342No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA456940.2486236303772872No Hit
CAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAAAAAAATT448740.24416196414300315No Hit
ATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTG431300.23467276181057461No Hit
CTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGA418930.22794217506446565No Hit
AAATAATTGATTGACAGGCCGGGAAAACAATCTGCAACAAGTCAGATCTT418250.22757218323040304No Hit
GAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAAT412540.22446534003555405No Hit
CTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAAAAAAATTGAA393360.21402939389243597No Hit
GAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATG380050.20678734784629926No Hit
CCGGCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGA376360.20477959804087145No Hit
GCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGATTT360830.19632963747764814No Hit
GTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAA354450.19285824350511982No Hit
ATCATTTTCAGGAACGAGTGAAAGAATCATAGTGTCAACCACTCAATAAC332610.18097497636405108No Hit
AGAAATTTGAGTAAAATCAAATTATAATGAAATTAAGATAAAAGGGTAAC331210.1802132284703928No Hit
GAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAA326110.17743828971492345No Hit
CCGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCTGACTTGT312920.17026153634538607No Hit
GGGATATCCGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCT303650.1652176770780918No Hit
CGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCTGACTTGTT301290.1639335877716393No Hit
AACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGG300760.1636452117833258No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA300760.1636452117833258No Hit
CTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGATTATTTCGTTC300140.1633078662875629No Hit
TTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAATCTGC294170.16005955562674876No Hit
GGTACATTGATTTGTTTGCCAGCGTCGTAATTGTTATTGAGTGGTTGACA286200.15572303368927998No Hit
TATAATTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAA283950.15449879600304353No Hit
GGGTAACCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGA276700.15055403012517043No Hit
CCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACA274020.14909582701445317No Hit
AAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAATT273350.14873127623677387No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA273310.14870951201124077No Hit
CTCTTTTCTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTAC272520.1482796685569622No Hit
GAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTC266690.1451075326855139No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC264950.14416078887482436No Hit
TACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTT261260.14215303906939653No Hit
TCCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAA255920.13924751496072865No Hit
AAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGT252680.1374846126925481No Hit
GCCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTG249890.13596655796161486No Hit
CTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAATG248660.13529730802647225No Hit
CCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAG247330.13457364752749693No Hit
CATAACTTGGCGTATTGGTGTGAACGACCAATTTGCCTTCAGGTACTTTC246470.13410571667853544No Hit
CGCCAACTTTTTGGAATCATCTTACTCTTTAGATCGGATTTTTTCTCATT242110.1317334160954283No Hit
AGATGAACGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAA241810.1315701844039301No Hit
CGGCGATTAAAAACATTTTTGATATTTACGACCCTGAAATCATTGCCGTT232740.12663514626430125No Hit
TGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAAT231500.12596045527277538No Hit
GCTCTTTTCTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTA228120.12412137821522903No Hit
GAACGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAG227430.12374594532478318No Hit
GCCAACTTTTTGGAATCATCTTACTCTTTAGATCGGATTTTTTCTCATTA227380.12371874004286681No Hit
GAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAATTGGAAAC226600.12329433764497151No Hit
GTCGGTACATTGATTTGTTTGCCAGCGTCGTAATTGTTATTGAGTGGTTG224570.12218980319916703No Hit
CTTTACTCGATACTTTTGATTTAAGAACTTTTTCTCGTTTCCAATTTTTA221900.12073704114483308No Hit
ATTATAATGAAATTAAGATAAAAGGGTAACCTCACAAAGAATTAAATGAA220620.12004058592777411No Hit
CGGCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGAT220240.11983382578520972No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT212520.11563333025732278No Hit
TCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAATTAAAGG210310.11443085679661942No Hit
AATTGATTGACAGGCCGGGAAAACAATCTGCAACAAGTCAGATCTTCCAA205660.11190076557839736No Hit
TAAAAGGGTAACCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATG204770.11141651156028602No Hit
GAAAAAGTTCTTAAATCAAAAGTATCGAGTAAAGAATCCAGTAAATAATG204170.11109004817728965No Hit
GAAATAATTGATTGACAGGCCGGGAAAACAATCTGCAACAAGTCAGATCT203510.1107309384559936No Hit
ATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAA202470.1101650685921332No Hit
ATTAAATCAGTCAATTAAAGGCCAAAGAAATTTGAGTAAAATCAAATTAT201370.10956655238997313No Hit
AAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGT200030.10883745083461452No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC198330.10791247124945808No Hit
TAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAA197600.10751527413347912No Hit
ATTTTCAGGAACGAGTGAAAGAATCATAGTGTCAACCACTCAATAACAAT196490.1069113168749358No Hit
CAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAG195640.10644882708235757No Hit
CGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGA195470.10635632912384192No Hit
CTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTACTTTTAAG188360.10248773803533465No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA188070.10232994740021974No Hit
GAAAACCATGTCCAATTAAAAATTGGAAACGAGAAAAAGTTCTTAAATCA185600.10098600647355123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)