FastQCFastQC Report
Fri 21 Jul 2023
Syr-MC1-big_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSyr-MC1-big_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18378784
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCG3905752.125140596896944No Hit
GTTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACT3130041.7030724121900556No Hit
TGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAA2738221.4898809409806437No Hit
CTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCC2293491.247900840447333No Hit
ATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATC2280451.2408057029235449No Hit
CTTTAATTGACTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGAT2020871.099566761326538No Hit
GATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAAT1957831.0652663418863837No Hit
CCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCAC1822170.9914529709908991No Hit
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT1609400.8756836143239944No Hit
CTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGGAT1098010.5974334319397845No Hit
GTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAA984110.5354597997343024No Hit
AAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAAT967350.5263405892359364No Hit
ATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATA948840.5162691938704976No Hit
TTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGA933350.5078409975328074No Hit
TCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCA928250.505066058777338No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG821740.4471133672390948No Hit
GGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTC816070.4440282882697789No Hit
GTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAA815150.44352771108251776No Hit
CCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAAT783680.42640470664435687No Hit
CAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAG707840.38513973503361265No Hit
ATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGATTATTTCGTTCATC660590.35943074362264665No Hit
GTGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAA650560.35397336407022356No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA620870.33781886766828534No Hit
CGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCA620350.3375359327363551No Hit
GTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCT557780.3034912429462145No Hit
CTCAATCATTTTCAGGAACGAGTGAAAGAATCATAGTGTCAACCACTCAA544240.2961240526032625No Hit
ATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTA543230.29557450590855194No Hit
CCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGG535970.2916242989742956No Hit
CGTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATA528420.2875163014049243No Hit
CTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCTTTAATT498340.2711496038040384No Hit
ATTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAATCTG485750.2642993138174974No Hit
TTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCT485350.26408167156216644No Hit
AAAAAATTGAAGCAGATTAAATCAGTCAATTAAAGGCCAAAGAAATTTGA482720.2626506737333656No Hit
TTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTC457670.24902082749326615No Hit
CTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGA443160.2411258546811367No Hit
CAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAAAAAAATT441660.2403096962236457No Hit
ATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTG431410.23473261343079063No Hit
CCGGCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGA427430.2325670729902479No Hit
GAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAAT427290.23249089820088206No Hit
AAATAATTGATTGACAGGCCGGGAAAACAATCTGCAACAAGTCAGATCTT420330.2287039229581239No Hit
CTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAAAAAAATTGAA405920.22086336070982715No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA402080.21877399505865025No Hit
GAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATG390430.21243516437213691No Hit
GCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGATTT380700.20714101651121206No Hit
GTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAA358830.1952414262009935No Hit
ATCATTTTCAGGAACGAGTGAAAGAATCATAGTGTCAACCACTCAATAAC355040.19317926583173295No Hit
GAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAA341110.18559987428983332No Hit
AGAAATTTGAGTAAAATCAAATTATAATGAAATTAAGATAAAAGGGTAAC336580.18313507574821056No Hit
CTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGATTATTTCGTTC329220.17913045825012144No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA310360.1688686259112681No Hit
CCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACA309550.168427900344223No Hit
CCGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCTGACTTGT308410.16780761991652984No Hit
TTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAATCTGC306810.1669370508952061No Hit
TATAATTTGATTTTACTCAAATTTCTTTGGCCTTTAATTGACTGATTTAA306170.16658882328667662No Hit
GGGATATCCGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCT300850.16369418129077526No Hit
AACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGG300830.16368329917800872No Hit
CGGCAGTTAAAAAGATTTCGCTTTTTAATAGTTGGAAGATCTGACTTGTT293120.15948824470650508No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGCTAATCTCGTAT292550.15917810449265848TruSeq Adapter, Index 4 (97% over 37bp)
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA290590.15811165744153693No Hit
GGGTAACCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGA288860.15717035468723065No Hit
CTCTTTTCTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTAC288750.15711050306701468No Hit
AAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAATT276800.15060844068900314No Hit
GGTACATTGATTTGTTTGCCAGCGTCGTAATTGTTATTGAGTGGTTGACA270220.14702822558880935No Hit
GAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTC270180.14700646136327628No Hit
TCCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAA265460.1444382827503713No Hit
CCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAG260570.1417776061789507No Hit
TACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTT259190.141026740398059No Hit
CTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAATG257090.1398841185575716No Hit
AAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGT253340.13784372241384416No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC251370.1367718343063393No Hit
CGCCAACTTTTTGGAATCATCTTACTCTTTAGATCGGATTTTTTCTCATT247620.13473143816261185No Hit
AGATGAACGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAA247160.13448114956898127No Hit
GCCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTG246620.13418733252428452No Hit
CATAACTTGGCGTATTGGTGTGAACGACCAATTTGCCTTCAGGTACTTTC242210.131787826659261No Hit
GCTCTTTTCTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTA238670.1298616926995823No Hit
ATTATAATGAAATTAAGATAAAAGGGTAACCTCACAAAGAATTAAATGAA233920.1272771909175275No Hit
GAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAATTAAAAATTGGAAAC232980.12676573161749982No Hit
GAACGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAG232190.12633588816322122No Hit
TGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAAT230230.1252694411120997No Hit
CTTTACTCGATACTTTTGATTTAAGAACTTTTTCTCGTTTCCAATTTTTA228520.12433902047055997No Hit
CGGCGATTAAAAACATTTTTGATATTTACGACCCTGAAATCATTGCCGTT225810.12286449419069292No Hit
TCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAATTAAAGG224010.12188510404170373No Hit
CGGCCTAGTCCAAAATCTCTGTTGTCATCCGTTTGGATTCCTCCTATGAT218030.11863135232450635No Hit
GCCAACTTTTTGGAATCATCTTACTCTTTAGATCGGATTTTTTCTCATTA217260.11821239098299431No Hit
TAAAAGGGTAACCTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATG216480.117787988585099No Hit
ATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAA213680.11626449279778248No Hit
ATTTTCAGGAACGAGTGAAAGAATCATAGTGTCAACCACTCAATAACAAT212800.11578567983605444No Hit
GTCGGTACATTGATTTGTTTGCCAGCGTCGTAATTGTTATTGAGTGGTTG212630.1156931818775388No Hit
ATTAAATCAGTCAATTAAAGGCCAAAGAAATTTGAGTAAAATCAAATTAT206620.11242310699119157No Hit
GAAATAATTGATTGACAGGCCGGGAAAACAATCTGCAACAAGTCAGATCT205790.11197149931137991No Hit
GAAAAAGTTCTTAAATCAAAAGTATCGAGTAAAGAATCCAGTAAATAATG204840.11145459895496895No Hit
AATTGATTGACAGGCCGGGAAAACAATCTGCAACAAGTCAGATCTTCCAA203940.11096490388047436No Hit
CAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGAAAAG203160.11054050148257905No Hit
CGATAAAAGAAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGA201220.10948493654422405No Hit
AAAAGTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGT200970.10934891013464221No Hit
TAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAA200610.10915303210484438No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT198410.10795599970052426No Hit
CTCACTTTTCAATGTAAATGTTCCCAACTTTTTTTGTTTTTACTTTTAAG194790.10598633728977934No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC192030.10448460572799594No Hit
GTATGAATGAAAAAAGAAAAGTTCTCACGGTTTCTGAAAACCATGTCCAA191380.10413093706308318No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG188120.10235715268213609No Hit
GTCCAATTAAAAATTGGAAACGAGAAAAAGTTCTTAAATCAAAAGTATCG187600.10207421775020589No Hit
GAAAACCATGTCCAATTAAAAATTGGAAACGAGAAAAAGTTCTTAAATCA186540.10149746577357893No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA185690.1010349759810007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)