FastQCFastQC Report
Fri 21 Jul 2023
MC3-big_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC3-big_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12346777
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT3551212.8762242972396765No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA1154120.9347540657776519No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG1101980.8925244215555201No Hit
TTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCG826970.6697861312308467No Hit
GTTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACT707260.5728296542490401No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA623990.5053869524006143No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA623690.5051439740103835No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA612210.49584600094421405No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC594830.48176945287017003No Hit
TGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAA539930.43730440745791394No Hit
CTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCC506540.4102609126252138No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT460330.37283414124997966No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA446680.36177862449447334No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC441000.3571782336394348No Hit
ATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATC439800.3562063200785112No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG422540.3422269633605596No Hit
GATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAAT400350.32425466176314677No Hit
CTTTAATTGACTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGAT375210.30389307266179666No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG361610.2928780523046622No Hit
CCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCAC360880.29228680488843367No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA348940.2826162649572435No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC348410.28218700313450223No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG300350.24326186501951075No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG288500.23366421860538988No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC261330.21165847573014399No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA256260.20755214093524163No Hit
GTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAA250560.20293555152085438No Hit
CTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGGAT238500.1931678202335719No Hit
ATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATA236970.19192863044339425No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA227340.18412902411698212No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT222580.18027376699198502No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG219710.17794927372544267No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC210100.17016586595837926No Hit
TTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGA208490.16886188193080673No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT204210.1653953902301791No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA202230.16379173285465512No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC194440.15748239398832586No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT193140.1564294876306586No Hit
TCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCA191920.15544137551038623No Hit
GGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTC190750.1544937597884857No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA189420.15341655559179532No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG189210.1532464707186337No Hit
AAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAAT187800.15210447228454843No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC185950.15060610554479117No Hit
CCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGGC184360.14931832007656737No Hit
GCCGAGTTCTGTTTAAATAGTCATCTATCTCGATTTATTGTTACCAATAA179120.14507429752720083No Hit
GTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAAAG176630.1430575768882843No Hit
GTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAA175560.14219095396312736No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG172330.13957488662830794No Hit
CCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAAT169460.1372503933617656No Hit
CTCATGAATAACTGTGTTTTATACTCTACCGAGTGCACTTTATCCTCTGC167620.13576012590168268No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA163400.13234222987910124No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC162490.13160519542873417No Hit
AAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTGTG157450.1275231584728549No Hit
GCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAA156260.1265593441916056No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC155940.126300167242026No Hit
CCGAAACCCAAAACCACAGGTTGAAATGGGAGGCGTGCAGCATTAATTAT150120.12158638647154638No Hit
CAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAG149660.12121381960652565No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT149160.12080885562280748No Hit
GGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGATCATCGTAAAAA147820.11972355214644274No Hit
CTCATGAATAACTGTGTTTTATACTCTACCGAGTGTAGTTTATCCTCTGC146140.11836287316114966No Hit
GAATAACTGTGTTTTATACTCTACCGAGTGTAGTTTATCCTCTGCCGAGC145270.11765823582948003No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA140620.11389207078090097No Hit
GAATAACTGTGTTTTATACTCTACCGAGTGCACTTTATCCTCTGCCGAGC139560.11303354713541841No Hit
TCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTA136520.11057136611441187No Hit
GTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGATCA135290.10957515471446518No Hit
TGAAGCTTGAGTAGCGAGTCGATGTTGCTGATCATCGTAAAAAGTGGCGC133750.10832786564461316No Hit
GTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCT133380.10802819229666172No Hit
CTCAATCCTTTTCCAGATTGACTGACACGGTCATTGAAAAGTAAATAAAC130090.1053635292837961No Hit
GCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAA129710.10505575665617026No Hit
CAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAA129590.10495856530007791No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC128500.10407574381557226No Hit
AAAACCTGTAAAACAGCCGAAACATCCGCGAGGGTGAATGCGGCTAAAAT125900.10196993110023772No Hit
GGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCT124750.10103851393768591No Hit
TGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAA124610.10092512402224484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)