FastQCFastQC Report
Fri 21 Jul 2023
MC2-big_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC2-big_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11437795
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAAT4047983.539126203958018No Hit
CGGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGA1161461.0154579619585768No Hit
TTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCG1053950.9214625721128942No Hit
GTTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACT964770.8434929984319529No Hit
CCCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGG854800.7473468443874016No Hit
CCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAA722600.6317651260579508No Hit
CTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCC666250.5824986371936199No Hit
GGAGAGTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAA653890.5716923585358891No Hit
TGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAA651440.5695503372809182No Hit
GGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTC636860.5568031250778669No Hit
ATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATC549310.48025865125227374No Hit
GCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTC528090.4617061243010563No Hit
GTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGA513810.4492212004149401No Hit
GATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAAT499930.4370859942847376No Hit
CGGGGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTT494280.43214623098245775No Hit
CCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCAC491020.42929603127176175No Hit
CTTTAATTGACTGATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGAT487850.42652451805614633No Hit
ATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAG478430.4182886649043806No Hit
CTGGTTTCGACGGGGGTTTTGAAGCTTGAGTAGCGAGTCGATGTTGCTGA474560.41490514561591635No Hit
GGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCC410810.35916887826718347No Hit
GTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCA401810.3513002287591271No Hit
GTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATCGAG393950.3444282748554245No Hit
CTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGG341890.2989125089232671No Hit
GGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGG336460.29416509038673977No Hit
GTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAA332020.29028322329609857No Hit
CTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAGGAT315860.2761546259571884No Hit
CACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCA297190.2598315496999203No Hit
CTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTC292290.2555475071899785No Hit
ATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATA286510.25049408561702674No Hit
TTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGA268610.2348442160398923No Hit
TCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCA264980.2316705274049762No Hit
GTTTGAGGTCTGCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAA257220.22488600294025204No Hit
TGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGG256350.22412536682113993No Hit
TTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGAT249170.21784793310249045No Hit
AAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCAAT239270.20919241864362842No Hit
GGTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTC237650.20777606173217827No Hit
GCCAGAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTAT235900.2062460465500562No Hit
GTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAA232750.20349201922223648No Hit
GTTAGCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCC221810.193927238597999No Hit
GCGGTGCTTTGTAACCTGCAATCCGCTGTAGCAGGGTTGAATTCCCCATC220770.19301797243262359No Hit
TGGACCCTTCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAG218150.1907273211313894No Hit
GTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACCCCGTCA216600.18937216482722413No Hit
TTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCT216580.18935467893942845No Hit
GAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGAC214270.1873350588990273No Hit
CCTCCTATGATTTTTTTATCAAAGCTCTTTTCTCACTTTTCAATGTAAAT213450.18661813749940437No Hit
TTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGA210270.18383788133989112No Hit
CAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTCACAG198370.173433778101461No Hit
CAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATC186080.16268870005101507No Hit
GCGGTAAGTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAA179110.15659486815422027No Hit
AAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACC178440.15600909091306497No Hit
AAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTGTG176780.15455776222602347No Hit
TCGCAATAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTA176330.15416432975062064No Hit
CTCCTGTCAGGTGGATTATTATCTTTGATCCATTATTCGTATTTATGGCC172430.15075458163046285No Hit
CGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGCTCAAATCAGATGA166050.14517658342364065No Hit
GTTACCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCT165550.14473943622874863No Hit
CGCTGCTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGG164030.1434105087562769No Hit
ATAAGAACCAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTA163520.14296461861748702No Hit
CTCAATCCTTTTCCAGATTGACTGACACGGTCATTGAAAAGTAAATAAAC163140.14263238674936907No Hit
GTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAAAG160880.14065648142845716No Hit
CGCCACTTTCCTCTGGCAGACCTCAAACCTTGGCGCCTCGATGGGGAATT154540.1351134549972263No Hit
CGTTCATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATA149440.13065455360932765No Hit
CTTCATTCCTGACCTGACGGGGTTCATAAGTTTCTATTGCGAAGGGTCCA149080.13033980762900543No Hit
GCCGAGTTCTGTTTAAATAGTCATCTATCTCGATTTATTGTTACCAATAA148940.13021740641443563No Hit
CCCTTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGG148150.12952671384650624No Hit
TTTTATCTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCT147260.12874859183959847No Hit
CCTGCTTCAATGAACGCCGAAACCCAAAACCACAGGTTGAAATGGGAGGC144970.126746457686993No Hit
CACCCCTTTCAATTAACCAAAAATCTTCTATTCGCTTTTCACAAGTAATA144090.12597707862398302No Hit
GTGATCATTTAAAAGTAAATAAGAACCAACTTGCCAATAAGATGAACGAA143800.12572353325094565No Hit
GTGCACCCCATTTCGACTACCAACTTCCAAGCGTTACACAGGCAGTTAGC141560.12376511381782938No Hit
CTTAATTTCATTATAATTTGATTTTACTCAAATTTCTTTGGCCTTTAATT134770.11782865491119572No Hit
CGAAATAATCGAGATCAACCTAAAAAAATTGAAGCAGATTAAATCAGTCA134720.11778494019170653No Hit
TAGAAACTTATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAG134510.11760133836985187No Hit
GAGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAAC134170.11730407827732531No Hit
ATTTAATCTGCTTCAATTTTTTTAGGTTGATCTCGATTATTTCGTTCATC132670.11599263669264924No Hit
GGTTCATAAGTTTCTATTGCGAAGGGTCCAACTCTCAACGCCACTTTCCT132600.11593143608536434No Hit
TTCCCAACTTTTTTTGTTTTTACTTTTAAGATTTGATTTGTTTTATACTC130620.11420033319359195No Hit
TATGAACCCCGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTA130590.11417410436189843No Hit
AAAAAATTGAAGCAGATTAAATCAGTCAATTAAAGGCCAAAGAAATTTGA129860.11353586945735607No Hit
GGCCACATCATTTTGTACTGTTAAATATTTGATGTTCTTTTTCCATAATG129610.11331729585991007No Hit
CCCCTTTCAATTAACCAAAAATCTTCTATTCGCTTTTCACAAGTAATAAA124810.10912068278894665No Hit
CTCACAAAGAATTAAATGAACGATAAAAGAAAAGTATGAATGAAAAAAGA123650.10810650129679715No Hit
GCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAA121130.10590327943454135No Hit
CAACTTGCCAATAAGATGAACGAAATAATCGAGATCAACCTAAAAAAATT120240.10512515742763356No Hit
CAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAAAA118670.10375251523567261No Hit
TGCAGTCATTGTTTATTTTCTCATTCCCCTCTTTCTCCAATGACATAGAA118400.1035164557504309No Hit
CTCATGAATAACTGTGTTTTATACTCTACCGAGTGCACTTTATCCTCTGC118290.10342028336755467No Hit
GTAAGACCTTCTATGTGCCGGAGGATCATCTGATTTGAGCTAACTGCCTG117810.10300062206045835No Hit
AGGAAAGTGGCGTTGAGAGTTGGACCCTTCGCAATAGAAACTTATGAACC117210.1024760454265879No Hit
CGTCAGGTCAGGAATGAAGCAGCGGTAAGTAAGACCTTCTATGTGCCGGA116930.10223124299744837No Hit
CCGAAACCCAAAACCACAGGTTGAAATGGGAGGCGTGCAGCATTAATTAT115290.10079740019820255No Hit
ATTTAATTCTTTGTGAGGTTACCCTTTTATCTTAATTTCATTATAATTTG115200.10071871370312198No Hit
ACTCAATCCTTTTCCAGATTGACTGACACGGTCATTGAAAAGTAAATAAA114980.10052636893736948No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)