A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
Report
generated on 2025-05-27, 14:17 CEST
based on data in:
/archiv/READS/Acetobacterium_dehalogenans_Kurth_2023_07_21/TODO/MAPPED
General Statistics
| Sample Name | Reads | Reads mapped | % Reads mapped | % Aligned |
|---|---|---|---|---|
| MC2-big | 25.7M | 25.6M | 99.5% | |
| MC2-big.err | 99.4% | |||
| MC3-big | 28.6M | 28.3M | 99.1% | |
| MC3-big.err | 98.9% | |||
| MC4-big | 28.6M | 28.4M | 99.3% | |
| MC4-big.err | 99.2% | |||
| Syr1 | 31.3M | 31.1M | 99.5% | |
| Syr1.err | 99.4% | |||
| Syr2 | 35.3M | 35.1M | 99.5% | |
| Syr2.err | 99.5% | |||
| Syr4 | 47.6M | 47.3M | 99.5% | |
| Syr4.err | 99.4% | |||
| Syr-MC1-big | 40.8M | 40.5M | 99.2% | |
| Syr-MC1-big.err | 99.1% | |||
| Syr-MC2-big | 21.8M | 21.6M | 99.2% | |
| Syr-MC2-big.err | 99.1% | |||
| Syr-MC3-big | 26.8M | 26.6M | 99.3% | |
| Syr-MC3-big.err | 99.2% |
Samtools
Toolkit for interacting with BAM/CRAM files.URL: http://www.htslib.orgDOI: 10.1093/bioinformatics/btp352
Flagstat
This module parses the output from samtools flagstat
Bowtie 2 / HiSAT2
Results from both Bowtie 2 and HISAT2, tools for aligning reads against a reference genome.URL: http://bowtie-bio.sourceforge.net/bowtie2; https://ccb.jhu.edu/software/hisat2DOI: 10.1038/nmeth.1923; 10.1038/nmeth.3317; 10.1038/s41587-019-0201-4
Paired-end alignments
This plot shows the number of reads aligning to the reference in different ways.
There are 6 possible types of alignment:
- PE mapped uniquely: Pair has only one occurence in the reference genome.
- PE mapped discordantly uniquely: Pair has only one occurence but not in proper pair.
- PE one mate mapped uniquely: One read of a pair has one occurence.
- PE multimapped: Pair has multiple occurence.
- PE one mate multimapped: One read of a pair has multiple occurence.
- PE neither mate aligned: Pair has no occurence.