Title of the dataset: BIONANO-MSCA4U. BIONANO-MSCA4U. Nutrient analysis and qPCR data of tomato plants treated with N5.12-AgNPs under drought 

Name/Initials of the main responsible person: Arslan Svitlana

Number of version: v1

Project research challenges with code: MSCA4Ukraine project (ID:101101923)

Type of data hosted (brief description): 

This dataset contains nutrient analysis and qPCR-based gene expression data investigating the impact of biosynthesized silver nanoparticles (N5.12-AgNPs) from Pseudomonas N5.12 on tomato plants exposed to drought stress. Tomato plants were cultivated under greenhouse conditions and pretreated with bacterial cells (Сells), bacterial filtrate (ML), and AgNPs (NP30 and NP60). Treatments were applied either via soil drench or foliar spray prior to the induction of drought stress. The analyzed genes included plasma membrane H⁺-ATPase1 (HA1), high-affinity nitrate transporter 2.2 (NRT2.2), phosphate overaccumulator 1 (PHO1), and Arabidopsis potassium channel 2/3 (AKT2/3). Actin and elongation factor 1-alpha (Ef1α) were used as housekeeping genes to normalize gene expression levels in quantitative PCR analyses. 

Application of N5.12-AgNPs enhanced drought tolerance and significantly increased leaf nutrient content (including N, P, K, Mg, S and Ca), compared with untreated plants. NP60 induced more pronounced and consistent nutrient accumulation than NP30, while ML improved N, P, K and Cells primarily enhanced S and Na. These effects may be linked to enhancement of photosynthetic activity, facilitating nutrient uptake and translocation. 

Gene expression analysis revealed that ML and NP60 treatments differentially modulated nutrient transporter genes in tomato leaves. ML induced early upregulation of HA1, PHO1, and AKT2/3 before rewatering, followed by a second peak at 24 hour. NP60 induced a more targeted response, with early AKT2/3 induction, followed by upregulation of NRT2.2, PHO1, and AKT2/3 at 14 h after rewatering. In summary, ML primarily promoted early stress-responsive mechanisms, particularly potassium transport and energy metabolism, whereas NP60 enhanced overall nutrient transport activation after rewatering. Therefore, N5.12-AgNPs can act not only as stress modulators but also as nanonutrient delivery systems, enhancing plant resilience.





