
# Cell-Cell Communication Analysis Report

## Dataset Summary
- **Total Cells**: 10,000
- **Total Genes**: 1,000
- **Cell Types Identified**: 10

## Cell Type Distribution
leiden
3    2548
6    1979
7    1194
0     948
4     681
1     676
2     649
9     626
8     579
5     120

## Quality Control Metrics
- **Mean genes per cell**: 714
- **Mean UMI per cell**: 1588
- **Mean mitochondrial %**: 0.21%

## Analysis Steps Completed
1. ✅ Data preprocessing and quality control
2. ✅ Cell type annotation and clustering
3. ✅ Cell-cell communication analysis
4. ✅ Visualization and reporting

## Files Generated
- `analysis_summary.png`: Overview of dataset characteristics
- `interactive_umap.html`: Interactive cell type visualization
- `communication_network.png`: Cell communication network
- `communication_heatmap.png`: Communication strength matrix

## Next Steps
1. Validate cell type annotations with known markers
2. Investigate specific ligand-receptor pairs of interest
3. Compare with other datasets or conditions
4. Perform pathway enrichment analysis
