<help text in angle brackets should be deleted before finalizing your document>
[text in square brackets should be changed for your dataset]

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GENERAL INFORMATION 
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This readme file was generated on [2025-09-19] by [Szu-Chi Liao]

Title of Dataset: CHCHD2 T61I Point Mutation
Description of Dataset: This is the dataset of the study "CHCHD2 Mutant Mice Link Mitochondrial Deficits to PD Pathophysiology"
Principal Investigator: Ken Nakamura, ken.nakamura@gladstone.ucsf.edu, 0000-0002-9192-182X
Date of Data Collection: 2018-08-30 to 2025-08-31 
Software Dependencies: Microsoft Excel

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FILE OVERVIEW 
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Directory of Files: 
Relationship Between Files: 
File Formats: Excel
File Naming Convention: 

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DATA SPECIFIC INFORMATION FOR Fig1B
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Date of Creation: 2023-10-24
Description of Data: Representative genotyping result of CHCHD2 T61I point-mutant mice
Number of Variables: 2
Variable List:
	A. MT signal (MT, mutant)
	B. WT signal (WT, wild-type)

Number of Rows: 71
Column Names:
	A. Mouse_ID, for individual mouse identification
	B. MT signal, fluorescence signal from the mutant probe after PCR amplification
	C. WT signal, fluorescence signal from the wild-type probe after PCR amplification
	D. Genotype, based on the signals from MT and WT

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DATA SPECIFIC INFORMATION FOR Fig1D
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Date of Creation: 2023-10-23
Description of Data: Track of CHCHD2 T61I mice body weight
Number of Variables: 3
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Sex (F, female; M, male)
	Description: the gender of the mouse
	C. Age (mo, months)
	Description: from 1 month to 13 months
	
Number of Rows: 39
Column Names:
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Sex, M or F
	D. 1mo, bodyweight (g) at 1 month
	E. 2mo, bodyweight (g) at 2 month
	F. 3mo, bodyweight (g) at 3 month
	G. 4mo, bodyweight (g) at 4 month
	H. 7mo, bodyweight (g) at 7 month
	I. 13mo, bodyweight (g) at 13 month

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DATA SPECIFIC INFORMATION FOR Fig1E
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Date of Creation: 2024-03-28
Description of Data: Exhibition of the survival curve of CHCHD2 T61I point-mutant mice
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	Month, live or dead
	
Number of Rows: 137
Column Names:
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Age (Month)
	D. Event, if live, =0; =1 if dead

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DATA SPECIFIC INFORMATION FOR Fig1F
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Date of Creation: 2022-09-21
Description of Data: Total distanse traveled in open field test
Number of Variables: 3
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Sex (F, female; M, male)
	Description: the gender of the mouse
	C. Age (months)
	Description: 3 time points at 3, 7 and 12 months

Number of Rows: 283
Column Names:
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Sex, M or F
	D. Age, months
	E. Distance traveled, cm

Missing data codes: 
	A. NA, not collected

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DATA SPECIFIC INFORMATION FOR Fig1G
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Date of Creation: 2022-09-21
Description of Data: Latency to fall in inverted grid hang test
Number of Variables: 3
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Sex (F, female; M, male)
	Description: the gender of the mouse
	C. Age (months)
	Description: 3 time points at 3, 7 and 12 months

Number of Rows: 283
Column Names:
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Sex, M or F
	D. Age, months
	E. Latency to fall, s

Missing data codes: 
	A. NA, not collected

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DATA SPECIFIC INFORMATION FOR Fig1H
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Date of Creation: 2022-09-21
Description of Data: Latency to fall in rotarod test
Number of Variables: 3
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Sex (F, female; M, male)
	Description: the gender of the mouse
	C. Age (months)
	Description: 3 time points at 3, 7 and 12 months

Number of Rows: 280
Column Names:
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Sex, M or F
	D. Age, months
	E. Latency to fall, s

Missing data codes: 
	A. NA, not collected

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DATA SPECIFIC INFORMATION FOR Fig1I
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Date of Creation: 2022-09-21
Description of Data: Time spent in the open arm in elevated plus maze test
Number of Variables: 3
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Sex (F, female; M, male)
	Description: the gender of the mouse
	C. Age (months)
	Description: 3 time points at 3, 7 and 12 months

Number of Rows: 283
Column Names:
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Sex, M or F
	D. Age, months
	E. Time in open arm, %

Missing data codes: 
	A. NA, not collected

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DATA SPECIFIC INFORMATION FOR Fig2A-I
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Date of Creation: 2024-03-19
Description of Data: Latency to fall in rotarod test
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation

Number of Rows: 34
Column Names: Cell number, Mouse number, Genotype, Th, M-L, A-P, Spine, Ih magnitude, AP threshold, AP duration, AP peak, Cell attached FR, ISI-CV, Whole cell FR, ISI_CV, Ri, Baseline, Quinpirole, Change
	A. Cell number, individual cell tested
	B. Mouse number, mouse tested
	C. Genotype, WT or HOM
	D. Th
	E. M-L
	F. A-P
	G. Spine
	H. Ih magnitude
	I. AP threshold
	J. AP duration
	K. AP peak
	L. Cell attached FR
	M. ISI-CV
	N. Whole cell FR
	O. ISI_CV
	P. Ri
	Q. Baseline
	R. Quinpirole
	S. Change

Missing data codes: 
	A. NA, not collected


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DATA SPECIFIC INFORMATION FOR Fig2J
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Date of Creation: 2024-08-27
Description of Data: Catecholamine levels in CHCHD2 T61I mice
Number of Variables: 2
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Age (months)
	Description: 2 time points at 5 and 16 months
	
Number of Rows: 26
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Age, months
	C. Genotype, WT or HOM
	D. DA level, dopamine level, ng/mg protein
	E. DOPAC level, 3,4-hyroxyphenylacetic acid, ng/mg protein
	F. HVA level, homovanillic acid level, ng/mg protein

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DATA SPECIFIC INFORMATION FOR Fig2K
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Date of Creation: 2023-12-21
Description of Data: Opstical density of tyrosine hydroxylase staining at DA neuron terminal
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 10
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. CPu, subregion of caudate putamen, relative intensity
	D. NAc-C, subregion of nucleus accumbens core, relative intensity
	E. NAc-Sh, subregion of nucleus accumbens shell, relative intensity
	F. OT, subregion of olfactory tubercle, relative intensity

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DATA SPECIFIC INFORMATION FOR Fig2L
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Date of Creation: 2023-12-21
Description of Data: Dopamine neuron quantification by stereology
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 19
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. SN count, number of dopaminergic neurons in the subregion subtantia nigra
	D. VTA count, number of dopaminergic neurons in the ventral tagmental area

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DATA SPECIFIC INFORMATION FOR Fig3B
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Date of Creation: 2024-01-24
Description of Data: Quantification of CHCHD2 and CHCHD10 by immunofluorescence staining
Number of Variables: 2
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Subregion (SN, substantia nigra; VTA, ventral tagmental area)
	Description: Subregions in the midbrain with different subtypes of dopaminergic neurons
	
Number of Rows: 49
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Region, SN or VTA
	D. CHCHD2 intensity, relative intensity
	E. CHCHD10 intensity, relative intensity
	F. CHCHD2 Area %, punctae area normalized to cell area
	G. CHCHD10 Area %, punctae area normalized to cell area
	H. D2/D10 colocalization, over-lapping area of CHCHD2 and CHCHD10 punctae

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DATA SPECIFIC INFORMATION FOR Fig3C
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Date of Creation: 2025-03-18
Description of Data: Quantification of CHCHD2 and CHCHD10 by immunofluorescence staining
Number of Variables: 2
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Subregion (SN, substantia nigra; VTA, ventral tagmental area)
	Description: Subregions in the midbrain with different subtypes of dopaminergic neurons
	
Number of Rows: 42
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Region, SN or VTA
	D. CHCHD2 Area %, punctae area normalized to cell area
	E. CHCHD10 Area %, punctae area normalized to cell area
	F. D2/D10 colocalization, over-lapping area of CHCHD2 and CHCHD10 punctae

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DATA SPECIFIC INFORMATION FOR Fig3E
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Date of Creation: 2024-01-24
escription of Data: Quantification of CHCHD2 and mitochondrial marker (PDH) by immunofluorescence staining
Number of Variables: 2
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Subregion (SN, substantia nigra; VTA, ventral tagmental area)
	Description: Subregions in the midbrain with different subtypes of dopaminergic neurons
	
Number of Rows: 49
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Region, SN or VTA
	D. Normalized cell area
	E. Mitochondria, mitochondrial area determined by PDH signal
	F. Percentage of CHCHD2 punctae in mitochondria, punctae area in mitochondria
	G. Percentage of CHCHD10 punctae in mitochondria, punctae area in mitochondria

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DATA SPECIFIC INFORMATION FOR Fig3F
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Date of Creation: 2023-10-25
Description of Data: Relative abundance of CHCHD2 in soluable/insoluble fractions of cytoplasmic or mitochondrial extract
Number of Variables: 2
Variable List:
	A. Genotype (WT, wild-type; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Fraction (Cytosolic; Mitochondrial)
	Description: Cytosolic or mitochondrial brain lysate
	
Number of Rows: 13
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT or HOM
	C. Fraction, cytosolic or mitochondrial
	D. Relative Level, band intensity of insoluble fraction to soluble

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DATA SPECIFIC INFORMATION FOR Fig3H
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Date of Creation: 2024-07-26
Description of Data: Quantification of mitochondria distribution by morphology
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 7
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT or HOM
	C. % Normal, percentage of mitochondria in normal form
	D. % Vesicular, percentage of mitochondria in vesicular form
	E. % Swollen, percentage of mitochondria in swollen form

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DATA SPECIFIC INFORMATION FOR Fig4A
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Date of Creation: 2023-12-19
Description of Data: Expression of CHCHD2 and CHCHD10 in dopaminergic neurons in human samples
Number of Variables: 3
Variable List:
	A. Disease group (PD, Parkinson's disease)
	Description: Human samples of health people or patients with incidental lewy body, early parkinson's or late parkinson's
	B. Gene
	Description: expression of CHCHD2 or CHCHD10
	C. Region (SND, substantia nigra dorsal area; SNV, substantia nigra ventral area)
	Description: expression in subregions of SND or SNV

Number of Rows: 17
Column Names: 
	A. Group, human samples from 4 different disease groups
	B. Gene, CHCHD2 or CHCHD10 expression
	C. Region, SND or SNV
	D. Expression, average expression level
	E. SD, standard diviation
	F. N, number of samples in each condition

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DATA SPECIFIC INFORMATION FOR Fig4B
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Date of Creation: 2023-12-19
Description of Data: Correlation of a-synucelin versus CHCHD10 or CHCHD2 gene expression
Number of Variables: 0
Variable List:
Number of Rows: 229
Column Names: 
	A. DA neuron_ID, each dopaminergic neuron analyzed
	B. SNCA, expression level of a-synuclein by spatial transcriptomics
	C. CHCHD10, expression level of CHCHD10 by spatial transcriptomics
	D. CHCHD2, expression level of CHCHD2 by spatial transcriptomics

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DATA SPECIFIC INFORMATION FOR Fig4G
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Date of Creation: 2023-12-17
Description of Data: Quantification of a-synuclein and phosphorylated a-synucelin level in the midbrain
Number of Variables: 2
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Subregion (SN, substantia nigra; VTA, ventral tagmental area)
	Description: Subregions in the midbrain with different subtypes of dopaminergic neurons

Number of Rows: 25
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Region, SN or VTA
	D. Syn1, expression level of a-synuclein, relative intensity
	E. pSyn, expression level of phosphorylated a-synuclein, relative intensity

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DATA SPECIFIC INFORMATION FOR Fig4H
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Date of Creation: 2023-12-17
Description of Data: Quantification of a-synuclein and phosphorylated a-synucelin level at dopaminergic neuron terminal in the striatum
Number of Variables: 2
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Subregion (CPu, caudate putamen; NAc-C, nucleus accumbens core; NAc-Sh, nucleus accumbens shell; OT, olfactory tubercle)
	Description: Subregions in the straitum representating axonal terminal of different dopaminergic neuron subtype
	
Number of Rows: 49
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Region, CPu, NAc-C, NAc-Sh or OT
	D. Syn1, expression level of a-synuclein, relative intensity
	E. pSyn, expression level of phosphorylated a-synuclein, relative intensity

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DATA SPECIFIC INFORMATION FOR Fig4I
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Date of Creation: 2025-08-28
Description of Data: Quantification of a-synuclein and phosphorylated a-synucelin level in cortex extracts
Number of Variables: 3
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Age (9, 17 or 23 months)
	Description: Cortex extract from mice at different ages
	C. Fractions (Triton; SDS)
	Description: Triton represents soluble fraction, and SDS represents insoluble fraction
	
Number of Rows: 65
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Age, 9, 17, or 23
	D. Fraction, Triton or SDS
	E. Syn1, level of a-synuclein protein, relative intensity
	F. pSyn, level of phosphorylated a-synuclein protein, relative intensity
	G. pSyn/Syn1, ratio of phosphorylated a-synuclein to total a-synuclein level

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DATA SPECIFIC INFORMATION FOR Fig5A
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Date of Creation: 2023-11-26
Description of Data: Relative amount or labeling percentage quantified by infusing 13C glucose into mice in Metabolomics
Variable List: 1
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 15
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. G6P-amount
	D. F6P-amount
	E. F1,6BP-amount
	F. 1,3BPG-amount
	G. 3PG-amount
	H. PEP-amount
	I. Pyr-amount
	J. Lac-amount
	K. 6P-gluconate-amount
	L. R5P-amount
	M. S7P-amount
	N. Cit-amount
	O. Aconitate-amount
	P. iCit-amount
	Q. a-KG-amount
	R. Succ-amount
	S. Fum-amount
	T. Mal-amount
	U. G6P-labeling
	V. F6P-labeling
	W. F1,6BP-labeling
	X. 1,3BPG-labeling
	Y. 3PG-labeling
	Z. PEP-labeling
	AA. Pyr-labeling
	AB. Lac-labeling
	AC. 6P-gluconate-labeling
	AD. R5P-labeling
	AE. S7P-labeling
	AF. Cit-labeling
	AG. Aconitate-labeling
	AH. iCit-labeling
	AI. a-KG-labeling
	AJ. Succ-labeling
	AK. Fum-labeling
	AL. Mal-labeling

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DATA SPECIFIC INFORMATION FOR Fig5B
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Date of Creation: 2023-11-26
Description of Data: Relative amount quantified by infusing 13C glucose into mice in Metabolomics
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 15
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. G6P
	D. F6P
	E. F1,6BP
	F. 1,3BPG
	G. 3PG
	H. PEP
	I. Pyr
	J. Lac
	K. 6P-gluconate
	L. R5P
	M. S7P
	N. Cit
	O. Aconitate
	P. iCit
	Q. a-KG
	R. Succ
	S. Fum
	T. Mal

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DATA SPECIFIC INFORMATION FOR Fig5C
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Date of Creation: 2023-11-26
Description of Data: Relative labeling quantified by infusing 13C glucose into mice in Metabolomics
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 15
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. G6P
	D. F6P
	E. F1,6BP
	F. 1,3BPG
	G. 3PG
	H. PEP
	I. Pyr
	J. Lac
	K. 6P-gluconate
	L. R5P
	M. S7P
	N. Cit
	O. Aconitate
	P. iCit
	Q. a-KG
	R. Succ
	S. Fum
	T. Mal

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DATA SPECIFIC INFORMATION FOR Fig5D
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Date of Creation: 2024-08-05
Description of Data: Respiratory exchange ratio measured by comprehensive lab animal monitoring system
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 23
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C-CT. Hour 1-96

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DATA SPECIFIC INFORMATION FOR Fig6C_WCL
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Date of Creation: 2023-11-21
Description of Data: Co-elution of proteins in whole cell lysate in Proteomics
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows:9896

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DATA SPECIFIC INFORMATION FOR Fig6C_Mt
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Date of Creation: 2023-11-21
Description of Data: Co-elution of proteins in mitochondrial fraction in Proteomics
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows:9896

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DATA SPECIFIC INFORMATION FOR Fig7A-B
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Date of Creation: 2023-11-21
Description of Data: Protein complexes detected by Proteomics in whole cell lysate
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 100

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DATA SPECIFIC INFORMATION FOR Fig7C-D
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Date of Creation: 2023-11-21
Description of Data: Protein complexes detected by Proteomics in the mitochondrial fraction
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 107

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DATA SPECIFIC INFORMATION FOR Fig8A
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Date of Creation: 2024-06-19
Description of Data: Complex I activity
Number of Variables: 2
Variable List:
	A. Genotype (WT, wild-type; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Age (months)
	Description: 2 time points at 5 and 11 months

Number of Rows: 17
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT or HOM
	C. Age, months
	D-CP. Time point at 0 to 45 minutes

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DATA SPECIFIC INFORMATION FOR Fig8B
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Date of Creation: 2025-07-18
Description of Data: ROS level in isolated mitochondria
Number of Variables: 2
Variable List:
	A. Genotype (WT, wild-type; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Age (months)
	Description: 2 time points at 5 and 11 months

Number of Rows: 20
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT or HOM
	C. Age, months
	D-AW. Time point at 0 to 45 minutes

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DATA SPECIFIC INFORMATION FOR Fig8C
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Date of Creation: 2023-11-21
Description of Data: Protein-protein interactions between ROS-associated proteins detected by Proteomics in the mitochondrial fraction
Number of Variables: 0
Variable List: NA
Number of Rows: 644

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DATA SPECIFIC INFORMATION FOR FigS1A
----------------------------------------
Date of Creation: 2024-03-28
Description of Data: Exhibition of the survival curve of CHCHD2 T61I point-mutant mice
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	Month, live or dead
	
Number of Rows: 33
Column Names:
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Age (Month)
	D. Event, if live, =0; =1 if dead

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DATA SPECIFIC INFORMATION FOR FigS1C
----------------------------------------
Date of Creation: 2022-09-21
Description of Data: Mouse movement in open field test
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 11
Column Names:
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Distance traveled, cm
	D. Moving time, s
	E. Non-moving time, s

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DATA SPECIFIC INFORMATION FOR FigS1D
----------------------------------------
Date of Creation: 2022-09-21
Description of Data: Mouse movement in elevated plus maze test
Number of Variables: 3
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Sex (F, female; M, male)
	Description: the gender of the mouse
	C. Age (months)
	Description: 3 time points at 3, 7 and 12 months

Number of Rows: 283
Column Names:
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Sex, M or F
	D. Age, months
	E. Open Arm/Total Distance, %
	F. Open Arm Entries
	G. Close Arm Entries

Missing data codes: 
	A. NA, not collected

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DATA SPECIFIC INFORMATION FOR FigS2D
----------------------------------------
Date of Creation: 2023-12-21
Description of Data: Opstical density of tyrosine hydroxylase staining at DA neuron terminal
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 9
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT or HOM
	C. CPu, subregion of caudate putamen, relative intensity
	D. NAc-C, subregion of nucleus accumbens core, relative intensity
	E. NAc-Sh, subregion of nucleus accumbens shell, relative intensity
	F. OT, subregion of olfactory tubercle, relative intensity

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DATA SPECIFIC INFORMATION FOR FigS3A
----------------------------------------
Date of Creation: 2025-01-20
Description of Data: Correlation of CHCHD2/CHCHD10 with other genes
Number of Variables: 2
Variable List:
	A. Region (SNV, ventral tier of Substantia Nigra; SND, dorsal tier of Substantia Nigra)
	Description: Different subpoplation of dopaminergic neurons
	B. Correlation Gene (CHCHD2; CHCHD10)
	Description: two genes of interest
	
Number of Rows: 36045
Column Names: 
	A. Region, SNV or SND
	B. Gene, CHCHD2 or CHCHD10
	C. Gene Group, OXPHOS gene or other
	D. Correlation value, the correlation between CHCHD2 or CHCHD10 to the gene

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DATA SPECIFIC INFORMATION FOR FigS3F
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Date of Creation: 2025-01-29
Description of Data: CHCHD2 or CHCH10 protein inclusion in Lewy aggregates
Number of Variables: 2
Variable List:
	A. Lwey Aggregation Stage (1; 2; 3; 4-core; 4-rim)
	Description: Different development stage of Lewy aggregates
	B. Protein (CHCHD2; CHCHD10)
	Description: two protein of interest
	
Number of Rows: 11
Column Names: 
	A. Lewy aggregation stage, 1, 2, 3, 4-core, 4-rim
	B. Protein, CHCHD2 or CHCHD10
	C. Average Intensity, normalized fluorescence intensity
	D. SD, standard deviation

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DATA SPECIFIC INFORMATION FOR FigS4
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Date of Creation: 2024-08-05
Description of Data: Energy expenditure measured by comprehensive lab animal monitoring system
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 22
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C-CT. Hour 1-96

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DATA SPECIFIC INFORMATION FOR FigS5&6
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Date of Creation: 2024-04-01
Description of Data: Gene expression in different subregions of dopaminergic neurons by Visium spatial transcriptomics
Number of Variables: 2
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Region (SN, substantia; VTA, ventral tagmental area)
	Description: subpopulations of dopaminergic neurons
	
Number of Rows: 47429
Column Names: 
	A. FeatureID, ID of gene detected and quantified
	B. FeatureName, name of gene detected and quantified
	C-P. Each mouse in the analysis

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DATA SPECIFIC INFORMATION FOR FigS8F&G
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Date of Creation: 2025-02-21
Description of Data: Validation of altered protein-protein interaction by co-immunoprecipitation followed by western blotting
Number of Variables: 1
Variable List:
	A. Genotype (WT, wild-type; HET, heterozygous; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	
Number of Rows: 12
Column Names: 
	A. IP, target protein of immunoprecipitation pulldown
	B. IB, target protein detected by western blotting
	C. WT_input, level of protein input in the WT group
	D. PM_input, level of protein input in the HOM group
	E. KO_input, level of protein input in the KO group
	F. WT_IP, level of protein pulled down by co-immunoprecipitation in the WT group
	G. PM_IP, level of protein pulled down by co-immunoprecipitation in the HOM group
	H. KO_IP, level of protein pulled down by co-immunoprecipitation in the KO group
	I. Normalized_WT_IP, normalized level of WT_IP to WT_input
	J. Normalized_PM_IP, normalized level of PM_IP to PM_input
	K. Normalized_KO_IP, normalized level of KO_IP to KO_input

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DATA SPECIFIC INFORMATION FOR FigS9
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Date of Creation: 2025-02-10
Description of Data: Evaluation of respiratory chain supercomplexes by blue native PAGE
Number of Variables: 2
Variable List:
	A. Genotype (WT, wild-type; HOM, homozygous)
	Description: Mice with or wihout the point-mutation
	B. Staining (Coomassie; OXPHOS cocktail)
	Description: staining by coomassie blue probing total level or by OXPHOS cocktail for specific respiratory chain components
	
Number of Rows: 19
Column Names: 
	A. Mouse_ID, for individual mouse identification
	B. Genotype, WT, HET or HOM
	C. Staining, Coomassie or OXPHOS cocktail
	D. Normalized Level, normalized supercomplex level to total protein amount

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METHODOLOGY
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Description of methods used for data collection: <include instruments and software used to collect data>
Weighing - scale
Behavioral analyses - open field, inverted grid hang, rotarod, elevated plus maze
Electrophysiology
Immunofluorescence and immunohistochemical staining - cell profiler
Western blotting
Metabolomics
Visium spatial transcriptomics
Proteomics
Complex I activity assay - NADH consumption
ROS level assay - Amplex Red

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DATA ACCESS AND SHARING
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Publications based on this dataset: CHCHD2 Mutant Mice Link Mitochondrial Deficits to PD Pathophysiology
Recommended citation for this dataset:
License information:


