# 📊 Título del Dataset

Datos de la publicación 
María Barco-Antoñanzas, Miriam Gil-Monreal, Mikel V. Eceiza, Mercedes Royuela, Ana Zabalza, Primary metabolism in an Amaranthus palmeri population with multiple resistance to glyphosate and pyrithiobac herbicides, Plant Science, Volume 318, 2022, 111212,

https://doi.org/10.1016/j.plantsci.2022.111212.
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## 🗂️ Estructura del Repositorio


- `data/` – Archivos de datos en formatos  `.csv`.
- `README.md` – Descripción del repositorio y guía de uso.

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## 📑 Descripción del Conjunto de Datos

- **Se aportan los mecanismos moleculares de la resistencia múltiple de una población a glifosato y piritiobac: copias génicas y mutaciones. Se ha caracterizado fisiológicamente las poblaciones mediante el contenido de siquimato, aminoácidos y carbohidratos, y niveles de expresión de los genes de los enzimas de las rutas de biosíntesis de los aminoácidos ramificados y aromáticos. 
9 ficheros csv  que se corresponden con las siguientes figuras en la publicación; con la siguiente identificación:

-rftable_ALSmutations---Table 3
-EPSPScopynumber_shikimate----Fig 3
-correlation_glyphosateshikimate----Fig 3
-table_ALSinvitro----Fig 4
-RNAselectedforgraphs_t1---Fig, 5 , 6 7
-total free, BCAA,AAA%---Fig 8
-totalsolublesugars---Fig 9
-starch---Fig 9

## 🧾 Resumen publicación asociada 

The objective of this work was to characterize the resistance mechanisms and the primary metabolism of a multiple resistant (MR) population of Amaranthus palmeri to glyphosate and to the acetolactate synthase (ALS) inhibitor pyrithiobac. All MR plants analysed were glyphosate-resistant due to 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene amplification. Resistance to pyrithiobac was more variable among individuals and was related to point mutations at five positions in the ALS gene sequence: A122, A205, W574, S653 and G654. All MR plants were heterozygous for W574, the most abundant mutation. In nontreated plants, the presence of mutations did not affect ALS functionality, and plants with the W574L mutation showed the highest ALS resistance level to pyrithiobac. The accumulation of the transcripts corresponding to several genes of the aromatic amino acid (AAA) and branched-chain amino acid (BCAA) pathways detected in nontreated MR plants indicated additional effects of EPSPS gene amplification and ALS mutations. The physiological performance of the MR population after treatment with glyphosate and/or pyrithiobac was compared with that of a sensitive (S) population. The increase induced in total soluble sugars, AAA or BCAA content by both herbicides was higher in the S population than in the MR population. Physiological effects were not exacerbated after the mixture of both herbicides in S or in MR populations. This study provides new insights into the physiology of a multiple resistant A. palmeri, which could be very useful for achieving effective management of this weed.
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## 📦 Formato y Tamaño

- **Formato(s):** `.csv`, `
- **Separador (si aplica):** `,` (coma), `.
- **Codificación:** UTF-8
- **Tamaño total del repositorio:** [Ej: menos a 1 MB]

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