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GENERAL INFORMATION 
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This readme file was generated on 2025-05-21 by Eddy Albarran

Title of Dataset: Source Data for Albarran et al.
Description of Dataset: This dataset includes the source data for outputs from electrophysiology recordings that were used in the summary statistics and comparisons in the figures of manuscript. In addition, it includes a table of the genetic sequence information used in the generation and characterization of the knock-in lines.
Principal Investigator: Rui M. Costa (https://orcid.org/0000-0003-0495-8374)
Date of Data Collection: June 2021 to May 2025
Software Dependencies: Microsoft Excel

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FILE OVERVIEW 
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Directory of Files: 'SOURCE_DATA_FIG_4.xlsx', 'SOURCE_DATA_FIG_5.xlsx', 'SOURCE_DATA_FIG_6.xlsx', 'SEQUENCES.xlsx', 
Relationship Between Files: No relationship.
File Formats: .xlsx
File Naming Convention: If the file has FIG_X in the name, it contains the source data corresponding to Figure X in the manuscript. The 'SEQUENCES' file is a standalone file.

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DATA SPECIFIC INFORMATION FOR 'SOURCE_DATA_FIG_4.xlsx'
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Date of Creation: 2025-05-20
Description of Data: Electrophysiological parameters from whole-cell recordings + current injections from striatal D1-Cre and A2a-Cre neurons.
Number of Variables: 4
Variable List:
	A. Number of action potentials in response to current injected (f-I)
	Description: Each recorded neuron's number of action potentials evoked by a set amount of current injected through the electrode.
	# action potentials (integer values)
	
	B. Voltage-current relationship (I-V)
	Description: The membrane voltage in response to injected current values.
	mV

	C. Resting-membrane potential (RMP)
	Description: The membrane potential without any current injected
	mV
	
	D. Rheobase current (I_rheobase)
	Description: The amount of current needed to evoke a single action potential
	pA
Number of Rows: 78
Column Names: 
	A. MOUSE_ID: the identifier of the mouse recorded
	integer
	B. GENOTYPE: the genotype of the mouse recorded
	txt
	C. tdTom+/-: whether the cell recorded was positive or negative for tdTomato expression
	+ (positive), or - (negative)
	D. I_inj:X: the response to X pA of current injected
	varies by sheet (i.e., for sheet 'f-I' it corresponds to #action_potentials)
	E. RMP: resting membrane potential for this cell
	mV
	F. I_rheobase: the rheobase recorded for this cell
	pA
Missing data codes:
	A. NaN: could not extract a value from the recorded trace (i.e., the current injection was so large that the voltage response trace was too large in amplitude to isolate action potentials)

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DATA SPECIFIC INFORMATION FOR 'SOURCE_DATA_FIG_5.xlsx'
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Date of Creation: 2025-05-20
Description of Data: Electrophysiological parameters from whole-cell recordings + current injections from striatal D1-FlpO and A2a-FlpO neurons.
Number of Variables: 4
Variable List:
	A. Number of action potentials in response to current injected (f-I)
	Description: Each recorded neuron's number of action potentials evoked by a set amount of current injected through the electrode.
	# action potentials (integer values)
	
	B. Voltage-current relationship (I-V)
	Description: The membrane voltage in response to injected current values.
	mV

	C. Resting-membrane potential (RMP)
	Description: The membrane potential without any current injected
	mV
	
	D. Rheobase current (I_rheobase)
	Description: The amount of current needed to evoke a single action potential
	pA
Number of Rows: 78
Column Names: 
	A. MOUSE_ID: the identifier of the mouse recorded
	integer
	B. GENOTYPE: the genotype of the mouse recorded
	txt
	C. tdTom+/-: whether the cell recorded was positive or negative for tdTomato expression
	+ (positive), or - (negative)
	D. I_inj:X: the response to X pA of current injected
	varies by sheet (i.e., for sheet 'f-I' it corresponds to #action_potentials)
	E. RMP: resting membrane potential for this cell
	mV
	F. I_rheobase: the rheobase recorded for this cell
	pA
Missing data codes:
	A. NaN: could not extract a value from the recorded trace (i.e., the current injection was so large that the voltage response trace was too large in amplitude to isolate action potentials)

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DATA SPECIFIC INFORMATION FOR 'SOURCE_DATA_FIG_6.xlsx'
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Date of Creation: 2025-05-20
Description of Data: Electrophysiological parameters from whole-cell recordings + current injections from midbrain dopamine neurons (DAT-FlpO)
Number of Variables: 4
Variable List:
	A. Number of action potentials in response to current injected (f-I)
	Description: Each recorded neuron's number of action potentials evoked by a set amount of current injected through the electrode.
	# action potentials (integer values)
	
	B. Voltage-current relationship (I-V)
	Description: The membrane voltage in response to injected current values.
	mV

	C. Input resistance from I-V fit (Ri_fit)
	Description: The neuron membrane resistance, determined by linear fit of the sub threshold I-V response curve.
	mega-Ohms

	D. Input resistance measured (Ri_meas)
	Description: The neuron membrane resistance, measured/calculated from the test-pulse.
	mega-Ohms

	E. Resting-membrane potential (RMP)
	Description: The membrane potential without any current injected
	mV
	
	F. Rheobase current (I_rheobase)
	Description: The amount of current needed to evoke a single action potential
	pA
Number of Rows: 15
Column Names: 
	A. MOUSE_ID: the identifier of the mouse recorded
	integer
	B. GENOTYPE: the genotype of the mouse recorded
	txt
	C. SNc/VTA: whether the cell recorded was from the SNc or VTA
	SNc or VTA
	D. I_inj:X: the response to X pA of current injected
	varies by sheet (i.e., for sheet 'f-I' it corresponds to #action_potentials)
	E. Ri_fit: input resistance (from fit)
	mega-Ohm
	F. Ri_meas: input resistance (from test_pulse)
	mega-Ohm
	G. RMP: resting membrane potential for this cell
	mV
	H. I_rheobase: the rheobase recorded for this cell
	pA
Missing data codes:
	A. NaN: could not extract a value from the recorded trace (i.e., the current injection was so large that the voltage response trace was too large in amplitude to isolate action potentials)

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METHODOLOGY
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Description of methods used for data collection: All recordings were made using a Multiclamp 700B amplifier, the output of which was digitized at 10 kHz (Digidata 1440A). Series resistance was always <35 MΩ and was compensated up to 90%. Neurons were targeted with DIC microscopy and epifluorescence when appropriate.

Description of methods used for data processing: All data was processed using custom MATLAB code (see Key Resources Table), with values directly taken from MATLAB output into these Source Data files.

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DATA ACCESS AND SHARING
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Publications based on this dataset: Albarran et al. (in preparation)
Recommended citation for this dataset: TBD
License information: Creative Commons Attribution 4.0 International