CAIN and ClvR threshold-dependent drives parameters

We set each drive to their experimental parameters and varied the baseline germination rate (b) and fitness cost (s_g or s_s), while keeping all other seedbank parameters at their defaults. For a given drive, b, and s_g or s_s, we varied the drive homozygote introduction frequency from 0 to 1 (for modification drives) or from 0 to 0.5 (for suppression drives) in steps of 0.01 and tracked the rate of drive spread across 10 replicates. For modification drives, “drive spread” is defined as the drive ending the simulation at a higher frequency than it was released at, and for suppression drives, “drive spread” is defined as the drive either eliminating the population or ending the simulation at a higher frequency than it was released at. To run SLiM replicates and calculate the drive spread rate, we used Cornell University’s Computational Biology Department’s high-performance super-computing cluster. The following sections describe constants that must be set in the SLiM model (seedbank.slim) to simulate each threshold-dependent drive. All other constants should not be changed from their current values in the script. For each type of drive and fitness cost, we also include an example simulation that can be run at the command line.

1. Fitness cost reducing gamete viability (s_g > 0)

CAIN

SLiM constants

• L: 10
• b: for each s_g value, we explored b = 0.05, b = 0.2, b = 0.5, and b = 1.0.
• m: 0.0.
• d: 1.0
• q: 0.1
• K: 10000
• TRACK_FREQUENCIES: F
• BASELINE_OVULE_AVG: 60
• BASELINE_EFFECTIVE_POLLEN_AVG: 180
• MALE_FERTILITY_SUPPRESSION_DRIVE: F
• FEMALE_FERTILITY_SUPPRESSION_DRIVE: F
• CAIN: T
• s_g: we explored 3 fitness cost values: s_g = 0.05, s_g = 0.1, and s_g = 0.25.
• s_s: 0.0
• GERMLINE_CLEAVAGE_RATE_FEMALE: 0.941
• GERMLINE_CLEAVAGE_RATE_MALE: 0.984
• CAIN_PENETRANCE_RATE: 0.96
• INTRODUCTION_FREQUENCY: for a given b and s_g combination, we varied the the introduction frequency between 0.0 and 1.0 in steps of 0.01.
• HETEROZYGOUS_DROP: F
• PRESEED_R1_ALLELES: F

Example command line simulation

slim -d L=10 -d b=0.2 -d m=0.0 -d d=1.0 -d q=0.1 -d K=10000 -d TRACK_FREQUENCIES=F -d BASELINE_OVULE_AVG=60 -d BASELINE_EFFECTIVE_POLLEN_AVG=180 -d MALE_FERTILITY_SUPPRESSION_DRIVE=F -d FEMALE_FERTILITY_SUPPRESSION_DRIVE=F -d CAIN=T -d s_g=0.1 -d s_s=0.0 -d GERMLINE_CLEAVAGE_RATE_FEMALE=0.941 -d GERMLINE_CLEAVAGE_RATE_MALE=0.984 -d CAIN_PENETRANCE_RATE=0.96 -d INTRODUCTION_FREQUENCY=0.25 -d HETEROZYGOUS_DROP=F -d PRESEED_R1_ALLELES=F seedbank.slim

This simulates CAIN with s_g=0.1, b=0.2, an introduction frequency of 25% in drive homozygotes, and all other parameters set to their defaults. On a MacBook Pro (Apple M2 Pro CPU, 8GB RAM), this takes around 7 minutes to run. Note: if the SLiM file is not in your current directory, replace seedbank.slim with the path to the file.

ClvR

SLiM constants

• L: 10
• b: for each s_g value, we explored b = 0.05, b = 0.2, b = 0.5, and b = 1.0.
• m: 0.0.
• d: 1.0
• q: 0.1
• K: 10000
• TRACK_FREQUENCIES: F
• BASELINE_OVULE_AVG: 60
• BASELINE_EFFECTIVE_POLLEN_AVG: 180
• MALE_FERTILITY_SUPPRESSION_DRIVE: F
• FEMALE_FERTILITY_SUPPRESSION_DRIVE: F
• CAIN: F
• s_g: we explored 3 fitness cost values: s_g = 0.05, s_g = 0.1, and s_g = 0.25.
• s_s: 0.0
• GERMLINE_CLEAVAGE_RATE_FEMALE: 0.974
• GERMLINE_CLEAVAGE_RATE_MALE: 0.974
• CLVR_PENETRANCE_RATE: 1.0
• MATERNAL_CARRYOVER_RESCUE_RATE: 0.207
• INTRODUCTION_FREQUENCY: for a given b and s_g combination, we varied the the introduction frequency between 0.0 and 1.0 in steps of 0.01.
• HETEROZYGOUS_DROP: F
• PRESEED_R1_ALLELES: F

Example command line simulation

slim -d L=10 -d b=0.2 -d m=0.0 -d d=1.0 -d q=0.1 -d K=10000 -d TRACK_FREQUENCIES=F -d BASELINE_OVULE_AVG=60 -d BASELINE_EFFECTIVE_POLLEN_AVG=180 -d MALE_FERTILITY_SUPPRESSION_DRIVE=F -d FEMALE_FERTILITY_SUPPRESSION_DRIVE=F -d CAIN=F -d s_g=0.1 -d s_s=0.0 -d GERMLINE_CLEAVAGE_RATE_FEMALE=0.974 -d GERMLINE_CLEAVAGE_RATE_MALE=0.974 -d CLVR_PENETRANCE_RATE=1.0 -d MATERNAL_CARRYOVER_RESCUE_RATE=0.207 -d INTRODUCTION_FREQUENCY=0.25 -d HETEROZYGOUS_DROP=F -d PRESEED_R1_ALLELES=F seedbank.slim

This simulates ClvR with s_g=0.1, b=0.2, an introduction frequency of 25% in drive homozygotes, and all other parameters set to their defaults. On a MacBook Pro (Apple M2 Pro CPU, 8GB RAM), this takes a little over 7 minutes to run. Note: if the SLiM file is not in your current directory, replace seedbank.slim with the path to the file.

ClvR male suppression

SLiM constants

• L: 10
• b: for each s_g value, we explored b = 0.05, b = 0.2, b = 0.5, and b = 1.0.
• m: 0.0.
• d: 1.0
• q: 0.1
• K: 10000
• TRACK_FREQUENCIES: F
• BASELINE_OVULE_AVG: 60
• BASELINE_EFFECTIVE_POLLEN_AVG: 180
• MALE_FERTILITY_SUPPRESSION_DRIVE: T
• FEMALE_FERTILITY_SUPPRESSION_DRIVE: F
• CAIN: F
• s_g: we explored 3 fitness cost values: s_g = 0.05, s_g = 0.1, and s_g = 0.25.
• s_s: 0.0
• GERMLINE_CLEAVAGE_RATE_FEMALE: 0.974
• GERMLINE_CLEAVAGE_RATE_MALE: 0.974
• CLVR_PENETRANCE_RATE: 1.0
• MATERNAL_CARRYOVER_RESCUE_RATE: 0.207
• INTRODUCTION_FREQUENCY: for a given b and s_g combination, we varied the the introduction frequency between 0.0 and 0.5 in steps of 0.01.
• HETEROZYGOUS_DROP: F
• PRESEED_R1_ALLELES: F

Example command line simulation

slim -d L=10 -d b=1.0 -d m=0.0 -d d=1.0 -d q=0.1 -d K=10000 -d TRACK_FREQUENCIES=F -d BASELINE_OVULE_AVG=60 -d BASELINE_EFFECTIVE_POLLEN_AVG=180 -d MALE_FERTILITY_SUPPRESSION_DRIVE=T -d FEMALE_FERTILITY_SUPPRESSION_DRIVE=F -d CAIN=F -d s_g=0.1 -d s_s=0.0 -d GERMLINE_CLEAVAGE_RATE_FEMALE=0.974 -d GERMLINE_CLEAVAGE_RATE_MALE=0.974 -d CLVR_PENETRANCE_RATE=1.0 -d MATERNAL_CARRYOVER_RESCUE_RATE=0.207 -d INTRODUCTION_FREQUENCY=0.25 -d HETEROZYGOUS_DROP=F -d PRESEED_R1_ALLELES=F seedbank.slim

This simulates the ClvR male suppression drive with s_g=0.1, b=1.0, an introduction frequency of 25% in female drive homozygotes, and all other parameters set to their defaults. On a MacBook Pro (Apple M2 Pro CPU, 8GB RAM), this takes about 40 seconds to run. Note: if the SLiM file is not in your current directory, replace seedbank.slim with the path to the file.

ClvR female suppression

SLiM constants

• L: 10
• b: for each s_g value, we explored b = 0.05, b = 0.2, b = 0.5, and b = 1.0.
• m: 0.0.
• d: 1.0
• q: 0.1
• K: 10000
• TRACK_FREQUENCIES: F
• BASELINE_OVULE_AVG: 60
• BASELINE_EFFECTIVE_POLLEN_AVG: 180
• MALE_FERTILITY_SUPPRESSION_DRIVE: F
• FEMALE_FERTILITY_SUPPRESSION_DRIVE: T
• CAIN: F
• s_g: we explored 3 fitness cost values: s_g = 0.05, s_g = 0.1, and s_g = 0.25.
• s_s: 0.0
• GERMLINE_CLEAVAGE_RATE_FEMALE: 0.974
• GERMLINE_CLEAVAGE_RATE_MALE: 0.974
• CLVR_PENETRANCE_RATE: 1.0
• MATERNAL_CARRYOVER_RESCUE_RATE: 0.207
• INTRODUCTION_FREQUENCY: for a given b and s_g combination, we varied the the introduction frequency between 0.0 and 0.5 in steps of 0.01.
• HETEROZYGOUS_DROP: F
• PRESEED_R1_ALLELES: F

Example command line simulation

slim -d L=10 -d b=1.0 -d m=0.0 -d d=1.0 -d q=0.1 -d K=10000 -d TRACK_FREQUENCIES=F -d BASELINE_OVULE_AVG=60 -d BASELINE_EFFECTIVE_POLLEN_AVG=180 -d MALE_FERTILITY_SUPPRESSION_DRIVE=F -d FEMALE_FERTILITY_SUPPRESSION_DRIVE=T -d CAIN=F -d s_g=0.1 -d s_s=0.0 -d GERMLINE_CLEAVAGE_RATE_FEMALE=0.974 -d GERMLINE_CLEAVAGE_RATE_MALE=0.974 -d CLVR_PENETRANCE_RATE=1.0 -d MATERNAL_CARRYOVER_RESCUE_RATE=0.207 -d INTRODUCTION_FREQUENCY=0.25 -d HETEROZYGOUS_DROP=F -d PRESEED_R1_ALLELES=F seedbank.slim

This simulates the ClvR female suppression drive with s_g=0.1, b=1.0, an introduction frequency of 25% in male drive homozygotes, and all other parameters set to their defaults. On a MacBook Pro (Apple M2 Pro CPU, 8GB RAM), this takes about a minute and a half to run. Note: if the SLiM file is not in your current directory, replace seedbank.slim with the path to the file.

CAIN male suppression

SLiM constants

• L: 10
• b: for each s_g value, we explored b = 0.05, b = 0.2, b = 0.5, and b = 1.0.
• m: 0.0.
• d: 1.0
• q: 0.1
• K: 10000
• TRACK_FREQUENCIES: F
• BASELINE_OVULE_AVG: 60
• BASELINE_EFFECTIVE_POLLEN_AVG: 180
• MALE_FERTILITY_SUPPRESSION_DRIVE: T
• FEMALE_FERTILITY_SUPPRESSION_DRIVE: F
• CAIN: T
• s_g: we explored 3 fitness cost values: s_g = 0.05, s_g = 0.1, and s_g = 0.25.
• s_s: 0.0
• GERMLINE_CLEAVAGE_RATE_FEMALE: 0.941
• GERMLINE_CLEAVAGE_RATE_MALE: 0.984
• CAIN_PENETRANCE_RATE: 0.96
• INTRODUCTION_FREQUENCY: for a given b and s_g combination, we varied the the introduction frequency between 0.0 and 0.5 in steps of 0.01.
• HETEROZYGOUS_DROP: F
• PRESEED_R1_ALLELES: F

Example command line simulation

slim -d L=10 -d b=1.0 -d m=0.0 -d d=1.0 -d q=0.1 -d K=10000 -d TRACK_FREQUENCIES=F -d BASELINE_OVULE_AVG=60 -d BASELINE_EFFECTIVE_POLLEN_AVG=180 -d MALE_FERTILITY_SUPPRESSION_DRIVE=T -d FEMALE_FERTILITY_SUPPRESSION_DRIVE=F -d CAIN=T -d s_g=0.1 -d s_s=0.0 -d GERMLINE_CLEAVAGE_RATE_FEMALE=0.941 -d GERMLINE_CLEAVAGE_RATE_MALE=0.984 -d CAIN_PENETRANCE_RATE=0.96 -d INTRODUCTION_FREQUENCY=0.25 -d HETEROZYGOUS_DROP=F -d PRESEED_R1_ALLELES=F seedbank.slim

This simulates the CAIN male suppression drive with s_g=0.1, b=1.0, an introduction frequency of 25% in female drive homozygotes, and all other parameters set to their defaults. On a MacBook Pro (Apple M2 Pro CPU, 8GB RAM), this takes around 3 minutes to run. Note: if the SLiM file is not in your current directory, replace seedbank.slim with the path to the file.

2. Fitness cost reducing seed survival (s_s > 0)

CAIN

SLiM constants

• L: 10
• b: for each s_s value, we explored b = 0.05, b = 0.2, b = 0.5, and b = 1.0.
• m: 0.0.
• d: 1.0
• q: 0.1
• K: 10000
• TRACK_FREQUENCIES: F
• BASELINE_OVULE_AVG: 60
• BASELINE_EFFECTIVE_POLLEN_AVG: 180
• MALE_FERTILITY_SUPPRESSION_DRIVE: F
• FEMALE_FERTILITY_SUPPRESSION_DRIVE: F
• CAIN: T
• s_g: 0.0
• s_s: we explored 3 fitness cost values: s_s = 0.05, s_s = 0.1, and s_s = 0.25.
• GERMLINE_CLEAVAGE_RATE_FEMALE: 0.941
• GERMLINE_CLEAVAGE_RATE_MALE: 0.984
• CAIN_PENETRANCE_RATE: 0.96
• INTRODUCTION_FREQUENCY: for a given b and s_s combination, we varied the the introduction frequency between 0.0 and 1.0 in steps of 0.01.
• HETEROZYGOUS_DROP: F
• PRESEED_R1_ALLELES: F

Example command line simulation

slim -d L=10 -d b=1.0 -d m=0.0 -d d=1.0 -d q=0.1 -d K=10000 -d TRACK_FREQUENCIES=F -d BASELINE_OVULE_AVG=60 -d BASELINE_EFFECTIVE_POLLEN_AVG=180 -d MALE_FERTILITY_SUPPRESSION_DRIVE=F -d FEMALE_FERTILITY_SUPPRESSION_DRIVE=F -d CAIN=T -d s_g=0.0 -d s_s=0.25 -d GERMLINE_CLEAVAGE_RATE_FEMALE=0.941 -d GERMLINE_CLEAVAGE_RATE_MALE=0.984 -d CAIN_PENETRANCE_RATE=0.96 -d INTRODUCTION_FREQUENCY=0.25 -d HETEROZYGOUS_DROP=F -d PRESEED_R1_ALLELES=F seedbank.slim

This simulates CAIN with s_s=0.25, b=1.0, an introduction frequency of 25% in drive homozygotes, and all other parameters set to their defaults. On a MacBook Pro (Apple M2 Pro CPU, 8GB RAM), this takes about 1 minute to run. Note: if the SLiM file is not in your current directory, replace seedbank.slim with the path to the file.

ClvR

SLiM constants

• L: 10
• b: for each s_s value, we explored b = 0.05, b = 0.2, b = 0.5, and b = 1.0.
• m: 0.0.
• d: 1.0
• q: 0.1
• K: 10000
• TRACK_FREQUENCIES: F
• BASELINE_OVULE_AVG: 60
• BASELINE_EFFECTIVE_POLLEN_AVG: 180
• MALE_FERTILITY_SUPPRESSION_DRIVE: F
• FEMALE_FERTILITY_SUPPRESSION_DRIVE: F
• CAIN: F
• s_g: 0.0
• s_s: we explored 3 fitness cost values: s_s = 0.05, s_s = 0.1, and s_s = 0.25.
• GERMLINE_CLEAVAGE_RATE_FEMALE: 0.974
• GERMLINE_CLEAVAGE_RATE_MALE: 0.974
• CLVR_PENETRANCE_RATE: 1.0
• MATERNAL_CARRYOVER_RESCUE_RATE: 0.207
• INTRODUCTION_FREQUENCY: for a given b and s_s combination, we varied the the introduction frequency between 0.0 and 1.0 in steps of 0.01.
• HETEROZYGOUS_DROP: F
• PRESEED_R1_ALLELES: F

Example command line simulation

slim -d L=10 -d b=1.0 -d m=0.0 -d d=1.0 -d q=0.1 -d K=10000 -d TRACK_FREQUENCIES=F -d BASELINE_OVULE_AVG=60 -d BASELINE_EFFECTIVE_POLLEN_AVG=180 -d MALE_FERTILITY_SUPPRESSION_DRIVE=F -d FEMALE_FERTILITY_SUPPRESSION_DRIVE=F -d CAIN=F -d s_g=0.0 -d s_s=0.25 -d GERMLINE_CLEAVAGE_RATE_FEMALE=0.974 -d GERMLINE_CLEAVAGE_RATE_MALE=0.974 -d CLVR_PENETRANCE_RATE=1.0 -d MATERNAL_CARRYOVER_RESCUE_RATE=0.207 -d INTRODUCTION_FREQUENCY=0.25 -d HETEROZYGOUS_DROP=F -d PRESEED_R1_ALLELES=F seedbank.slim

This simulates ClvR with s_s=0.25, b=1.0, an introduction frequency of 25% in drive homozygotes, and all other parameters set to their defaults. On a MacBook Pro (Apple M2 Pro CPU, 8GB RAM), this takes about 45 seconds to run. Note: if the SLiM file is not in your current directory, replace seedbank.slim with the path to the file.

ClvR male suppression

SLiM constants

• L: 10
• b: for each s_s value, we explored b = 0.05, b = 0.2, b = 0.5, and b = 1.0.
• m: 0.0.
• d: 1.0
• q: 0.1
• K: 10000
• TRACK_FREQUENCIES: F
• BASELINE_OVULE_AVG: 60
• BASELINE_EFFECTIVE_POLLEN_AVG: 180
• MALE_FERTILITY_SUPPRESSION_DRIVE: T
• FEMALE_FERTILITY_SUPPRESSION_DRIVE: F
• CAIN: F
• s_g: 0.0
• s_s: we explored 3 fitness cost values: s_s = 0.05, s_s = 0.1, and s_s = 0.25.
• GERMLINE_CLEAVAGE_RATE_FEMALE: 0.974
• GERMLINE_CLEAVAGE_RATE_MALE: 0.974
• CLVR_PENETRANCE_RATE: 1.0
• MATERNAL_CARRYOVER_RESCUE_RATE: 0.207
• INTRODUCTION_FREQUENCY: for a given b and s_s combination, we varied the the introduction frequency between 0.0 and 0.5 in steps of 0.01.
• HETEROZYGOUS_DROP: F
• PRESEED_R1_ALLELES: F

Example command line simulation

slim -d L=10 -d b=1.0 -d m=0.0 -d d=1.0 -d q=0.1 -d K=10000 -d TRACK_FREQUENCIES=F -d BASELINE_OVULE_AVG=60 -d BASELINE_EFFECTIVE_POLLEN_AVG=180 -d MALE_FERTILITY_SUPPRESSION_DRIVE=T -d FEMALE_FERTILITY_SUPPRESSION_DRIVE=F -d CAIN=F -d s_g=0.0 -d s_s=0.25 -d GERMLINE_CLEAVAGE_RATE_FEMALE=0.974 -d GERMLINE_CLEAVAGE_RATE_MALE=0.974 -d CLVR_PENETRANCE_RATE=1.0 -d MATERNAL_CARRYOVER_RESCUE_RATE=0.207 -d INTRODUCTION_FREQUENCY=0.25 -d HETEROZYGOUS_DROP=F -d PRESEED_R1_ALLELES=F seedbank.slim

This simulates the ClvR male suppression drive with s_s=0.25, b=1.0, an introduction frequency of 25% in female drive homozygotes, and all other parameters set to their defaults. On a MacBook Pro (Apple M2 Pro CPU, 8GB RAM), this takes about 45 seconds to run. Note: if the SLiM file is not in your current directory, replace seedbank.slim with the path to the file.

ClvR female suppression

SLiM constants

• L: 10
• b: for each s_s value, we explored b = 0.05, b = 0.2, b = 0.5, and b = 1.0.
• m: 0.0.
• d: 1.0
• q: 0.1
• K: 10000
• TRACK_FREQUENCIES: F
• BASELINE_OVULE_AVG: 60
• BASELINE_EFFECTIVE_POLLEN_AVG: 180
• MALE_FERTILITY_SUPPRESSION_DRIVE: F
• FEMALE_FERTILITY_SUPPRESSION_DRIVE: T
• CAIN: F
• s_g: 0.0
• s_s: we explored 3 fitness cost values: s_s = 0.05, s_s = 0.1, and s_s = 0.25.
• GERMLINE_CLEAVAGE_RATE_FEMALE: 0.974
• GERMLINE_CLEAVAGE_RATE_MALE: 0.974
• CLVR_PENETRANCE_RATE: 1.0
• MATERNAL_CARRYOVER_RESCUE_RATE: 0.207
• INTRODUCTION_FREQUENCY: for a given b and s_s combination, we varied the the introduction frequency between 0.0 and 0.5 in steps of 0.01.
• HETEROZYGOUS_DROP: F
• PRESEED_R1_ALLELES: F

Example command line simulation

slim -d L=10 -d b=1.0 -d m=0.0 -d d=1.0 -d q=0.1 -d K=10000 -d TRACK_FREQUENCIES=F -d BASELINE_OVULE_AVG=60 -d BASELINE_EFFECTIVE_POLLEN_AVG=180 -d MALE_FERTILITY_SUPPRESSION_DRIVE=F -d FEMALE_FERTILITY_SUPPRESSION_DRIVE=T -d CAIN=F -d s_g=0.0 -d s_s=0.25 -d GERMLINE_CLEAVAGE_RATE_FEMALE=0.974 -d GERMLINE_CLEAVAGE_RATE_MALE=0.974 -d CLVR_PENETRANCE_RATE=1.0 -d MATERNAL_CARRYOVER_RESCUE_RATE=0.207 -d INTRODUCTION_FREQUENCY=0.25 -d HETEROZYGOUS_DROP=F -d PRESEED_R1_ALLELES=F seedbank.slim

This simulates the ClvR female suppression drive with s_s=0.25, b=1.0, an introduction frequency of 25% in male drive homozygotes, and all other parameters set to their defaults. On a MacBook Pro (Apple M2 Pro CPU, 8GB RAM), this takes about 2 minutes to run. Note: if the SLiM file is not in your current directory, replace seedbank.slim with the path to the file.

CAIN male suppression

SLiM constants

• L: 10
• b: for each s_s value, we explored b = 0.05, b = 0.2, b = 0.5, and b = 1.0.
• m: 0.0.
• d: 1.0
• q: 0.1
• K: 10000
• TRACK_FREQUENCIES: F
• BASELINE_OVULE_AVG: 60
• BASELINE_EFFECTIVE_POLLEN_AVG: 180
• MALE_FERTILITY_SUPPRESSION_DRIVE: T
• FEMALE_FERTILITY_SUPPRESSION_DRIVE: F
• CAIN: T
• s_g: we explored 3 fitness cost values: s_s = 0.05, s_s = 0.1, and s_s = 0.25.
• s_s: 0.0
• GERMLINE_CLEAVAGE_RATE_FEMALE: 0.941
• GERMLINE_CLEAVAGE_RATE_MALE: 0.984
• CAIN_PENETRANCE_RATE: 0.96
• INTRODUCTION_FREQUENCY: for a given b and s_s combination, we varied the the introduction frequency between 0.0 and 0.5 in steps of 0.01.
• HETEROZYGOUS_DROP: F
• PRESEED_R1_ALLELES: F

Example command line simulation

slim -d L=10 -d b=1.0 -d m=0.0 -d d=1.0 -d q=0.1 -d K=10000 -d TRACK_FREQUENCIES=F -d BASELINE_OVULE_AVG=60 -d BASELINE_EFFECTIVE_POLLEN_AVG=180 -d MALE_FERTILITY_SUPPRESSION_DRIVE=T -d FEMALE_FERTILITY_SUPPRESSION_DRIVE=F -d CAIN=T -d s_g=0.0 -d s_s=0.25 -d GERMLINE_CLEAVAGE_RATE_FEMALE=0.941 -d GERMLINE_CLEAVAGE_RATE_MALE=0.984 -d CAIN_PENETRANCE_RATE=0.96 -d INTRODUCTION_FREQUENCY=0.25 -d HETEROZYGOUS_DROP=F -d PRESEED_R1_ALLELES=F seedbank.slim

This simulates the CAIN male suppression drive with s_s=0.25, b=1.0, an introduction frequency of 25% in female drive homozygotes, and all other parameters set to their defaults. On a MacBook Pro (Apple M2 Pro CPU, 8GB RAM), this takes about 1.5 minutes to run. Note: if the SLiM file is not in your current directory, replace seedbank.slim with the path to the file.