Table 1. PCR Primers: The different primer pairs used to amplify the markers used in this study and their respective cycles.

Region	Name	Length	Source	Sequence 5 ’-3’		Cycle
COI	jgLCO1490	~650 bp	(Geller et al. 2013)	TITCIACIAAYCAYAARGAYATTGG	34x	3 min 96 °C
	jgHCO2198		(Geller et al. 2013)	TAIACYTCIGGRTGICCRAARAAYCA		60 s 95 °C
						60 s 48 °C
						60 s 72 °C
						6 min 72 °C
	CirrCO1F	~650 bp	(Weidhase et al. 2016)	TTTTTCTACTAACCATAAAGACATTG	34x	60 s 96 °C
	CirrCO1R		(Weidhase et al. 2016)	CCGAGGAAGTGTTGAGGGA		60 s 94 °C
						60 s 53 °C
						60 s 72 °C
						5 min 72 °C
	polyLCO	~650 bp	(Carr et al. 2011)	GAYTATWTTCAACAAATCATAAAG	5x	60 s 96 °C
	polyHCO		(Carr et al. 2011)	TAMACTTCWGGGTGACCAAARAATCA		40 s 95 °C
						40 s 46 °C
						60 s 72 °C
					35x	40 s 94 °C
						40 s 51 °C
						60 s 72 °C
						7 min 72 °C
	ZplankF1_t1	~650 bp	(Prosser et al. 2013)	tTCTASWAATCATAARGATATTG	29x	60 s 95 °C
	ZplankR1_t1		(Prosser et al. 2013)	TTCAGGRTGRCCRAARAATCA		40 s 94 °C
						40 s 51 °C
						60 s 72 °C
						5 min 72 °C
28S	28SC1	D1-D2	(Le et al. 1993)	ACCCGCTGAATTTAAGCAT	29x	60 s 96 °C
	28SD2	~750 bp	(Le et al. 1993)	TCCGTGTTTCAAGACGG		30 s 95 °C
						60 s 62 °C
						60 s 72 °C
						7 min 72 °C