# packages
library(metaDigitise)
library(magrittr)
library(tibble)
library(dplyr)
library(lubridate)
library(dpeatdecomposition)
library(dm)
library(RMariaDB)
library(ggplot2)
Connect to database
# connect to database
con <-
RMariaDB::dbConnect(
drv = RMariaDB::MariaDB(),
dbname = "dpeatdecomposition",
default.file = "~/my.cnf"
)
# get database as dm object
dm_dpeatdecomposition <-
dpeatdecomposition::dp_get_dm(con, learn_keys = TRUE)
Get most current IDs
id_last <-
list(
id_dataset =
dm_dpeatdecomposition %>%
dm::pull_tbl(datasets) %>%
dplyr::pull(id_dataset) %>%
tail(1),
id_sample =
dm_dpeatdecomposition %>%
dm::pull_tbl(samples) %>%
dplyr::pull(id_sample) %>%
tail(1),
id_measurement =
dm_dpeatdecomposition %>%
dm::pull_tbl(data) %>%
dplyr::pull(id_measurement) %>%
tail(1)
) %>%
purrr::map(function(.x) {
if(length(.x) == 0) {
0L
} else {
.x
}
})
Create directories
dir_name <- "d49"
dir_source <- "../raw_data/data/d49"
dir_target <- paste0("../derived_data/", id_last$id_dataset + 1L)
if(!dir.exists(dir_target)) {
dir.create(dir_target)
}
datasets <-
tibble::tibble(
id_dataset = id_last$id_dataset + 1L
)
citations_to_datasets <-
dplyr::bind_rows(
db_template_tables$citations_to_datasets,
tibble::tibble(
id_dataset = datasets$id_dataset,
id_citation = c("Manninen.2016")
)
)
# mass remaining (2013 experiment)
samples2 <-
dplyr::bind_rows(
# Sphagnum capillifolium
readxl::read_xlsx(paste0(dir_source, "/raw/Moss bags_September 2013-2014.xlsx"), sheet = 1L, skip = 4L) %>%
dplyr::select(1:11) %>%
dplyr::slice(1:26) %>%
setNames(nm = c("origin_sample_treatment", "id_plot", "plot_label", paste0("id_replicate_", 1:8))) %>%
dplyr::mutate(
dplyr::across(dplyr::starts_with("id_replicate"), as.numeric)
) %>%
tidyr::fill(
origin_sample_treatment, .direction = "down"
) %>%
tidyr::pivot_longer(
cols = dplyr::starts_with("id_replicate_"),
names_to = "id_replicate",
values_to = "mass_absolute"
) %>%
dplyr::mutate(
taxon_rank_value = "Sphagnum capillifolium"
),
# Sphagnum papillosum
readxl::read_xlsx(paste0(dir_source, "/raw/Moss bags_September 2013-2014.xlsx"), sheet = 1L, skip = 4L) %>%
dplyr::select(1:13) %>%
dplyr::slice(33:40) %>%
setNames(nm = c("origin_sample_treatment", "id_plot", "plot_label", paste0("id_replicate_", 1:10))) %>%
dplyr::mutate(
dplyr::across(dplyr::starts_with("id_replicate"), as.numeric)
) %>%
tidyr::fill(
origin_sample_treatment, .direction = "down"
) %>%
tidyr::pivot_longer(
cols = dplyr::starts_with("id_replicate_"),
names_to = "id_replicate",
values_to = "mass_absolute"
) %>%
dplyr::mutate(
taxon_rank_value = "Sphagnum papillosum"
),
) %>%
dplyr::mutate(
origin_sample_treatment =
dplyr::case_when(
origin_sample_treatment == "NO364" ~ "NO3 64",
TRUE ~ origin_sample_treatment
),
mass_relative_mass =
ifelse(is.na(mass_absolute), NA_real_, 1.0),
id_replicate =
id_replicate %>%
stringr::str_remove("^id_replicate_") %>%
as.numeric(),
sample_treatment = "control", #---note: samples incubate in control treatment
sample_type = "litter",
taxon_rank_name = "species",
taxon_organ = "shoots",
mesh_size_absolute = 0.71,
sampling_date = as.Date("2013-09-09"),
incubation_duration =
0 %>%
lubridate::dyears() %>%
lubridate::time_length(unit = "days"),
sampling_year = lubridate::year(sampling_date),
sampling_month = lubridate::month(sampling_date),
sampling_day = lubridate::day(sampling_date),
sample_microhabitat = "hummock",
sample_depth_upper = 3,
sample_depth_lower = 8,
id_dataset = datasets$id_dataset[[1]],
is_incubated = TRUE,
incubation_environment = "peat",
site_name = "Whim bog",
sampling_longitude = -3.271656,
sampling_latitude = 55.765479,
experimental_design =
paste0(
as.numeric(as.factor(site_name)), "_",
as.numeric(as.factor(origin_sample_treatment)), "_",
as.numeric(as.factor(sample_treatment)), "_",
as.numeric(as.factor(id_plot)), "_",
as.numeric(as.factor(id_replicate))
),
comments_samples = "Coordinates are only the approximate location of the site, but not the exact sampling point."
)
## New names:
## New names:
## • `treatment` -> `treatment...1`
## • `plot` -> `plot...2`
## • `letter code` -> `letter code...3`
## • `1` -> `1...4`
## • `2` -> `2...5`
## • `3` -> `3...6`
## • `4` -> `4...7`
## • `5` -> `5...8`
## • `6` -> `6...9`
## • `7` -> `7...10`
## • `8` -> `8...11`
## • `` -> `...12`
## • `` -> `...13`
## • `` -> `...14`
## • `` -> `...15`
## • `treatment` -> `treatment...16`
## • `plot` -> `plot...17`
## • `letter code` -> `letter code...18`
## • `1` -> `1...19`
## • `2` -> `2...20`
## • `3` -> `3...21`
## • `4` -> `4...22`
## • `5` -> `5...23`
## • `6` -> `6...24`
## • `7` -> `7...25`
## • `8` -> `8...26`
## • `` -> `...27`
## • `` -> `...28`
## • `` -> `...29`
## • `treatment` -> `treatment...30`
## • `plot` -> `plot...31`
## • `letter code` -> `letter code...32`
## • `1` -> `1...33`
## • `2` -> `2...34`
## • `3` -> `3...35`
## • `4` -> `4...36`
## • `5` -> `5...37`
## • `6` -> `6...38`
## • `7` -> `7...39`
## • `8` -> `8...40`
## • `` -> `...41`
## • `` -> `...42`
samples3 <-
dplyr::bind_rows(
# Sphagnum capillifolium
readxl::read_xlsx(paste0(dir_source, "/raw/Moss bags_September 2013-2014.xlsx"), sheet = 1L, skip = 4L) %>%
dplyr::select(16:26) %>%
dplyr::slice(1:26) %>%
setNames(nm = c("origin_sample_treatment", "id_plot", "plot_label", paste0("id_replicate_", 1:8))) %>%
dplyr::mutate(
dplyr::across(dplyr::starts_with("id_replicate"), as.numeric)
) %>%
tidyr::fill(
origin_sample_treatment, .direction = "down"
) %>%
tidyr::pivot_longer(
cols = dplyr::starts_with("id_replicate_"),
names_to = "id_replicate",
values_to = "mass_absolute"
) %>%
dplyr::mutate(
taxon_rank_value = "Sphagnum capillifolium"
),
# Sphagnum papillosum
readxl::read_xlsx(paste0(dir_source, "/raw/Moss bags_September 2013-2014.xlsx"), sheet = 1L, skip = 4L) %>%
dplyr::select(16:28) %>%
dplyr::slice(33:40) %>%
setNames(nm = c("origin_sample_treatment", "id_plot", "plot_label", paste0("id_replicate_", 1:10))) %>%
dplyr::mutate(
dplyr::across(dplyr::starts_with("id_replicate"), as.numeric)
) %>%
tidyr::fill(
origin_sample_treatment, .direction = "down"
) %>%
tidyr::pivot_longer(
cols = dplyr::starts_with("id_replicate_"),
names_to = "id_replicate",
values_to = "mass_absolute"
) %>%
dplyr::mutate(
taxon_rank_value = "Sphagnum papillosum"
),
) %>%
dplyr::mutate(
origin_sample_treatment =
dplyr::case_when(
origin_sample_treatment == "NO364" ~ "NO3 64",
TRUE ~ origin_sample_treatment
),
mass_relative_mass = mass_absolute/samples2$mass_absolute,
id_replicate =
id_replicate %>%
stringr::str_remove("^id_replicate_") %>%
as.numeric(),
sample_treatment = "control", #---note: samples incubate in control treatment
sample_type = "litter",
taxon_rank_name = "species",
taxon_organ = "shoots",
mesh_size_absolute = 0.71,
sampling_date = as.Date("2014-09-22"),
incubation_duration =
1 %>%
lubridate::dyears() %>%
lubridate::time_length(unit = "days"),
sampling_year = lubridate::year(sampling_date),
sampling_month = lubridate::month(sampling_date),
sampling_day = lubridate::day(sampling_date),
sample_microhabitat = "hummock",
sample_depth_upper = 3,
sample_depth_lower = 8,
id_dataset = datasets$id_dataset[[1]],
is_incubated = TRUE,
incubation_environment = "peat",
site_name = "Whim bog",
sampling_longitude = -3.271656,
sampling_latitude = 55.765479,
experimental_design =
paste0(
as.numeric(as.factor(site_name)), "_",
as.numeric(as.factor(origin_sample_treatment)), "_",
as.numeric(as.factor(sample_treatment)), "_",
as.numeric(as.factor(id_plot)), "_",
as.numeric(as.factor(id_replicate))
),
comments_samples = "Coordinates are only the approximate location of the site, but not the exact sampling point."
)
## New names:
## New names:
## • `treatment` -> `treatment...1`
## • `plot` -> `plot...2`
## • `letter code` -> `letter code...3`
## • `1` -> `1...4`
## • `2` -> `2...5`
## • `3` -> `3...6`
## • `4` -> `4...7`
## • `5` -> `5...8`
## • `6` -> `6...9`
## • `7` -> `7...10`
## • `8` -> `8...11`
## • `` -> `...12`
## • `` -> `...13`
## • `` -> `...14`
## • `` -> `...15`
## • `treatment` -> `treatment...16`
## • `plot` -> `plot...17`
## • `letter code` -> `letter code...18`
## • `1` -> `1...19`
## • `2` -> `2...20`
## • `3` -> `3...21`
## • `4` -> `4...22`
## • `5` -> `5...23`
## • `6` -> `6...24`
## • `7` -> `7...25`
## • `8` -> `8...26`
## • `` -> `...27`
## • `` -> `...28`
## • `` -> `...29`
## • `treatment` -> `treatment...30`
## • `plot` -> `plot...31`
## • `letter code` -> `letter code...32`
## • `1` -> `1...33`
## • `2` -> `2...34`
## • `3` -> `3...35`
## • `4` -> `4...36`
## • `5` -> `5...37`
## • `6` -> `6...38`
## • `7` -> `7...39`
## • `8` -> `8...40`
## • `` -> `...41`
## • `` -> `...42`
samples3 %>%
dplyr::filter(! stringr::str_detect(origin_sample_treatment, "PK$")) %>%
dplyr::group_by(taxon_rank_value, origin_sample_treatment) %>%
dplyr::summarize(
y = mean(mass_relative_mass, na.rm = T),
y_sd = sd(mass_relative_mass, na.rm = T),
.groups = "drop"
) %>%
dplyr::mutate(
origin_sample_treatment = factor(origin_sample_treatment, levels = c("control", "NO3 16", "NO3 64", "NH4 16", "NH4 64"))
) %>%
ggplot(aes(y = y, x = origin_sample_treatment, fill = taxon_rank_value)) +
geom_bar(stat="identity", position = position_dodge(0.9)) +
geom_errorbar(aes(ymin = y - y_sd, ymax = y + y_sd), position = position_dodge(0.9)) +
coord_cartesian(ylim = c(0.5, 1))
# litter collection
samples1 <-
samples2 %>%
dplyr::filter(!duplicated(paste0(taxon_rank_value, "_", origin_sample_treatment))) %>% #---note: all replicate litterbags prepared were prepared from homogenized litter collected from all plots
dplyr::mutate(
sample_treatment = origin_sample_treatment,
origin_sample_treatment = NA_character_,
id_plot = NA_character_,
plot_label = NA_character_,
id_replicate = NA_integer_,
sampling_day = NA_real_,
sampling_month = 8,
id_sample = seq_len(nrow(.)) + id_last$id_sample,
id_sample_origin = id_sample,
id_sample_parent = id_sample,
id_sample_incubation_start = NA_integer_,
is_incubated = FALSE,
incubation_environment = NA_character_,
experimental_design =
paste0(
as.numeric(as.factor(site_name)), "_",
as.numeric(as.factor(origin_sample_treatment)), "_",
as.numeric(as.factor(sample_treatment)), "_",
as.numeric(as.factor(id_plot)), "_",
as.numeric(as.factor(id_replicate))
),
comments_samples = paste0(comments_samples, " samples are mixtures of individual samples collected from all replicate plots of each treatment.")
)
# add missing ids
samples2 <-
dplyr::bind_rows(
samples2 %>%
dplyr::mutate(
type = "samples2"
),
samples3 %>%
dplyr::mutate(
type = "samples3"
)
)
samples2 <-
samples2 %>%
dplyr::mutate(
id_sample = seq_len(nrow(.)) + max(samples1$id_sample),
id_sample_origin =
dplyr::left_join(
samples2 %>%
dplyr::select(sample_type, taxon_rank_value, origin_sample_treatment) %>%
dplyr::rename(
sample_treatment = "origin_sample_treatment"
),
samples1 %>% dplyr::select(sample_type, taxon_rank_value, sample_treatment, id_sample),
by = c("sample_type", "taxon_rank_value", "sample_treatment")
) %>%
dplyr::pull(id_sample),
id_sample_incubation_start =
purrr::map_int(seq_len(nrow(.)), function(i) {
index <- paste0(sample_type, "_", taxon_rank_value, "_", site_name, "_", taxon_organ, "_", sample_depth_upper) == paste0(sample_type, "_", taxon_rank_value, "_", site_name, "_", taxon_organ, "_", sample_depth_upper)[[i]] & experimental_design == experimental_design[[i]] & incubation_duration == 0.0
id_sample[index]
}),
id_sample_parent =
purrr::map_int(seq_len(nrow(.)), function(i) {
index <- paste0(sample_type, "_", taxon_rank_value, "_", site_name, "_", taxon_organ, "_", sample_depth_upper) == paste0(sample_type, "_", taxon_rank_value, "_", site_name, "_", taxon_organ, "_", sample_depth_upper)[[i]] & experimental_design == experimental_design[[i]] & incubation_duration < incubation_duration[[i]]
if(! any(index)) {
id_sample_origin[[i]]
} else {
target_incubation_duration <- max(incubation_duration[index])
index <- index & incubation_duration == target_incubation_duration
id_sample[index]
}
})
)
# litter chemistry: I have two types of data: I have verified that `S.papillosum_CN litterbags_2013_copy.xlsx` contains measurements for individual litterbags for S. capillifolium for the 2013 experiment from @Manninen.2016. For S. papillosum, measurements were made only on litter pooled from individual plots of the same treatments (but the text does not say whether equal masses were used). However, if I understand the text correctly, also the litter used for the litterbag experiment was pooled in such a way and C and N measurements were made on aliquots of this pooled litter. Hence, I bind all values to the initial mass values
d49_litter_chemistry_1 <-
readxl::read_excel(paste0(dir_source, "/raw/S.papillosum_CN litterbags_2013_copy.xlsx"), sheet = 2L) %>%
dplyr::slice(1:36) %>%
dplyr::select(c(2, 8, 10)) %>%
setNames(c("id_plot", "C_relative_mass", "N_relative_mass")) %>%
dplyr::mutate(
id_plot = as.character(id_plot),
dplyr::across(
dplyr::ends_with("_mass_relative"),
function(.x) .x/100
),
incubation_duration = 0,
taxon_rank_value = "Sphagnum capillifolium"
)
d49_litter_chemistry_2 <-
readxl::read_excel(paste0(dir_source, "/raw/Moss bags_September 2013-2014.xlsx"), sheet = 4L, skip = 18L) %>%
dplyr::select(c(1, 2, 4)) %>%
setNames(c("sample_treatment", "C_relative_mass", "N_relative_mass")) %>%
dplyr::mutate(
sample_treatment =
dplyr::case_when(
stringr::str_detect(sample_treatment, "^NO") ~ stringr::str_replace(sample_treatment, "^NO", "NO3 "),
stringr::str_detect(sample_treatment, "^NH") ~ stringr::str_replace(sample_treatment, "^NH", "NH3 "),
sample_treatment == "con" ~ "control"
),
sample_treatment =
dplyr::case_when(
stringr::str_detect(sample_treatment, "PK$") ~ stringr::str_replace(sample_treatment, "PK$", " PK"),
TRUE ~ sample_treatment
),
dplyr::across(
dplyr::ends_with("_mass_relative"),
function(.x) .x/100
),
incubation_duration = 0,
taxon_rank_value = "Sphagnum papillosum"
)
## New names:
## • `` -> `...1`
## • `mass remaining %` -> `mass remaining %...3`
## • `mass remaining %` -> `mass remaining %...5`
## • `mass remaining %` -> `mass remaining %...7`
samples2 <-
dplyr::bind_rows(
dplyr::left_join(
samples2 %>%
dplyr::filter(taxon_rank_value == "Sphagnum capillifolium"),
d49_litter_chemistry_1,
by = c("taxon_rank_value", "incubation_duration", "id_plot")
),
dplyr::left_join(
samples2 %>%
dplyr::filter(taxon_rank_value == "Sphagnum papillosum"),
d49_litter_chemistry_2,
by = c("taxon_rank_value", "incubation_duration", "sample_treatment")
)
) %>%
dplyr::arrange(id_sample) %>%
dplyr::mutate(
C_absolute = NA_real_,
N_absolute = NA_real_
)
## combine
samples <-
dplyr::bind_rows(
db_template_tables$samples,
samples1 %>%
dplyr::mutate(
type = "samples1"
) %>%
dplyr::select(-sampling_date),
samples2
)
samples_to_samples <-
samples %>%
dplyr::filter(! id_sample %in% id_sample_origin) %>%
dplyr::mutate(
transition_description =
dplyr::case_when(
type %in% c("samples2") ~ "translocate",
type %in% c("samples3") ~ "wait",
TRUE ~ NA_character_
)
) %>%
dplyr::select(id_sample_parent, id_sample, transition_description) %>%
dplyr::rename(
id_sample_child = "id_sample"
)
d2 <-
samples2 %>%
tidyr::pivot_longer(
cols = dplyr::all_of(c("mass_absolute", "mass_relative_mass", "mesh_size_absolute", "C_relative_mass", "N_relative_mass", "C_absolute", "N_absolute")),
names_to = "attribute_name",
values_to = "value"
) %>%
dplyr::mutate(
id_measurement = seq_len(nrow(.)) + id_last$id_measurement,
id_measurement_numerator =
purrr::map_int(seq_len(nrow(.)), function(i) {
if(attribute_name[[i]] == "mass_relative_mass") {
id_measurement[id_sample == id_sample[[i]] & attribute_name == "mass_absolute"]
} else if (stringr::str_detect(attribute_name[[i]], pattern = "_relative_mass$")) {
id_measurement[id_sample == id_sample[[i]] & attribute_name == stringr::str_replace(attribute_name[[i]], "_relative_mass2?", "_absolute")]
} else {
NA_integer_
}
}),
id_measurement_denominator =
purrr::map_int(seq_len(nrow(.)), function(i) {
if(attribute_name[[i]] == "mass_relative_mass") {
id_measurement[id_sample == id_sample_incubation_start[[i]] & attribute_name == "mass_absolute"]
} else if (stringr::str_detect(attribute_name[[i]], pattern = "_relative_mass$")) {
id_measurement[id_sample == id_sample[[i]] & attribute_name == "mass_absolute"]
} else {
NA_integer_
}
}),
value_type = "point",
sample_size = 1L,
error = NA_real_,
error_type = "sd"
)
# combine
d <-
dplyr::bind_rows(
db_template_tables$data,
d2
) %>%
dplyr::select(dplyr::all_of(colnames(db_template_tables$data)))
experimental_design_format <-
tibble::tibble(
id_dataset = datasets$id_dataset,
file = paste0(id_last$id_dataset + 1L, "/experimental_design_format.csv"),
experimental_design_description = "`site_name`: Name of the site. `sample_treatment`: Character value indicating the form (NO3 or NH3) and dose (kg ha$^{-1}$ yr$^{-1}$) of N fertilization and whether in addition P and K were added (see @Manninen.2016 for details) during sample growth (for non-incubated litter samples) or during incubation (the dataset contains only data from samples incubated in control plots). `origin_sample_treatment` has the same format as `sample_treatment`, but defines for incubated samples in which plots the litter was grown. `id_plot`: Character representing the ID of te experimental plot (for *S. papillosum*, there is no plot label because all data and samples are pooled values from several plots, see @Manninen.2016). `id_replicate`: Integer value denoting replicate litter bags."
)
# csv file to export
experimental_design_format2 <-
samples %>%
dplyr::filter(! is.na(experimental_design)) %>%
dplyr::filter(! duplicated(experimental_design)) %>%
dplyr::select(experimental_design, site_name, origin_sample_treatment, sample_treatment, id_plot, id_replicate)
# export
write.csv(experimental_design_format2, paste0(dir_target, "/experimental_design_format.csv"), row.names = FALSE)
# list all tables
dm_insert_in <-
list(
datasets =
datasets %>%
dplyr::select(dplyr::all_of(colnames(dm_dpeatdecomposition$datasets))),
samples =
samples %>%
dplyr::select(dplyr::all_of(colnames(dm_dpeatdecomposition$samples))),
data =
d %>%
dplyr::select(dplyr::all_of(colnames(dm_dpeatdecomposition$data))),
samples_to_samples =
samples_to_samples %>%
dplyr::select(dplyr::all_of(colnames(dm_dpeatdecomposition$samples_to_samples))),
citations_to_datasets =
citations_to_datasets %>%
dplyr::select(dplyr::all_of(colnames(dm_dpeatdecomposition$citations_to_datasets))),
experimental_design_format =
experimental_design_format %>%
dplyr::select(dplyr::all_of(colnames(dm_dpeatdecomposition$experimental_design_format)))
)
# check whether all column names as present in table attributes
all_column_names <-
purrr::map(dm_insert_in, colnames) %>%
unlist() %>%
unique()
if(! all(all_column_names %in% (dm_dpeatdecomposition %>% dm::pull_tbl(attributes) %>% dplyr::pull(attribute_name)))) {
cond <- purrr::map_lgl(all_column_names, function(.x) ! .x %in% (dm_dpeatdecomposition %>% dm::pull_tbl(attributes) %>% dplyr::pull(attribute_name)))
RMariaDB::dbDisconnect(con)
stop(paste0("New `attribute_name`s discovered: ", paste(all_column_names[cond], collapse = ", ")))
}
all_data_attributes <- unique(dm_insert_in$data$attribute_name)
if(! all(all_data_attributes %in% (dm_dpeatdecomposition %>% dm::pull_tbl(attributes) %>% dplyr::pull(attribute_name)))) {
cond <- purrr::map_lgl(all_data_attributes, function(.x) ! .x %in% (dm_dpeatdecomposition %>% dm::pull_tbl(attributes) %>% dplyr::pull(attribute_name)))
stop(paste0("New `attribute_name`s discovered: ", paste(all_data_attributes[cond], collapse = ", ")))
RMariaDB::dbDisconnect(con)
}
# filter empty tables
dm_insert_in_check <-
dm_insert_in[purrr::map_lgl(dm_insert_in, function(x) nrow(x) > 0)] %>%
dm::as_dm() %>%
dp_dm_add_keys(dm_dpeatdecomposition)
# copy into dm_pmird
for(i in seq_along(dm_insert_in)) {
RMariaDB::dbAppendTable(con, name = names(dm_insert_in)[[i]], value = dm_insert_in[[i]])
}
RMariaDB::dbDisconnect(con)