Fluorescence microscopy and β-sheet structure detection: a) Peptide of helix No4 in solution with the Amytracker 630 dye. b) A CT fiber with the Amytracker 680 dye.



METHODS

The fluorescent dye Amytracker 630 or 680 (Ebba Biotech AB) (diluted 1:1000) was added to the peptide helix 4 (1 mg/mL) in solution or CT (3 mg/mL) after fiber formation. For the helix No4 peptide, 20 µL solution was placed on a microscope slide and then imaged using an inverted fluorescence microscope (Nikon Eclipse Ti, Nikon, Netherlands) and the filter “Texas Red” (Nikon). The CT fiber was moved to a 24-well plate and imaged while still in solution using a Leica DMI6000 B microscope.

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