3. Quick Tour of Virtual Frap
Virtual FRAP estimates and evaluates diffusion rates from FRAP experimental data in four steps. The four steps are intuitively guided through a wizard-based work flow:

  • Loading FRAP experimental data.

  • Defining regions of interest (ROI).

  • Selecting diffusion model which can either be a single diffusing component model or two diffusing components model.

  • Estimation of a primary diffusion rate and an optional secondary diffusion rate.

3.1 Virtual FRAP Main Interface

Figure 3-1 illustrates the Virtual FRAP 1.0 main user interface. It contains the raw FRAP data, the data analysis workflow, the best results and the spatial simulation all in one frame.

  1. Data Analysis Work Flow - Wizard based work flow guides users through the whole process from loading raw data to getting estimation results.

  2. Data Analysis Results Panel - It shows the best model and the model parameter values that fits the FRAP dataset.

  3. Spatial Simulation Toolbox - It runs the 2D spatial simulation based on the FRAP image with the estimated parameters and shows the simulation results together with the original data.

  4. FRAP Image Viewer - It displays the FRAP image based on the time series.


 
Figure 3-1: The Virtual Frap User Interface