The virtual FRAP batch run mode is created for users, who have conducted same experiments multiple times and want an average result over all experiments.
3.7.1 Starting the Virtual FRAP Batch Run Mode
Clicking on menu Tools -> Batch Run in the main user interface to start the Virtual FRAP batch run mode. Figure 3-15 shows the batch run mode user interface. The batch run interface has four components. They are,
- The Virtual FRAP batch run navigator – The tree structure lists all the FRAP documents in a batch run and also a link to results display when results are available. A toolbar at the end of the navigator window provides functions such as “Add a document”, “Delete a document” and “Delete all”.
- FRAP Image Viewer – It displays the FRAP image based on the time series.
- Results Panel – It shows the average parameter values over all the FRAP documents in a batch run.
- Job Status Panel – It shows job status while batch run is executing.
Figure 3-15 The Virtual FRAP Batch Run Mode
3.7.2 Adding a Document to Virtual FRAP Batch Run
Click on the “Add a
New Document” button
,
a wizard is popped up. This wizard basically is a combination of the “Load
FRAP data” wizard and “Define ROI” wizard in the main interface. The only
difference is that the “Add a New Document” wizard in batch run allows
loading a “.vfrap” document. To understand how to load a FRAP experimental
data and how to Define ROI, please refer to sections 3.2 and 3.3.
After adding a new document into the batch run, the new document’s name will appear in the batch run navigator tree. Upon selection of a document tree node, the image data related with the document will appear in the image viewer.
3.7.3 Deleting Document(s) from Batch Run
To delete a document
from the batch run, two steps have to be taken. Firstly, select the document
from the batch run navigator tree. Then, click on the “Delete a Document”
button 
. The
selected document will be deleted from the batch run. If there are batch run
results available, the results will be updated automatically. The results
will be unavailable if there is no document left in the batch run.
If users want to
delete all the documents in the batch run, they just need to click on
“Delete All Documents” button
.
All the documents and available results will be removed from batch run.
3.7.4 Executing Batch Run
When all the documents for a batch run
are added, users can start executing a batch run by clicking the batch run
button in the
toolbar of the batch run interface. A selected model type wizard will be
popped up before proceeding to batch run. Users are required to select only
one possible FRAP model for the batch run. To understand choosing a FRAP
model, please refer to section 3.4.
The batch run basically does estimation of parameters of selected model for each document involved and summarized over all the documents. The running job statuses are updated in the job status viewer simultaneously. Once the results are available, the results node will be updated in the batch run navigator.
3.7.5 Understanding Batch Run Results
When the results are ready, a “Results Available” node will be added to the navigator tree. Clicking on the tree node, the results display panel will be shown on top of the image viewer. Figure 3-16 shows results display panel.
Figure 3-16 Virtual FRAP Batch Run Results Panel
The batch run results panel has three tables. The one on top of the panel is the table showing the statistics over all the documents in a batch run. The statistics are mean, standard deviation, median, minimum and maximum of different parameters in a model.
The table in the middle lists all the
estimated parameter values for each document. Clicking on the details button
will lead
users to an estimate parameters panel, which users may refer to section 3.5.
The table at the bottom shows errors for each document calculated under selected ROIs. All the tables are able to be partially or wholly copied to clipboard to paste into other applications.