Contents

Overview

System Requirements

Overview

All software dependencies and operating systems using in ‘Microbiome convergence enables siderophore-secreting-rhizobacteria to improve iron nutrition and yield of peanut intercropped with maize’

System Requirements

Hardware requirements

This analysis require a standard computer with enough RAM to support the in-memory operations.

Software requirements

OS Requirements

This analysis is supported for windows and Linux, tested on the following systems:

Windows: 10

Linux: Ubuntu 20.10

R Package

 phyloseq=1.34.0 #microbiome analysis
 stats=4.2.2 #stats package
 car=3.1-1 #companion to applied regression
 forecast=8.2.0 ##box-cox数据变换 Data transformation
 ggpubr=0.4.0 #"ggplot2" based publication ready plots
 agricolae=1.3-5 #statistical procedures for agricultural research
 PMCMRplus=1.9.6 #calculate pairwise mutiple comparisons 
 rcompanion=2.4.21 #fucntions to support extension education proguram evaluaion
 vegan=2.5-7 # community ecology package
 psych=2.2.5 # correlation analysis
 ggplot2=3.4.0 #作图 plot
 ggpubr=0.5.0 #添加显著性标记, Add the significance marker
 dplyr=2.3.0 #数据清洗,Data cleaning
 plyr=1.8.8 #数据清洗,Data cleaning
 reshaped2=1.4.4 #数据清洗,Data cleaning
 ggthemes=4.2.4 #ggplot所用主题,Themes for ggplot2
 readxl=1.4.1 #读入 excel, read excel files
 ggsci=2.9 #配色,Themed color paletttes for "ggplto2"
 showtext=0.9-5 #字体设置, font setting
 extrafont=0.18 #Tools for using fonts
 sysfonts=0.8.8 #加载系统字体,loading the system fonts into R
 Cairo=1.6-0 #抗锯齿,anti-aliasing
 ggtree=3.4.4 #visualization of tree and annotation data
 stringr=1.5.0 #字符串处理string operations
 amplicon=1.14.2 # statistic and visualization for microbiome data

Linux Package

 QIIME2=2021.8 #microbiome analysis
 TBLASTN+=2.10.0 #Phylogenetic tree construction 
 MAFFT=7.487 #Multiple sequence alignment
 IQ-TREE2=2.1.4-beta #phylogenetic inference