MRI Tracking of Macrophages Labeled with Glucan Particles Entrapping a Water Insoluble Paramagnetic Gd-Based Agent.
Creators
- 1. Department of Molecular Biotechnology and Health Sciences and Molecular & Preclinical Imaging Centers, University of Torino, Torino, Italy; Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal and CNC - Center of Neurosciences and Cell Biology, University of Coimbra, Portugal
- 2. Department of Molecular Biotechnology and Health Sciences and Molecular & Preclinical Imaging Centers, University of Torino, Torino, Italy and ININCA (UBA-CONICET) Argentina
- 3. Department of Molecular Biotechnology and Health Sciences and Molecular & Preclinical Imaging Centers, University of Torino, Torino, Italy
- 4. FFUC - Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal and CNC - Center of Neurosciences and Cell Biology, University of Coimbra, Portugal
- 5. Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal; CNC - Center of Neurosciences and Cell Biology, University of Coimbra, Portugal and Coimbra Chemistry Center, University of Coimbra, Coimbra, Portugal
Description
PURPOSE: This study is aimed at demonstrating the in vivo potential of Gd(III)-loaded glucan particles (Gd-GPs) as magnetic resonance imaging (MRI)-positive agents for labeling and tracking phagocytic cells.
PROCEDURE: GPs were obtained from Saccharomyces cerevisae and loaded with the water-insoluble complex Gd-DOTAMA(C18)2. The uptake kinetics of Gd-GPs by murine macrophages was studied in vitro and the internalization mechanism was assessed by competition assays. The in vivo performance of Gd-GPs was tested at 7.05 T on a mouse model of acute liver inflammation.
RESULTS: The minimum number of Gd-GPs-labeled J774.A1 macrophages detected in vitro by MRI was ca. 300 cells/μl of agar, which is the lowest number ever reported for cells labeled with a positive T1 agent. Intravenous injection of macrophages labeled with Gd-GPs in a mouse model of liver inflammation enabled the MRI visualization of the cellular infiltration in the diseased area.
CONCLUSIONS: Gd-GPs represent a promising platform for tracking macrophages by MRI as a T1 alternative to the golden standard T2-based iron oxide particles.
Files
Figueiredo_MolImagingBiol_2013-P05-AAM.pdf
Files
(6.3 MB)
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